Skip to main content
SpringerLink
Log in

Structure and organization of the B genome based on a linkage map in Brassica nigra

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

We constructed a genetic map on Brassica nigra based on a segregating population of 83 F2 individuals. Three different types of molecular markers were used to build the map including isozymes, restriction fragment length polymorphisms (RFLP), and random amplified polymorphic DNA (RAPD). The final map contained 124 markers distributed in 11 linkage groups. The map covered a total distance of 677 cM with the markers distributed within a mean distance of 5.5cM. Of the sequences found in the B. nigra map, 40% were duplicated and organized into three different types of arrangements. They were either scattered throughout the genome, organized in tandem, or organized in blocks of duplicated loci conserved in more than 1 linkage group.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anderson JA, Ogihara Y, Sorrells ME, Tanksley SD (1992) Development of a chromosomal arm map for wheat based on RFLP markers. Theor Appl Genet 83:1035–1043

    Google Scholar 

  • Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Rep 9:208–219

    Google Scholar 

  • Arús P, Chevre AM, Derlourme R, Feber F, Kerlan MC, Margale E, Quiros CF (1991) Isozyme nomenclature for eight enzyme systems in three Brassica species. In: Rapeseed in a changing world: breeding, biotechnology, vol 4. (GCIRC 8th Int Rapeseed Congr). Saskatoon, Saskatchewan, Canada, pp 1061–1066

  • Bernatzky R, Tanksley SD (1986) Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics 112:887–898

    Google Scholar 

  • Chang C, Bowman JL, DeJohn AW, Lander ES, Meyerowitz EM (1988) Restriction fragment length polymorphism linkage map for Arabidopsis thaliana. Proc Natl Acad Sci USA 85:6856–6860

    Google Scholar 

  • Chevre AM, This P, Eber F, Deschamps M, Renard M, Delseny M, Quiros CF (1991) Characterization of disomic addition lines Brassica napus-Brassica nigra by isozyme, fatty acid, and RFLP markers. Theor Appl Genet 81:43–49

    Google Scholar 

  • Chyi YS, Hoenecke ME, Sernyk JL (1992) A genetic linkage map of restriction fragment length polymorphism loci for Brassica rapa (syn. campestris). Genome 35:746–757

    Google Scholar 

  • Comai L, Baden CS, Harada JJ (1989) Coordinate expression of transcriptionally regulated isocitrate lyase and malate synthase genes in Brassica napus. Plant Cell 1:293–300

    Google Scholar 

  • Crouch ML, Tenbarge KM, Simon AE, Ferl R (1983) cDNA clones from Brassica napus seed storage proteins: evidence from nucleotide sequence analysis that both subunits of napin are cleaved from a precursor polypeptide. J Mol Appl Genet 2:273–283

    Google Scholar 

  • Durham RE, Liou PC, Gmitter FG, Moore GA (1992) Linkage of restriction fragment length polymorphisms and isozymes in Citrus. Theor Appl Genet 84:39–48

    Google Scholar 

  • Feinberg AP, Volgelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13

    CAS  PubMed  Google Scholar 

  • Fidgore SS, Kennard WC, Song KM, Slocum MK, Osborn TC (1988) Assessment of the degree of restriction fragment length polymorphism in Brassica. Theor Appl Genet 75:833–840

    Google Scholar 

  • Fisher RL, Golberg RB (1982) Structure and flanking regions of soybean seed protein genes. Cell 29:651–660

    Google Scholar 

  • Gebhardt C, Ritter E, Debener T, Schachtschabel U, Walkemeier B, Uhrig H, Salamini F (1989) RFLP analysis and linkage mapping in Solanum tuberosum. Theor Appl Genet 78:65–75

    Google Scholar 

  • Gerlach WL, Bedbrook JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7:1869–1885

    CAS  PubMed  Google Scholar 

  • Gottlieb LD (1983) Isozyme number and phylogeny. In: Jesen U, Fairbrothers DE (eds) Proteins and nucleic acids in plant systematics. Springer, Berlin Heidelberg New York, pp 209–221

    Google Scholar 

  • Graner A, Jahoor A, Schondelmaier J, Siedler H, Pillen K, Fischbeck G, Wenzel G, Herrmann RG (1991) Construction of an RFLP map on barley. Theor Appl Genet 83:250–256

    Google Scholar 

  • Harada JJ, Baden CS, Lomai L (1988) Spatially regulated genes expressed during seed germination and postgerminative development are activated during embryogeny. Mol Gen Genet 212:466–473

    Google Scholar 

  • Havey MJ, Muehlbauer FJ (1989) Linkages between restriction fragment length, isozyme, and morphological markers in lentil. Theor Appl Genet 77:395–401

    Google Scholar 

  • Helentjaris T (1985) Restriction fragment polymorphisms as probes for plant diversity and their development as a tool for applied plant breeding. Plant Mol Biol 5:109–118

    Google Scholar 

  • Helentjaris T, Slocum M, Wright S, Schaefer A, Nienhuis J (1986) Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphisms. Theor Appl Genet 72:761–769

    Google Scholar 

  • Helentjaris T, Weber D, Wright S (1988) Identification of the genomic locations of duplicated nucleotide sequences in maize by the analysis of restriction fragment length polymorphisms. Genetics 118:353–363

    CAS  Google Scholar 

  • Hemingway JS (1979) Mustards. In: Simmonds NW (eds) Evolution of crop plants. Longman, London, pp 56–59

    Google Scholar 

  • Hosaka K, Kianian SF, McGrath JM, Quiros CF (1990) Development and chromosomal localization of genome specific DNA markers of Brassica and the evolution of amphidiploid and n = 9 diploid species. Genome 33:131–142

    CAS  Google Scholar 

  • Hu J, Quiros CF (1991) Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep 10:505–511

    Google Scholar 

  • Jarrell DC, Roose ML, Traugh SN, Kupper RS (1992) A genetic map of citrus based on the segregation of isozymes and RFLPs in an intergeneric cross. Theor Appl Genet 84:49–56

    Google Scholar 

  • Kesseli RV, Paran I, Michelmore RW (1992) Efficient mapping of specifically targeted genomic regions and the tagging of these regions with reliable PCR-based genetic markers. In: Applications of RAPD technology to plant breeding. Joint plant breeding symposia series). Crop Science Society of America, Minneapolis, Minnesota, pp 31–36

    Google Scholar 

  • Kianian SF, Quiros CF (1992) Generation of a Brassica oleracea composite RFLP map: linkage arrangements among various populations and evolutionary implications. Theor Appl Genet 84:544–554

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln S, Newburg L (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Landry BS, Kesseli R, Leung H, Michelmore RW (1987a) Comparison of restriction endonucleases and sources of probes for their efficiency in detecting restriction fragment length polymorphisms in lettuce (Lactuca sativa L.). Theor Appl Genet 74:646–653

    Google Scholar 

  • Landry BS, Kesseli RV, Farrara B, Michelmore RW (1987b) A genetic map of lettuce (Lactuca sativa L.) with restriction fragment length polymorphism, isozyme, disease resistance and morphological markers. Genetics 116:331–337

    Google Scholar 

  • Landry BS, Hubert N, Etoh T, Harada JJ, Lincoln SE (1991) A genetic map for Brassica napus based on restriction fragment length polymorphism detected with expressed DNA sequences. Genome 34:543–552

    Google Scholar 

  • Landry BS, Hubert N, Crete R, Chang MS, Lincoln SE, Etoh T (1992) A genetic map for Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora brassicae (Woronin). Genome 35:409–420

    CAS  Google Scholar 

  • Lincoln S, Daly M, Lander E (1992) Constructing genetic maps with MAPMAKER/EXP 3.0 Whitehead Institute Technical Report

  • Maniatis TE, Fritsch F, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.

    Google Scholar 

  • McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829

    CAS  Google Scholar 

  • McGrath JM, Quiros CF (1991) Inheritance of isozyme and RFLP markers in Brassica campestris and comparison with B. oleracea. Theor Appl Genet 82:668–673

    Google Scholar 

  • Nasrallah JB, Kao TH, Goldberg ML, Nasrallah ME (1985) A cDNA clone encoding an S-locus specific glycoprotein from Brassica oleracea. Nature 318:263–267

    Google Scholar 

  • Ohno S (1970) Evolution by gene duplication. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Paterson AH, DeVerna JW, Lanini B, Tanksley SD (1990) The mapping of quantitative trait loci using selected overlapping recombinant chromosomes, in an interspecies cross of tomato. Genetics 124:735–742

    Google Scholar 

  • Pichersky E (1990) Nomad DNA-a model for movement and duplication of DNA sequences in plant genomes. Plant Mol Biol 15:437–448

    Google Scholar 

  • Pillen K, Steinrücken G, Wricke G, Herrmann RG, Jung C (1992) A linkage map of sugar beet (Beta vulgaris L.). Theor Appl Genet 84:129–135

    Google Scholar 

  • Prakash S, Hinata K (1980) Taxonomy, cytogenetics and origin of crop Brassicas, a review. Opera Bot 55:1–57

    Google Scholar 

  • Quiros CF, Ochoa O, Kianian SF, Douches DS (1987) Analysis of the Brassica oleracea genome by the generation of B. rapa-oleracea chromosome addition lines: characterization by isozymes and rDNA genes. Theor Appl Genet 74:758–766

    Google Scholar 

  • Quiros CF, McGrath JM, Kianian SF, This P, Ochoa O (1989) Cytogenetic stocks in Brassica: addition lines and genome evolution. In: Proc 5th Crucifer Genetics Workshop. University of California, Davis, pp 4–7

    Google Scholar 

  • Quiros CF, Hu J, This P, Chevre AM, Delseny M (1991) Development and chromosomal localization of genome-specific markers by polymerase chain reaction in Brassica. Theor Appl Genet 82:627–632

    CAS  Google Scholar 

  • Richaria RH (1937a) Cytological investigations of Raphanus sativus, Brassica oleracea and their F1 hybrids and F2 hybrids. J Genet 34:45–55

    Google Scholar 

  • Richaria RH (1937b) Cytological investigation of 10-chromosome species of Brassica and their F1 hybrids. J Genet 34:45–55

    Google Scholar 

  • Robbelen G (1960) Beitrage zur Analyse des Brassica-genoms. Chromosomal 11:205–228

    Google Scholar 

  • Simon AE, Tenbarge KM, Scofield SR, Finkelstein RR, Crouch ML (1985) Nucleotide sequence of a cDNA clone of Brassica napus 12s storage protein shows homology with legumin from Pisum sativum. Plant Mol Biol 5:191–201

    Google Scholar 

  • Sjödin C, Glimelius K (1989) Transfer of resistance against Phoma lingarn to Brassica napus by asymmetric somatic hybridization combined with toxin selection. Theor Appl Genet 78:513–520

    Google Scholar 

  • Slocum MK, Fidgore SS, Kennard WC, Suzuki JY, Osborn TC (1990) Linkage arrangement of restriction fragment length polymorphism loci in Brassica oleracea. Theor Appl Genet 80:57–64

    Google Scholar 

  • Song KM, Suzuki JY, Slocum MK, Williams PH, Osborn TC (1991) A linkage map of Brassica rapa (syn. campestris) based on restriction fragment length polymorphism loci. Theor Appl Genet 82:296–304

    Google Scholar 

  • Struss D, Bellin U, Robbelen G (1991a) Development of B-genome chromosome addition lines of B. napus using different interspecific Brassica hybrids. Plant Breed 106:209–214

    Google Scholar 

  • Struss D, Quiros CF, Robbelen G (1991b) Construction of different B-genome addition lines of Brassica napus L. Rapeseed in a changing world: breeding, biotechnology, vol 2. (GCIRC 8th Int Rapeseed Congr). Saskatoon, Saskatchewan, Canada, pp 358–362

  • Suiter KA, Wendel JF, Case JS (1983) Linkage-1: a pascal computer program for the detection and analysis of genetic linkage. J Hered 74:203–204

    Google Scholar 

  • UN (1935) Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jpn J Genet 7:784–794

    Google Scholar 

  • This P (1992) Characterisation et development de marqueurs moléculaires en vue de l'analyse de lignees d'addition Brassica napus-Brassica nigra et Diplotaxis erucoides-Brassica nigra. PhD thesis, University of Perpignan, France

    Google Scholar 

  • Truco MJ (1986) Estudi de la Variabilitat isoenzimàtica a Brassica campestris. Llicenciatura de Grau, University of Barcelona, Spain

    Google Scholar 

  • Vallejos CE (1983) Enzyme activity staining. In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, part A. Elsevier, Amsterdam, Oxford, New York, pp 469–516

    Google Scholar 

  • Venkasterwarlu J, Kamala T (1971) Pachytene chromosome complements and genome analysis in Brassica. J Indian Bot Soc 50:442–449

    Google Scholar 

  • Zamir D, Tadmor Y (1986) Unequal segregation of nuclear genes in plants. Bot Gaz 147:355–358

    Article  Google Scholar 

Download references

Author information

Author notes

  1. M. J. Truco

    Present address: Department of Biology, Utah State University, 84322-5305, Logan, Utah, USA

Authors and Affiliations

  1. Department of vegetable Crops, University of California, 95616, Davis, CA, USA

    M. J. Truco & C. F. Quiros

Authors
  1. M. J. Truco
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. F. Quiros
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by J. S. Beckmann

Rights and permissions

Reprints and permissions

About this article

Cite this article

Truco, M.J., Quiros, C.F. Structure and organization of the B genome based on a linkage map in Brassica nigra . Theoret. Appl. Genetics 89, 590–598 (1994). https://doi.org/10.1007/BF00222453

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00222453

Key words

Search

Navigation