Summary
When the resorption zones of the distal femoral and proximal tibial epiphyseal plates of rats are examined with the electron microscope, numerous macrophages can be seen surrounding degenerated chondrocytes. Macrophages are also found in the neighbourhood of the invading sinusoids and in close proximity to multinucleate osteoclasts. Cell fusion, however, could not be observed. Repeated transfusions of female leukocytes to isohistogeneic growing male rats caused an increase in the number of nuclei containing a Barr body in the osteoclasts of the males. The result suggests that leukocytes — of which only the monocytes come in question — are the source of multinucleate osteoclasts in endochondral ossification.
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References
Allyn PG, Minkin C (1976) Origin of the osteoclast: studies with a quail-mouse chimera. J Dent Res 55 B:219
Andersen H, Matthiessen ME (1966) The histiocyte in human fetal tissues: its morphology, cytochemistry, origin, function, and fate. Z Zellforsch 72:193–211
Anderson CE, Parker J (1966) Invasion and resorption in enchondral ossification. An electron microscopic study. J Bone Joint Surg 48 A: 899–914
Ash P, Loutit JF, Townsend KMS (1980) Osteoclasts derived from haemopoietic stem cells. Nature 283:669–670
Castro N de, Silva Sasso W da, Goes MR de (1956) Sex determination in rats (Rattus norvegicus var. albinus) through the ameloblasts of the dental germ. Nature 178:1059–1060
Chambers TJ (1979) Phagocytosis and trypsin-resistant glass adhesion by osteoclasts in culture. J Pathol 127:55–60
Coccia PF, Krivit W, Cervenka J, Clawson C, Kersey JH, Kim TH, Nesbit ME, Ramsey NKC, Warkentin PI, Teitelbaum SL, Kahn AJ, Brown DM (1980) Successful bone-marrow transplantation for infantile malignant osteopetrosis. New Engl J Med 302:701–708
Fischman DA, Hay ED (1962) Origin of osteoclasts from mononuclear leukocytes in regenerating newt limbs. Anat Rec 143:329–337
Göthlin G, Ericsson JLE (1976) The osteoclast. Review of ultrastructure, origin and structurefunction relationship. Clin Orthop 120:201–231
Hall BK (1975) The origin and fate of osteoclasts. Anat Rec 183:1–13
Haythorn SR (1929) Multinucleated giant cells with particular reference to the foreign body giant cell. Arch Pathol Lab Med 7:651–713
Hinrichsen K, Gothe HD (1958) Morphologische und statistische Untersuchungen an Zellkernen von Ratten und Mäusen zur Frage einer cytologischen Geschlechtsdiagnostik. Z Zellforsch 48:429–449
Jee WSS, Nolan PD (1963) Origin of osteoclasts from the fusion of phagocytes. Nature 200:225–226
Jones SJ, Hogg NM, Shapiro IM, Slusarenko M, Boyde A (1981) Cells with Fc receptors in the cell layer next to osteoblasts and osteoclasts on bone. Metabolic Bone Dis Relat Res 2:357–362
Jotereau FV, Le Douarin NM (1978) The developmental relationship between osteocytes and osteoclasts: a study using the quail-chick nuclear marker in endochondral ossification. Dev Biol 63:253–265
Kahn AJ, Simmons DJ, Krukowski M (1981) Osteoclast precursor cells are present in the blood of preossification chick embryos. Dev Biol 84:230–234
Kember NF (1960) Cell division in endochondral ossification. A study of cell proliferation in rat bones by the method of tritiated thymidine autoradiography. J Bone Joint Surg 428:824–839
Kimmel DB, Jee WSS (1980) Bone cell kinetics during longitudinal bone growth in the rat. Calcif Tissue Int 32:123–133
Knese KH, Knoop AM (1961) Elektronenmikroskopische Beobachtungen über die Zellen in der Eröffnungszone des Epiphysenknorpels. Z Zellforsch 54:1–38
Ko JS, Bernard GW (1981) Osteoclast formation in vitro from bone marrow mononuclear cells in osteoclast-free bone. Am J Anat 161: 415–425
Kölliker A (1873) Die normale Resorption des Knochengewebes und ihre Bedeutung für die Entstehung der typischen Knochenformen. Vogel, Leipzig
Leder LD (1967) Der Blutmonocyt. Exp Med Pathol Klin 23:1–293
Mariano M, Spector WG (1974) The formation and properties of macrophage polykaryons (inflammatory giant cells). J Pathol 113:1–19
Marks SC, Walker DG (1981) The hematogenous origin of osteoclasts: Experimental evidence from osteopetrotic (microphthalmic) mice treated with spleen cells from beige mouse donors. Am J Anat 161:1–10
Owen M (1970) The origin of bone cells. Int Rev Cytol 28:213–238
Rhee HJ van der, Burgh-de Winter CPM van der, Daems WTh (1979a) The differentiation of monocytes into macrophages, epitheloid cells, and multinucleated giant cells in subcutaneous granulomas. I. Fine structure. Cell Tissue Res 197:355–379
Rhee HJ van der, Burgh-de Winter CPM van der, Daems WTh (1979b) The differentiation of monocytes into macrophages, epithelioid cells, and multinucleated giant cells in subcutaneous granulomas. II. Peroxidatic activity. Cell Tissue Res 197:379–396
Rohr H (1964) Zur Entstehung der mehrkernigen Osteoklasten nach Parathormongabe. Autoradiographische Untersuchungen mit H3-Thymidin. Klin Wschr 42:1209–1212
Schenk RK, Spiro D, Wiener J (1967) Cartilage resorption in the tibial epiphyseal plate of growing rats. J Cell Biol 34:275–291
Schmidt D (1974) Blutvolumen. Johann Ambrosius Barth, Leipzig
Scott BL (1967) Thymidine-3H electron microscope radiography of osteogenic cells in the fetal rat. J Cell Biol 35:115–126
Testa NG, Allen TD, Lajtha LG, Onions D, Jarret O (1981) Generation of osteoclasts in vitro. J Cell Sci 47:127–137
Tinkler SMB, Williams DM, Johnson NW (1981 a) Osteoclast formation in response to intraperitoneal injection of 1α-hydroxycholecalciferol in mice. J Anat 133:91–97
Tinkler SMB, Linder JE, Williams DM, Johnson NW (1981b) Formation of osteoclasts from blood monocytes during 1α-OH Vit D-stimulated bone resorption in mice. J Anat 133:389–396
Tonna EA (1960) Osteoclasts and the aging skeleton: a cytological, cytochemical and autoradiographic study. Anat Rec 137: 251–270
Tonna EA (1963) Reply to Jee WSS and Nolan PD (1963). Nature 200:226–227
Walker DG (1973) Osteopetrosis cured by temporary parabiosis. Science 180:875
Young RW (1962) Cell proliferation and specialization during endochondral osteogenesis in young rats. J Cell Biol 14:357–370
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Stanka, P., Bargsten, G. Experimental study on the haematogenous origin of multinucleate osteoclasts in the rat. Cell Tissue Res. 233, 125–132 (1983). https://doi.org/10.1007/BF00222237
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DOI: https://doi.org/10.1007/BF00222237