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Differential binding characteristics and applications of dGalβ1→3dGalNAc specific lectins

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Summary

The binding properties of Arachis hypogaea (PNA), Bauhinia vurpurea alba (BPL), Maclura pomifera (MPL) and Sophora japonica (SJL) lectins were studied by quantitative precipitin and precipitin inhibition assays, demonstrating them to be most specific for dGalβ1→3dGalNAc residues. Additionally, each lectin had its own binding characteristic such as different binding abilities to dGalβ1→4dGlcNAc or dGalβ1→3dGlcNAcβ1→linked oligosaccharides, and/or dGalNAcα1→linked to the Ser or Thr of the protein moiety. These differential binding characteristics can be used for investigating fine differences of the carbohydrate structure of the glycoconjugates, especially those having dGalβ1→3dGalNAc residues as terminal non-reducing ends.

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Abbreviations

dGal:

d-galactopyranose

dMan:

d-mannopyranose

dGalNAc:

2-acetamido-2-deoxy-d-galacto-pyranose

dGlcNAc:

2-acetamino-2-deoxy-d-glucopyranose

LFuc:

L-fucose

NeuNAc:

N-acetylneuraminic acid

Ser:

serine

Thr:

Threonine

RCA:

Ricinus communis agglutinin

SBA:

Soy bean agglutinin (Glycine max)

HPA:

Helix pomatia agglutinin

DBL:

Dolichos biflorus lectin

GCL:

Geodia cydonium lectin

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  1. Department of Veterinary Pathology, College of Veterinary Medicine, Texas A&M University, 77843, College Station, TX, USA

    Albert M. Wu

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Wu, A.M. Differential binding characteristics and applications of dGalβ1→3dGalNAc specific lectins. Mol Cell Biochem 61, 131–1411 (1984). https://doi.org/10.1007/BF00222491

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