Abstract
The transmission of a structurally-hypervariable fraction of the mitochondrial genome has been studied in 42 F1 progenies obtained from reciprocal crosses between self-pollinated alloplasmic wheat plants regenerated after long-term somatic embryogenesis. This fraction of the genome is maternally and stoichiometrically inherited. In contrast, some additional restriction fragments specific to regenerated plants display a more complex mode of sexual transmission: one of the additional fragments was stoichiometrically and systematically inherited whereas two others were detected only in certain F1 hybrids. Assuming that the detection, by Southern analysis, of such a fragment in regenerated plants is due to the amplification of a pre-existing substoichiometric molecule generated by the activation of a rare recombination event, our results suggest that the probability of detecting a novel fragment in the F1 hybrids could be determined by the length of the repeated sequence at which recombination occurs.
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References
André C, Levy A, Walbot V (1992) Small repeated sequences and the structure of plant mitochondrial genomes. Trends Genet 8: 128–132
Aubry C (1990) Doctoral thesis, Orsay, France
Bowman CM, Koller B, Delius H, Dyer TA (1981) A physical map of wheat chloroplast DNA showing the location of the structural genes for the ribosomal RNAs and the large subunit of the ribulose-1,5-biphosphate carboxylase. Mol Gen Genet 183: 93–101
Brears T, Curtis GJ, Lonsdale DM (1989) A specific rearrangement of mitochondrial DNA induced by tissue culture. Theor Appl Genet 77: 620–624
Brown PTH (1991) The spectrum of molecular changes associated with somaclonal variation. IAPTC Newslett 66: 14–25
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1: 19–21
Dennis, ES, Brettell RIS, Peacock WJ (1987) A tissue culture-induced Adh1 null mutant of maize results from a single base change. Mol Gen Genet 210: 181–183
Dörfel P, Weihe A, Knösche R, Börner T (1989) Mitochondrial DNA of Chenopodium album (L.): a comparison of leaves and suspension cultures. Curr Genet 16: 375–380
Fairbanks DJ, Smith SE, Brown JK (1988) Inheritance of large mitochondrial RNAs in alfalfa. Theor Appl Genet 76: 619–622
Erickson L, Kemble R (1990) Paternal inheritance of mitochondria in rapeseed (Brassica napus). Mol Gen Genet 222: 135–139
Gengenbach BG, Green CE, Donovan CM (1977) Inheritance of selected pathotoxin resistance in maïze plants regenerated from cell cultures. Proc Natl Acad Sci USA 74: 5113–5117
Giles RE, Blanc M, Cann HM, Wallace DC (1980) Maternal inheritance of mitochondrial DNA. Proc Natl Acad Sci USA 77: 6715–6719
Grayburn WS, Bendich AJ (1987) Variable abundance of a mitochondrial DNA fragment in cultured tobacco cells. Curr Genet 12: 257–261
Hartmann C, De Buyser J, Henry Y, Falconet D, Lejeune B, Benslimane AA, Quétier F, Rode A (1987) Time-course of mitochondrial genome variation in wheat embryogenic somatic tissue cultures. Plant Sci 53: 191–198
Hartmann C, Henry Y, De Buyser J. Aubry C, Rode A (1989) Identification of new mitochondrial genome organizations in wheat plants regenerated from somatic tissue cultures. Theor Appl Genet 77: 169–175
Hartmann, C, De Buyser J, Henry Y, Morére-Le Paven MC, Dyer T, Rode A (1992) Nuclear genes control changes in the organization of the mitochondrial genome in tissue cultures derived from immature embryos of wheat. Curr Genet 21: 515–520
Hartmann C, Récipon H, Jubier MF, Valon C, Delcher-Besin E, Henry Y, De Buyser J, Lejeune B, Rode A (1994) Mitochondrial DNA variability detected in a single wheat regenerant involves a rare recombination event across short repeat. Curr Genet 25: 456–464
Hutchison III CA, Newbold JE, Potter SS, Edgell MH (1974) Maternal inheritance of mammalian mitochondrial DNA. Nature 251: 536–538
Karp A (1992) On the current understanding of somaclonal variation. Oxford Surveys Pl Mol Cell Biol 7: 1–58
Lee M, Phillips RL (1988) The chromosomal basis of somaclonal variation. Annu Rev Plant Physiol Plant Mol Biol 39: 413–437
Lonsdale DM, Hodge TP, Fauron C (1984) The physical map and organization of the mitochondrial genome from the fertile cytoplasm of maize. Nucleic Acids Res 12: 9249–9261
McNay JW, Chourey PS, Pring DR (1984) Molecular analysis of genomic stability of mitochondrial DNA in tissue cultured cells of maize. Theor Appl Genet 67: 433–437
Morgens PH, Grabau EA, Gesteland RF (1984) A novel soybean mitochondrial transcript resulting from a DNA rearrangement involving the 5s rRNA gene. Nucleic Acids Res 12: 5665–5684
Neale DB, Marshall KA, Sederoff RR (1989) Chloroplast and mitochondrial DNA are paternally inherited in Sequoia sempervirens D. Don Endl. Proc Natl Acad Sci USA 86: 9347–9349
Newton KJ (1988) Plant mitochondrial genomes: organization, expression and variation. Annu Rev Plant Physiol Plant Mol Biol 39: 503–532
Palmer JD, Shields CR (1984) Tripartite structure of the Brassica campestris mitochondrial genome. Nature 307: 437–440
Peschke VM, Phillips RL, Gengenbach GG (1987) Discovery of transposable element activity among progeny of tissue culturederived maize plants. Science 238: 804–807
Quétier F, Lejeune B, Delorme S, Falconet D, Jubier MF (1985) Molecular form and function of the wheat mitochondrial genome. In: Groot G, Hall T (eds) Molecular form and function of plant genomes. Plenum Press, New York, pp 413–420
Rode A, Hartmann C, Falconet D, Lejeune B, Quétier F, Benslimane AA, Henry Y, De Buyser J (1987) Extensive mitochondrial DNA variation in somatic tissue cultures initiated from wheat immature embryos. Curr Genet 12: 369–376
Rode A, Hartmann C, De Buyser J, Henry Y (1988) Evidence for a direct relationship between mitochondrial genome organization and regeneration ability in hexaploid wheat somatic tissue cultures. Curr Genet 14: 387–394
Saleh NM, Gupta HS, Finch RP, Cocking EC, Mulligan BJ (1990) Stability of mitochondrial DNA in tissue-cultured cells of rice. Theor Appl Genet 79: 342–346
Schmitz UK (1988) Molecular analysis of mitochondrial DNA and its inheritance in Epilobium. Curr Genet 13: 411–415
Shirzadegan M, Christey M, Earle ED, Palmer JD (1989) Rearrangement, amplification, and assortment of mitochondrial DNA molecules in cultured cells of Brassica campestris. Theor Appl Genet 77: 17–25
Shirzadegan M, Palmer JD, Christey M, Earle E (1991) Patterns of mitochondrial DNA instability in Brassica campestris cultured cells. Plant Mol Biol 16: 21–37
Vedel F, Quétier F, Cauderon Y, Dosba F, Doussinault G (1981) Studies on maternal inheritance in polyploid wheats with cytoplasmic DNAs as genetic markers. Theor Appl Genet 59: 239–246
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Communicated by D. R. Pring
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Morère-Le Paven, M.C., De Buyser, J., Henry, Y. et al. Unusual inheritance of the mitochondrial genome organization in the progeny of reciprocal crosses between alloplasmic hexaploid wheat regenerants. Theoret. Appl. Genetics 89, 572–576 (1994). https://doi.org/10.1007/BF00222450
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DOI: https://doi.org/10.1007/BF00222450