Summary
Partition behavior of liposomes of different composition in the aqueous Ficoll-Dextran biphasic system was examined. The relative hydrophobicity of the liposomes was estimated. The effect of cholesterol on the solvent interactions of lecithin and sphingomyelin liposomes was studied. It is found that the effect of phosphatidylethanolamine on the hydrophobic character of lecithin liposomes exceeds that produced by cholesterol.
The relative hydrophobicity of all the liposomes examined was found to be independent of the ionic composition of the system containing varied amounts of NaCl and Na-phosphate buffer, pH 7.4. The hydrophobic character of the liposomes under study is shown to increase in the following order: phosphatidylserine < phosphatidylethanolamine < phosphatidylcholine < sphingomyelin. Hydrophobic properties of the liposomes are compared to those of human red cells. It is suggested that the dependence of the hydrophobic character of membrane particles on the ionic composition of an aqueous environment can be of fundamental importance for the selective distribution of the particles in vivo.
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References
Fisher, D., 1981. Biochem. J. 196: 1.
Walter, H., 1977. Methods Cell Separation 1: 307.
Eriksson, E. and Johansson, G., 1979. In: Cell Populations (Reid, E., ed.), pp. 81–90. Ellis Horwood, Chichester.
Zaslavsky, B. Yu., Miheeva, L. M. and Rogozhin, S. V., 1979. Biochim. Biophys. Acta 588: 89.
Martin, Y. C., 1981. J. Med. Chem. 24: 229.
Tagesson, C., Stendahl, O. and Magnusson, K. E., 1977. Studia Biophys. 64: 151.
Eriksson, E. and Albertsson, P. Å., 1978. Biochim. Biophys. Acta 507: 425.
Dawson, R. M., 1963. Biochem. J. 88: 414.
Kinsky, S. C., Haxby, J. A., Zopf, D., Alving, C. A. and Kinsky, C. B., 1969. Biochemistry 8: 4149.
Batzri, S. and Korn, E. D., 1973. Biochim. Biophys. Acta 298: 1015.
Zaslavsky, B. Yu., Miheeva, L. M., Rogozhin, S. V., Borsova, L. V. and Kosinez, G. I., 1980. Biochim. Biophys. Acta 597: 53.
Kremer, J. M. H., Esker, M. W., Patmamancharan, C. and Wiersema, P. H., 1977. Biochemistry 16: 3932.
Papahadjopoulos, D. and Kimelberg, H. K., 1973. Progress Surf. Sci. 4: 141.
Yeagle, P. L., Hutton, W. C., Huang, C. and Martin, R. B., 1977. Biochemistry 16: 4344.
Jendrasiak, G. L. and Mendible, J. C., 1976. Biochim. Biophys. Acta 424: 133.
Newman, G. C. and Huang, C., 1975. Biochemistry 14:3363.
Zaslavsky, B. Yu., Borovskaya, A. A., Davidovich, Yu. A. and Rogozhin, S. V., 1981. Chem. Phys. Lipids 28: 181.
Hertz, R. and Barenholz, Y., 1977. J. Colloid Interface Sci. 60: 188.
Brockerhoff, H., 1974. Lipids 9: 645.
Demel, R. A., Jansen, J. W. C. M., Van Dijck, P. W. M. and Van Deenen, L. L. M., 1977. Biochim. Biophys. Acta 465: 1.
Walter, H., Kroh, E. J., Webber, T. J., Ascher, G. S. and Morin, R. J., 1979. Biochim. Biophys. Acta 550: 138.
Zaslavsky, B. Yu., Miheeva, L. M. and Rogozhin, S. V., 1981. J. Chromatogr. 216: 103.
Zaslavsky, B. Yu., Miheeva, L. M. and Rogozhin, S. V., 1981. J. Chromatogr. 212: 13.
Zaslavsky, B. Yu., Miheeva, L. M., Mestechkina, N. M. and Rogozhin, S. V., 1982. J. Chromatogr. 253: 139.
Zaslavsky, B. Yu., Mestechkina, N. M., Miheeva, L. M. and Rogozhin, S. V., 1982. J. Chromatogr. 240: 21.
Zaslavsky, B. Yu., Mestechkina, N. M. and Rogozhin, S. V., 1979. Biochim. Biophys. Acta 579: 463.
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Zaslavsky, B.Y., Borovskaya, A.A. & Rogozhin, S.V. Effect of lipid composition on hydrophobic properties of liposomes. Mol Cell Biochem 60, 131–136 (1984). https://doi.org/10.1007/BF00222483
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DOI: https://doi.org/10.1007/BF00222483