Summary
Horseradish peroxidase, perfused into the lateral ventricle of chick brain, freely and slowly diffuses through the cerebral extracellular spaces. The layer of astrocytic end-feet surrounding blood capillaries does not constitute a barrier to the tracer which permeates the basal lamina, diffuses between the pericytic cells and finally accumulates in the intercellular space beneath the tight junctions between contiguous endothelial cells. No evidence was found for transport by micropinocytotic vesicles from the cerebral parenchyma to the capillary lumen.
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Becker, N. H., Hirano, A., Zimmerman, H. M.: Observations of the distribution of exogenous peroxidase in the rat cerebrum. J. Neuropath. exp. Neurol. 27, 439–452 (1968)
Bodenheimer, T. S., Brightman, M. W.: A blood-brain barrier to peroxidase in capillaries surrounded by perivascular spaces. Amer. J. Anat. 122, 249–267 (1968)
Brightman, M. W.: The intracerebral movement of proteins injected into blood and cerebrospinal fluid of mice. Progr. Brain Res. 29, 19–37 (1967)
Brightman, M. W., Klatzo, I., Olsson, Y., Reese, T. S.: The blood-brain barrier to proteins under normal and pathological conditions. J. neurol. Sci. 10, 215–239 (1970)
Brightman, M. W., Reese, T. S.: Junctions between intimately apposed cell membranes in the vertebrate brain J. Cell Biol. 40, 648–677 (1969)
Delorme, P.: Etude ultrastructurale du développement des capillaires, de leur environnement et des modifications de leur perméabilité à la peroxydase du Raifort dans le télencéphale du Poulet au cours de l'embryogénèse et de la croissance postnatale. Thèse de Doctorat ès-Sciences Naturelles, Novembre 1971, Nancy, France
Delorme, P., Gayet, J., Grignon, G.: Ultrastructural study on transcapillary exchanges in the developing telencephalon of the chicken. Brain Res. 22, 269–283 (1970)
Foncin, J.-F.: Comportement des macromolécules de peroxydase au voisinage des capillaires cérébraux humains. Acta neurol. 18, 242–245 (1973)
Graham, R. C., Karnovsky, M. J.: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem. 14, 291–301 (1966)
Kuffler, S. W., Nicholls, J. G.: The physiology of neuroglial cells. Ergebn. Physiol. 57, 1–90 (1966)
Reese, T. S., Karnovsky, M. J.: Fine structural localization of a blood-brain barrier to exogenous peroxidase. J. Cell Biol. 34, 207–217 (1967)
Stensaas, L. J., Stensaas, S. S.: Astrocytic neuroglial cells, oligodendrocytes and microgliocytes in the spinal cord of the toad. II. Electron microscopy. Z. Zellforsch. 86, 184–213 (1968)
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Thanks are due to Mrs. Thérèse Jacquot and Christiane Math and Miss Marie-Christine Thuillier for technical assistance. — This research was supported by grants from the Centre National de la Recherche Scientifique (E.R.A. n∘ 331) and from the Fondation pour la Recherche Médicale Française.
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Delorme, P., Gayet, J. & Grignon, G. Diffusion of horseradish peroxidase perfused through the lateral ventricle of the chick telencephalon. Cell Tissue Res. 157, 535–540 (1975). https://doi.org/10.1007/BF00222605
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DOI: https://doi.org/10.1007/BF00222605