Summary
Insulin-like immunoreactivity can be localized to cells of the intestine in the-area of the hepatopancreas of Mytilus edulis L. No cross-reactivity can be obtained with anti-glucagon, anti-gastrin, anti-pentagastrin or anticaerulin. The cells containing the substance immunoreactive to mammalian anti-insulin, can be restained with paraldehyde-fuchsin.
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Collip, J.B.: The demonstration of an insulin-like substance in the tissue of the clam (Mya arenaria). J. biol. Chem. 55, 39 (1923)
Coons, A.H., Leduc, E.H., Connolly, J.M.: Studies on antibody production. 1. Method for the histochemical demonstration of specific antibody and its application to the study of the hyperim- mune rabbit. J. exp. Med. 102, 49–59 (1955)
Chan, V.G., Fontaine, A.R.: Is there a β-cell homologue in starfish? Gen. Comp. Endocr. 16, 183–191 (1971)
Davidson, J.K., Falkmer, S., Mehrotra, B.K., Wilson, S.: Insulin assays and light microscopical studies of digestive organs in protostomian and deuterostomian species and in coelenterates. Gen. comp. Endocr. 17, 388–401 (1971)
Falck, B.H., Hillarp, N.-Å., Thieme, G., Torp, A.: Fluorescence of catecholamines and related compounds condensed with formaldehyde. J.Histochem. Cytochem. 5, 188–195 (1962)
Falkmer, S.: Insulin production in vertebrates and invertebrates. Gen. comp. Endocr. 3, 184–191 (1972)
Falkmer, S., Emdin, S., Havu, N., Lundgren, G., Marques, M., Östberg, Y., Steiner, D.N., Thomas, N.W.: Insulin in invertebrates and cyclostomes. Amer. Zool. 13, 625 -638 (1973)
Falkmer, S., Wilson, S.: Comparative aspects of the immunology and biology of insulin. Diabetologia 3, 519–528 (1967)
Grimelius, L.: A silver nitrate stain for A2-cells in human pancreatic islets. Acta Soc. Med. upsalien. 73, 243–270 (1968)
Håkanson, R., Lilja, B., Owman, C.: Cellular localization of histamine and monoamines in the gastric mucosa of man. Histochemie 18, 74–86 (1969)
Noorden, S. Van, Greenberg, J., Pearse, A.G.E.: Cytochemical and immunofluorescence investigations on polypeptide hormone localization in the pancreas and gut of the larval lamprey. Gen. comp. Endocr. 19, 192–199 (1972)
Noorden, S. Van, Pearse, A.G.E.: Immunoreactive polypeptide hormones in the pancreas and gut of the lamprey. Gen. comp. Endocr. 23, 311–324 (1974)
Pearse, A.G.E.: Common cytochemical and ultrastructural characteristics of cells producing polypep- tide hormones (the APUD series) and their relevance to thyroid and ultimobranchial-C-cells and calcitonin. Proc. roy. Soc. B 170, 71–80 (1968)
Pearse, A.G.E.: The cytochemistry and ultrastructure of polypeptide hormone-producing cells of the APUD series and the embryologic, physiologic and pathologic implications of the concept. J. Histochem. Cytochem. 17, 303–313 (1969)
Pearse, A.G.E., Polak, J.M.: Bifunctional reagents as vapour and liquid phase fixatives for immunohis- tochemistry. Histochemie 7, 179–186 (1975)
Polak, J.M., Bussolati, G., Pearse, A.G.E.: Cytochemical, immunofluorescence and ultrastructural investigations on the antral G cells in hyperparathyroidism. Virchows Arch. Abt. 9, 187–197 (1971)
Ritzén, M., Hammerström, L., Ullberg, S.: Autoradiographic distribution of 5-hydroxytryptamine and 5-hydroxytryptophan in the mouse. Biochem. Pharmacol. 14, 313–321 (1965)
Solcia, E., Vassallo, G., Capella, C.: Selective staining of endocrine cells by basic dyes after acid hydrolysis. Stain Technol. 43, 257–263 (1968)
Solcia, E., Capella, C., Vassallo, G.: Lead haematoxylin as a stain for endocrine cells. Histochemie 20, 116–126 (1969)
Wilson, S., Falkmer, S.: Starfish insulin. Canad. J. Biochem. 43, 1615–1624 (1965)
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Dedicated to Prof. Dr. Drs. h.c. W. Bargmann at his 70th birthday.
I thank Prof. A.G.E. Pearse for the opportunity to carry out this work in the Department of Histochemistry, Royal Postgraduate Medical School, London, England. The work was carried out with the aid of a grant from the Studienstiftung des Deutschen Volkes e.V., Germany (FRG).
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Fritsch, H.A.R., Van Noorden, S. & Pearse, A.G.E. Cytochemical and immunofluorescence investigations on insulin-like producing cells in the intestine of Mytilus edulis L. (Bivalvia). Cell Tissue Res. 165, 365–369 (1976). https://doi.org/10.1007/BF00222439
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DOI: https://doi.org/10.1007/BF00222439