Summary
The development of the rat lung is a process of continuing morphological change. Indications from work in other mammalian systems suggest that fibronectin may be important in the control of this process. The present study has examined embryonic, neonatal, and adult lung tissue of the rat by means of the peroxidase-antiperoxidase (PAP) technique to demonstrate fibronectin at the light-microscopic level. Positive reaction was observed with anti-fibronectin serum in all stages examined. Control sections treated with pre-immune serum or no primary serum gave negative results in each case. Fibronectin in adult tissue was localized to the alveolar surface and alveolar basal lamina. Neonatal tissue showed fibronectin on pulmonary tubule walls and in basal lamina while embryonic tissue revealed localization of the protein in the basal lamina and in association with small groups of cells at the base of septal buds. These findings suggest a role for fibronectin in the control of rat lung development. The results are discussed in terms of the known functions of fibronectin as a preliminary matrix for the subsequent deposition of collagenous connective tissue, as a cellular adhesion protein, and as surface-bound material for cellular migration.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adamson IYR, Bowden DH (1975) Derivation of type I epithelium from type II cells in the developing rat lung. Lab Invest 32:736–45
Alescio T (1973) Effect of a proline analogue, azetidine-2-carboxylic acid, on the morphogenesis in vitro of mouse embryonic lung. J Embryol Exp Morphol 29:439–443
Bornstein P, Duskin D, Balian G, Davidson JM, Crouch E (1978) Organization of extracellular proteins on the connective tissue cell surface: Relevance to cell-matrix interactions in vitro and in vivo. Ann NY Acad Sci 312:93–105
Bradley KH, McConnell SD, Crystal RG (1974) Lung collagen composition and synthesis: Characterization and changes with age. J Biol Chem 249:267–268
Bray BA, Mandl I, Turine GM (1980) Lung tissue fibronectin. Fed Proc 39: ABX # 993
Burri PM, Weibel ER (1977) In: Hodson WA (ed) Development of the lung. Marcel Dekker, New York, pp 215–268
Burri PM, Dbaly J, Weibel ER (1974) The postnatal growth of the rat lung: I. Morphometry. Anat Rec 178:711–730
Chiquet M, Puri EC, Turner DL (1979) Fibronectin mediates attachment of chicken myoblasts to a gelatin coated substratum. J Biol Chem 254:5475–5482
Couchman JR, Gibson WT, Thom D, Weaver AC, Rees DA, Parish WE (1979) Fibronectin distribution in epithelial and associated tissues of the rat. Arch Dermatol Res 266:295–310
Courtoy PJ, Kanwar YS, Hynes RO, Farquhar MG (1980) Fibronectin localization in the rat glomerulus. J Cell Biol 87:691–696
Greenberg JH, Seppa S, Seppa H, Tyl-Hewitt A (1981) Role of collagen and fibronectin in neural crest cell adhesion and migration. Dev Biol 87:259–266
Grotendorst GR, Seppa GE, Kleinman HK, Martin GR (1981) Attachment of smooth muscle cells to collagen and their migration toward platelet-derived growth factor. Proc Natl Acad Sci US 78:3669–3672
Hance AJ, Crystal RG (1975) The connective tissue of the lung. Am Rev Respir Dis 112:657–65
Hirai KT (1968) Specific affinity of oxidized amine dye (radical intermediate) for heme enzymes: Study in light and electron microscopy and spectrophotometrically, Acta Histochem (Jena) Cytochem 1:54–68
Hynes RO (1982) Fibronectin and its relation to cellular structure and behavior. In: Hay ED (ed) Cell biology of extracellular matrix. Plenum Press New York, pp 295–327
Kavinsky CJ, Garber BB (1979) Fibronectin associated with the glial component of embryonic brain cell cultures. J Supramol Struct 11:269–281
Kurkinen M, Alitalo K, Vaheri A, Stenman S, Saxen L (1979) Fibronectin in the development of the embryonic chick eye. Dev Biol 69:589–600
Kurkinen M, Vaheri A, Roberts PJ, Stenman S (1980) Sequential Appearance of Fibronectin and Collagen in Experimental Granulation Tissue. Lab Invest 43:47–51
Ledger PW, Uchida N, Tanzer ML (1980) Immunocytochemical localization of procollagen and fibronectin in human fibroblasts: Effects of the monovalent ionophore, monensin. J Cell Biol 87:663–671
Linder E, Stenman S, Lehto VP, Vaheri A (1978) Distribution of fibronectin in human tissues and relationship to other connective tissue components. Ann NY Acad Sci 312:151–159
Madri JA, Roll FJ, Furthmayer H, Foidart JM (1980) Ultrastructural localization of fibronectin and laminin in the basement membrane of the murine kidney. J Cell Biol 86:682–687
McAuslan BR, Gole GA (1980) Cellular and molecular mechanisms in angiogenesis. Trans Ophthalmol Soc UK 100:354–358
Melnick M, Jaskoll T, MacDougall M, Brownell A, Slavkin HC (1980) Fibronectin expression and distribution during chick limb development in vivo. Fed Proc 39: ABS # 3612
Moriarty G, Moriarty C, Sternberger L (1973) Ultrastructural immunocytochemistry with unlabeled antibodies and the peroxidase-antiperoxidase complex. J Histochem Cytochem 21:825–833
Ouchterlony O (1949) Antigen-antibody reactions in gels. Acta Pathol Microbiol Scand 26:507–511
Powell JT, Whitney PL (1980) Postnatal development of rat lung. Biochem J 188:1–8
Sainte-Marie G (1962) A paraffin embedding technique for studies employing immunofluorescence. J Histochem Cytochem 10:250–256
Sugiyama K, Kamata O, Katsuda N (1981) Fibronectin on the surface of rat mast cells. Exp Cell Res 133:449–452
Terranova VP, Rohrbach DH, Martin GR (1980) Role of laminin in the attachment of PAM 212 (epithelial) cells to basement membrane collagen. Cell 22:719–726
Thesleff I, Stenman S, Vaheri A, Timpl R (1979) Changes in the matrix proteins, fibronectin and collagen, during differentiation of mouse tooth germ. Dev Biol 70:116–126
Vaccaro C, Brody JS (1978) Ultrastructure of the developing alveoli. I. The role of the interstitial fibroblast. Anat Rec 192:467–480
Villiger B, Kelley DG, Engleman W, Kuhn C, McDonald JA (1981) Human alveolar macrophage fibronectin: Synthesis, secretion and ultrastructural localization during gelatin-coated latex particle binding. J Cell Biol 90:711–720
Wartiovarra J, Leivo I, Vaheri A (1979) Expression of the cell surface-associated glycoprotein fibronectin in early mouse embryos. Dev Biol 69:247–257
Weiss RE, Reddi AH (1980) Synthesis and localization of fibronectin during collagenous matrix-mesenchymal cell interaction and differentiation of cartilage and bone in vivo. Proc Natl Acad Sci US 77:2074–2078
Weiss RE, Reddi AH (1981) Role of fibronectin in collageneous matrix-induced mesenchymal cell proliferation and differentiation in vivo. Exp Cell Res 133:285–295
Williams MC, Mason RJ (1977) Development of the type II cell in the fetal rat lung. Am Rev Respir Dis 115:37–47
Yamada KM (1982) Fibronectin and other structural proteins in: Hay ED (ed) Cell biology of extracellular matrix. Plenum Press, New York, pp 95–110
Yamada KM, Olden K (1978) Fibronectins: Adhesive glycoproteins of the cell surface and blood. Nature 275:179–184
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rosenkrans, W.A., Albright, J.T., Hausman, R.E. et al. Light-microscopic immunocytochemical localization of fibronectin in the developing rat lung. Cell Tissue Res. 233, 113–123 (1983). https://doi.org/10.1007/BF00222236
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00222236