Abstract
Atlantic salmon (Salmo salar) cDNAs encoding the major histocompatibility complex (Mhc-Sasa) class II β chain were isolated from a leucocyte library by a polymerase chain reaction (PCR) approach. Three different cDNAs (c144, c22, and c157) encoding the entire mature β chain have been analyzed. Clone c144 differs from clone c157 in 12.6% of the nucleotides in the β1-encoding region. The corresponding differences between clones c144 and c22, and clones c22 and c157, are 10.3% and 5.2%, respectively. This variation is, at least in part, most likely attributable to allelism. The similarity indices between the highly conserved β2 domains from Atlantic salmon and corresponding sequences from humans (DQβ), chicken (BLβ), carp (TLAIIβ-1), and rainbow trout (O. M. No. 55) are 45%, 40%, 66%, and 97%, respectively. Variable residues in the β1 domains from Atlantic salmon correspond with polymorphic sites of β1 domains from higher vertebrates. The frequency of substitutions in the β1-encoding region exceeds that in the 3′-untranslated (UT) region with several folds, indicating extensive β1 polymorphism in Atlantic salmon.
The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers (C 144) X 70165, (C 157) X 70166, and (C 22) X 70167.
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This study was supported by grants from the Norwegian Fisheries Research Council (NFFR) and the Norwegian Agricultural Research Council (NLVF).
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Hordvik, I., Grimholt, U., Fosse, V.M. et al. Cloning and sequence analysis of cDNAs encoding the MHC class II β chain in Atlantic salmon (Salmo salar). Immunogenetics 37, 437–441 (1993). https://doi.org/10.1007/BF00222467
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DOI: https://doi.org/10.1007/BF00222467