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Organ culture of adult Rat and mouse tracheal epithelium: I. Ultrastructure following various culture periods

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Summary

Electron microscopic studies of adult rat and mouse tracheal epithelium maintained in organ culture for a period of up to 6 days were performed. In specimens cultured for 60 minutes no conspicuous micromorphological alterations could be observed. Following culture periods from 1–6 days the number of cilia in some of the ciliated cells was reduced while their structure and the other ultrastructural details of the epithelial cells were preserved. In specimens cultured for 5–6 days some additional alterations could be noticed: polymorphism of mitochondria, increased number of lysosomes, appearance of intracellular vacuoles, exhaustion of goblet cells and disappearance of granulated mast-cell like cells in the rat tracheal epithelium.

I want to thank Miss J. Selbmann and Mrs. S. Kolassa for technical help and Mr. H. Wagner for preparing the micrographs; I am indebted to Dr. D. Kerjaschki and to Mr. H. Hörandner for performing preparations for scanning electron microscopy and to Mr. P. Scholze (Österreichische Studiengesellschaft für Atomenergie, Institut für Metallurgie, Abteilung Fremdkörperphysik) for preparing the scanning electron micrograph.

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Authors and Affiliations

  1. Department of Micromorphology and Electron Microscopy, University of Vienna, Vienna, Austria

    M. Pavelka

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  1. M. Pavelka
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This paper is dedicated to Prof. Bargmann on the occasion of his 70th birthday.

This work was supported by the Fond zur Förderung der wissenschaftlichen Forschung: Project 2099.

The author wishes to express her appreciation to Prof. Stockinger for suggestion and advice.

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Pavelka, M. Organ culture of adult Rat and mouse tracheal epithelium: I. Ultrastructure following various culture periods. Cell Tissue Res. 165, 371–382 (1976). https://doi.org/10.1007/BF00222440

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  • DOI: https://doi.org/10.1007/BF00222440

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