Summary
Ecdysone was demonstrated by ultrastructural immunocytochemistry to be present in the mitochondria of the Y-organs of the crayfish Orconectes limosus. This is in remarkable contrast to the prothoracic glands of insects and suggests substantial differences in the biosynthesis of the same hormone, ecdysone, in crustaceans and insects.
References
Birkenbeil H (1983) Ultrastructural and immunocytochemical investigation of ecdysteroid secretion by the prothoracic gland of the waxmoth Galleria mellonella. Cell Tissue Res 229:433–441
Birkenbeil H, Eckert M (1982) Untersuchungen zur Methodik des ultrastrukturellen immunocytochemischen Nachweises von Ecdysteroiden. Acta Histochem 71:133–143
Birkenbeil H, Gersch M (1979) Ultrastructure of the Y-organ of Astacus astacus (L.) (Crustacea) in relation of the moult cycle. Cell Tissue Res 196:519–524
Eibisch H, Gersch M, Eckert M, Böhm G-A (1980) Investigations of the ecdysteroid production in the prothoracic glands and of the ecdysteroid level in the hemolymph of Periplaneta americana larvae by means of radioimmunoassay (RIA) with an anti-ecdysterone serum. Zool Jb Physiol 84:153–163
Gronemeyer H, Pongs O (1980) Localization of ecdysterone on polytene chromosomes of Drosophila melanogaster. Proc Natl Acad Sci USA 77:2108–2112
Mitani F, Shimizu T, Ueno R, Ishimura Y, Izumi S, Komatsu N, Watanabe K (1982) Cytochrome P-450 1l β and P-450 sec in adrenal cortex: Zonal distribution and intramitochondrial localization by the horseradish peroxidase-labeled antibody method. J Histochem Cytochem 30:1066–1074
Spindler K-D, Keller R, O'Connor JD (1980) The role of ecdysteroids in the Crustacean molting cycle. In: Hoffmann JA (ed) Progress in ecdysone research. Elsevier, North-Holland, Biomedical Press, pp 247–280
Tamaoki B (1973) Steroidogenesis and cell structure. J Steroid Biochem 4:89–118
Author information
Authors and Affiliations
Additional information
Supported by Sächsische Akademie der Wissenschaften zu Leipzig
The authors are indebted for excellent assistance to Mrs. Angelika Schmidt
Rights and permissions
About this article
Cite this article
Birkenbeil, H., Eckert, M. Immuno-electron-microscopic evidence of ecdysteroids in Y-organ of Orconectes limosus (Crustacea, Decapoda). Cell Tissue Res. 233, 233–236 (1983). https://doi.org/10.1007/BF00222247
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00222247