Abstract
While anther culture or pollinations with Hordeum bulbosum have provided suitable methods for haploid production in bread wheat, they have been largely unsuccessful in durum wheat. Pollinations with maize were used in an attempt to produce haploid seedlings and, from these, fertile doubled haploids of durum wheats. Moreover, the effect of various concentrations and combinations of a synthetic auxin, 2, 4-dichlorophenoxyacetic acid (2,4-D), kinetin, and an ethylene inhibitor, silver nitrate (AgNO3), on embryo recovery were also investigated. Haploid seedlings were recovered from Triticum turgidum ssp. turgidum cv ‘Rampton Rivet’ pollinated with maize following in-vivo treatment of ovaries with 2,4-D for 2 weeks and subsequent embryo culture. The recovery of haploid seedlings from T. turgidum ssp. durum cv. ‘Wakona’ pollinated with maize necessitated the addition of AgNO3, to the 2,4-D treatment. Overall, haploid seedlings were produced in 1.7% and 3.3% of pollinated florets for “Rampton Rivet” and “Wakona” respectively. The success of the present work represents a significant breakthrough for haploid production in durum wheats. Wide hybridization with maize followed by in-vivo treatment of ovaries with 2,4-D alone, or in combination with AgNO3, may provide a widely-applicable method of haploid production in tetraploid wheats.
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References
Abeles FB (1973) Ethylene in plant biology. Academic Press, New York
Bennici A (1986) Durum wheat (Triticum durum Desf.) In: Bajaj YPS (ed) Biotechnology in agriculture and forestry vol 2. Crops. Springer Verlag, Berlin, pp 89–104
Beyer EM (1976) A potent inhibitor of ethylene action in plants. Plant Physiol 58:268–271
Brewbaker JL, Emery GC (1962) Pollen radiobotany. Radiat Bot 1:101–154
Britten EJ (1950) Natural and induced parthenocarpy in maize and its relation to hormone production by the developing seed. Am J Bot 37:345–352
Comeau A, Plourde A, St Pierre CA, Nadeau P (1988) Production of doubled haploid wheat lines by wheat x maize hybridization (abstract). Genome 30, Suppl 1:482
Davies KM, Hobson GE, Grierson D (1988) Silver ions inhibit the ethylene-stimulated production of ripening related mRNAs in tomato. Plant Cell Environ 11:729–738
Hadwiger MA, Heberle-Bors E (1986) Pollen plant production in Triticum turgidum ssp. durum. In: Horn W, Jensen CJ, Odenbach W, Schieder O (eds) Genetic manipulation in plant breeding. Proc Int Symp organized by EUCARPIA, 1985. Walter de Gruyter, Berlin, pp 303–305
Hsam SLK, Zeller FJ (1993) Haploid production in durum wheat by the interaction of Aegilops kotschyi cytoplasm and the 1BL/1RS chromosomal interchange. Theor Appl Genet 86:951–954
Inagaki M, Tahir M (1990) Comparison of haploid production frequencies in wheat varieties crossed with Hordeum bulbosum L. and maize. Japan J Breed 40:209–216
Sahara H, Tsunewaki K (1960) Production of polyploid wheat by nitrous oxide. Proc Jpn Acad 36:658–668
Laurie DA (1989) Factors affecting fertilization frequency in crosses of Triticum aestivum cv “Highbury” x Zea mays cv “Seneca 60”. Plant Breed 103:133–140
Laurie DA, Bennett MD (1986) Wheat x maize hybridization. Can J Genet Cytol 28:313–316
Laurie DA, Bennett MD (1987) The effect of the crossability loci Kr1 and Kr2 on fertilization frequency in hexaploid wheat x maize crosses. Theor Appl Genet 73:403–409
Laurie DA, Bennett MD (1988a) The production of haploid wheat plants from wheat x maize crosses. Theor Appl Genet 70:100–105
Laurie DA, Bennett MD (1988b) Chromosome behaviour in wheat x maize, wheat x sorghum and barley x maize crosses. In: Brandham PE (ed) Kew Chromosome Conference III, proceedings of the third chromosome conference 1987. Her Majesty's Stationary Office, London, pp 167–177
Laurie DA, O'Donoughue LS, Bennett MD (1990) Wheat x maize and other sexual wide-hybrids: their potential for genetic manipulation and crop improvement. In: Gustafson JP (ed) Gene manipulation in plant improvement II. Plenum Press, New York, pp 400–500
Marshall DR, Molnàr-Làng M, Ellison FW (1983) Effects of 2.4-D on parthenocarpy and cross-compatibility in wheat. Cereal Res Commun 11:213–218
Mathias RJ, Boyd LA (1988) The growth in culture of detached ovaries of wheat (Triticum aestivum L. em. Thell.) Plant Breed 100:143–146
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Norstog KJ (1973) New synthetic medium for the culture of premature barley embryos. In Vitro 8:307–308
O'Donoughue LS (1990) Chromosome behaviour and reproductive physiology in cereal wide-crosses, PhD thesis, Cambridge University, UK
O'Donoughue LS, Bennett MD (1994) Comparative responses of tetraploid wheats pollinated with Zea mays L. and Hordeum bulbosum L. Theor Appl Genet 87:673–680
Pienaar R de V, van Niekerk HA (1973) The effect of gamma-irradiation on wheat gametophytes. In: Sears ER, Sears LMS (eds) Proc 4th Int Wheat Genet Symp. University of Missouri, Columbia, pp 289–294
Riera-Lizarazu O, Mujeeb-Kazi A (1990) Maize (Zea mays L.)- mediated wheat (Triticum aestivum L.) polyhaploid production using various crossing methods. Cereal Res Commun 18:339–345
Rogers OM (1973) Growth regulator induction of parthenocarpy in maize. Maize Genet Coop Newslett 47:157–159
Romani RJ, Hess BM, Leslie CA (1989) Salicylic acid inhibition of ethylene production by apple discs and other plant tissues. J Plant Growth Regul 8:63–69
Salisbury FB, Ross CW (1978) Plant physiology, 2nd edn. Wadsworth Publishing Company, Belmont
Sharma GC, Wang WC, Sapra VT (1982) Effect of genotype, media and temperature pretreatment on callus initiation in triticale, wheat and rye anther culture. Cereal Res Commun 10:143–150
Sitch LA, Snape JW (1986) Doubled haploid production in winter wheat and triticale genotypes. Euphytica 35:1051–1054
Snape JW (1989) Doubled haploid breeding: theoretical basis and practical applications. In: Mujeeb-Kazi A, Sitch LA (eds) Review of advances in plant biotechnology 1985–88. 2nd Int Symp on genetic manipulation in crops. CIMMYT and IRRI, Mexico and Manila, pp 19–31
Snape JW, Simpson E (1981) Uses of doubled haploid lines for genetical analysis. In: Asher et al. (eds) Barley genetics IV. Proc 4th Int Barley Genet Symp. Edinburgh University Press, Edinburgh, pp 704–709
Snape JW, Chapman V, Moss J, Blanchard CE, Miller TE (1979) The crossabilities of wheat varieties with Hordeum bulbosum. Heredity 42:291–298
Suenaga K, Nakajima K (1989) Efficient production of haploid wheat (Triticum aestivum) through crosses between Japanese wheat and maize (Zea mays). Plant Cell Rep 8:263–266
Zhu ZQ, Wang JJ, Sun JS (1980) The induction of albino pollen plants and preliminary observation of their ploidy in Triticum durum Desf. In: Davies DR, Hopwood DA (eds) The plant genome. 4th John Innes Symp and 2nd Int Haploid Conf. The John Innes Charity, Norwich, pp 254–255
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Communicated by K. Tsunewaki
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O'Donoughue, L.S., Bennett, M.D. Durum wheat haploid production using maize wide-crossing. Theoret. Appl. Genetics 89, 559–566 (1994). https://doi.org/10.1007/BF00222448
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DOI: https://doi.org/10.1007/BF00222448