Cell and Tissue Research

, Volume 231, Issue 2, pp 451–456 | Cite as

Changes in the organization and ultrastructure of smooth muscle cells in the stomach of the gastric brooding frog, Rheobatrachus silus, during brooding

  • I. L. Gibbins
  • M. J. Tyler
Short Communication


The young of the aquatic Australian frog, Rheobatrachus silus (Leptodactylidae) develop from eggs to juvenile frogs in the mother's stomach. During brooding the stomach expands greatly and becomes very thin walled. Transmission electron microscopy showed that the distension of the stomach was accompanied by a severe disruption of the smooth muscle layers. Many of the smooth muscle cells seemed to be highly contracted and resembled smooth muscle cells contracted in the absence of an intact connective tissue matrix. Eight days after the birth of the juveniles through the mouth of the female, the stomach muscle cells had returned to a normal appearance. It is suggested that during gastric incubation of the young, smooth muscle cells become at least partially dissociated from their surrounding connective tissue matrix, allowing maximal distension of the stomach wall.

Key words

Smooth muscle Anura Stomach Electron microscopy 


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  1. Campbell G (1969) The autonomic innervation of the stomach of a toad (Bufo marinus). Comp Biochem Physiol 31:693–706Google Scholar
  2. Corben CJ, Ingram GJ, Tyler MJ (1974) Gastric brooding: a unique form of parental care in an Australian frog. Science 186:946–947Google Scholar
  3. Dixon WE (1902) The innervation of the frog's stomach. J Physiol 28:57–75Google Scholar
  4. Fanning JL, Tyler MJ, Shearman DJC (1982) Converting a stomach to a uterus: the microscopic structure of the stomach of the gastric brooding frog, Rheobatrachus silus. Gastroenterol 82:62–70Google Scholar
  5. Fay FS, Cooke PH, Canaday PG (1976) Contractile properties of isolated smooth muscle cells. In: Bülbring E, Shuba MF (eds) Physiology of smooth muscle. Raven Press, New York, pp 249–264Google Scholar
  6. Gabella G (1981) Structure of smooth muscles. In: Bülbring E, Brading AF, Jones AW, Tomita T (eds) Smooth muscle: an assessment of current knowledge. University of Texas Press, Austin, pp 1–46Google Scholar
  7. Gabella G, Raeymaekers L (1976) Effects of collagenase on mechanical activity and fine structure of an intestinal smooth muscle. Cell Tissue Res 173:29–44Google Scholar
  8. Gibbins IL (1980) The ultrastructural characterisation of peripheral non-adrenergic nerves; and the innervation of the amphibian small intestine and its development. PhD Thesis, University of MelbourneGoogle Scholar
  9. Goltz F (1872) Studien über die Bewegungen der Speiseröhre und des Magens des Frosches. Pflüger's Archiv 6:616–642Google Scholar
  10. Janes RG (1934) Studies on the amphibian digestive system. 1. Histological changes in the alimentary tract of anuran larvae during development. J Exp Zool 67:73–91Google Scholar
  11. Morishima K, Fujitani J (1908) Zur Untersuchungsmethode der spontanen Bewegung des Froschmagens. Arch Exp Pathol Pharm Suppl 407–414Google Scholar
  12. Patterson TL (1916) Contributions to the physiology of the stomach. XXXVI. The physiology of the gastric hunger contractions in the amphibia and reptilia. Comparative studies I. Am J Physiol 42:56–88Google Scholar
  13. Tyler MJ (1979) The impact of European man upon Australian amphibians. In: Tyler MJ (ed) The status of endangered Australasian wildlife. Roy Zool Soc South Australia, AdelaideGoogle Scholar
  14. Tyler MJ, Carter DB (1981) Oral birth of the young of the gastric brooding frog, Rheobatrachus silus. Anim Behav 29:280–282Google Scholar
  15. Uehara Y, Campbell GR, Burnstock G (1976) Muscle and its innervation: an atlas of fine structure. Edward Arnold, LondonGoogle Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • I. L. Gibbins
    • 1
  • M. J. Tyler
    • 2
  1. 1.Department of ZoologyUniversity of MelbourneAustralia
  2. 2.Department of ZoologyUniversity of AdelaideAustralia

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