Abstract
The cytological possibility of gene transfer from Sinapis pubescens to Brassica napus was investigated. Intergeneric hybrids between Brassica napus (2n = 38) and Sinapis pubescens (2n = 18) were produced through ovary culture. The F1 hybrids were dihaploid and the chromosome configurations were (0–1) III + (2–11) II + (5–24) I . One F2 plant with 38 chromosomes was obtained from open pollination of the F1 hybrid. Thirty-one seeds were obtained from the backcross of the F2 plant with B. napus. Five out of seven plants had 38 chromosomes, and the pollen stainability ranged from 0% to 81.4%. In the B2 plants obtained from the backcross of B1 plants with B. napus, 66.7% of the plants examined had 38 chromosomes. S. pubescens may become a gene source for the improvement of B. napus.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Attia T, Röbbelen G (1986) Meiotic pairing in haploids and amphidiploids of spontaneous versus synthetic origin in rape, Brassica napus L. Can J Genet Cytol 28:330–334
Bjaj YPS, Mahajan SK, Labana KS (1986) Interspecific hybridization of Brassica napus and B. juncea through ovary, ovule and embryo culture. Euphytica 35:103–109
Chiang BY, Grant WF, Chiang MS (1978) Transfer of resistance to race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. oleracea var ‘capitata’). II. Meiosis in the interspecific hybrids between 2x and 4x cabbage. Euphytica 27:81–93
Chiang BY, Chiang MS, Grant WF, Crete R (1980) Transfer of resistance to race 2 of Plasmodiophora brassicae from Brassica napus to cabbage (B. oleracea ssp. capitata). VI. A resistant 18-chromosome b1 plant and its B2 progenies. Euphytica 29:47–55
Gundimeda HR, Prakash S, Shivanna KR (1992) Intergeneric hybrids between Enarthrocarpus lyratus, a wild species, and crop brassicas. Theor Appl Genet 83:655–662
Harberd DJ, McArthur ED (1980) Meiotic analysis of some species and genus hybrids in the Brassiceae. In: Tsunoda S, Hinata K, Gómez-Campo C (eds) Brassica crops and wild allies. Japan Scientific Societies Press, Tokyo, pp 65–87
Inomata N (1977) Production of interspecific hybrids between Brassica campestris and Brassica oleracea by culture in vitro of excised ovaries. I. Effects of yeast extract and case in hydrolysate on the development of excised ovaries. Jpn J Breed 27:295–304
Inomata N (1980) Hybrid progenies of the cross, Brassica campestris x B. oleracea. I. Cytological studies on F1 hybrids. Jpn J Genet 55:189–202
Inomata N (1983) Hybrid progenies of the cross, Brassica campestris x B. oleracea. II. Crossing ability of F1 hybrids and their progenies. Jpn J Genet 58:433–449
Inomata N (1985a) A revised medium for in vitro culture of Brassica ovaries. In: Chapman GP, Mantell SH, Daniels RW (eds) The experimental manipulation of ovule tissues. Longman. London, pp 164–176
Inomata N (1985b) Interspecific hybrids between Brassica campestris and B. cretica by ovary culture in vitro. Cruciferae Newsl 10:92–93
Inomata N (1986) Interspecific hybrids between Brassica campestris and B. bourgeaui by ovary culture in vitro. Cruciferae Newsl 11:14–15
Inomata N (1987) Interspecific hybrids between Brassica campestris and B. montana by ovary culture in vitro. Cruciferae Newsl 12:8–9
Inomata N (1990) Interspecific hybridization in Brassica through ovary culture. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 10: legumes and oilseed crops I. Springer, Berlin Heidelberg New York Tokyo, pp 367–384
Inomata N (1991a) Hybrid progenies of the cross, Brassica campestris x B. oleracea. IV. Crossability of F2, B1 and hybrid plants, and their progenies. Jpn J Genet 66:449–460.
Inomata N (1991b) Intergeneric hybridization in Brassica juncea x Sinapis pubescens and B. napus x S. pubescens, and their cytological studies. Cruciferae Newsl 14/15:10–11
Inomata N (1992) Intergeneric hybridization between Brassica carinata and Sinapis turgida through ovary culture, and cytology and crossability of their progenies. Genet (Life Sci Adv) 11:129–140
Inomata N (1993a) Embryo rescue technique for wide hybridization. In: Labana KS, Banga SS, Banga SK (eds) Breeding oilseed brassicas. Springer, Berlin Heidelberg New York Tokyo, pp 94–107
Inomata N (1993b) Crossability and cytology of hybrid progenies in the cross between Brassica campestris and three wild relatives of B. oleracea, B. bourgeaui, B. cretica and B. montana. Euphytica 69:7–17
Lelivelt CLC, Leunissen EHM, Frederiks HJ, Helsper JPFG, Krens FA (1993) Transfer of resistance to the beet cyst nematode (Heterodera schachtii Schm.) from Sinapis alba L. (white mustard) to the Brassica napus L. gene pool by means of sexual and somatic hybridization. Theor Appl Genet 85:688–696
Mathias R (1991) Improved embryo rescue technique for intergeneric hybridization between Sinapis species and Brassica napus. Cruciferae Newsl 14/15:90–91
McNaughton H, Ross CL (1978) Interspecific and intergeneric hybridization in the Brassica with special emphasis on the improvement of forage crops. Annu Rep Scott Plant Breed Stn 75–110
Mizushima U (1952) Karyogenetic studies on Brassiceae. Gihodo, Tokyo, p 112
Mohapatra D, Bajaj YPS (1987) Interspecific hybridization in Brassica juncea x Brassica hirta using embryo rescue. Euphytica 36:321–326
Nanda Kumar PBA, Shivanna KR (1993) Intergeneric hybridization between Diplotaxis siettiana and crop brassicas for the production of alloplasmic lines. Theor Appl Genet 85:770–776
Takahata Y (1990) Production of intergeneric hybrids between a C3-C4 intermediate species Moricandia arvensis and a C3 species Brassica oleracea through ovary culture. Euphytica 46:259–264
Takahata Y, Takeda T (1990) Intergeneric (intersubtribe) hybridization between Moricandia arvensis and Brassica A and B genome species by ovary culture. Theor Appl Genet 80:38–42
Yadav RC, Sareen PK, Chowdhury JB (1991) Interspecific hybridization in Brassica juncea x Brassica tournefortii using ovary culture. Cruciferae Newsl 14/15:84
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Inomata, N. Intergeneric hybridization between Brassica napus and Sinapis pubescens, and the cytology and crossability of their progenies. Theoret. Appl. Genetics 89, 540–544 (1994). https://doi.org/10.1007/BF00222445
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00222445