Theoretical and Applied Genetics

, Volume 86, Issue 2–3, pp 141–149 | Cite as

Radiation-induced nonhomoeologous wheat-Agropyron intermedium chromosomal translocations conferring resistance to leaf rust

  • B. Friebe
  • J. Jiang
  • B. S. Gill
  • P. L. Dyck


The Agropyron intermedium chromosome 7Ai #2 is the source of the leaf rust resistance gene Lr38 which was transferred to wheat by irradiation. The chromosomal constitutions of eight radiation-induced rust-resistant wheat-Agropyron intermedium derivatives were analyzed by C-banding and genomic in-situ hybridization (GISH). Five lines were identified as wheat Ag. intermedium chromosome translocation lines with the translocation chromosomes T2AS·2AL-7Ai#2L, T5AL · 5AS-7Ai # 2L, T1DS · 1DL-7Ai # 2L, T3DL · 3DS-7Ai#2L, and T6DS · 6DL-7Ai#2L. The sizes of the 7Ai#2L segments in mitotic metaphases of these translocations are 2.42 μm, 4.20 μm, 2.55 μm, 2.78 μm, and 4.19 μm, respectively. One line was identified as a wheat-Ag. intermedium chromosome addition line. The added Ag. intermedium chromosome in this line is different from 7Ai # 2. This line has resistance to leaf rust and stem rust. Based on the rust reactions, and the C-banding and GISH results, the remaining two lines do not contain any Ag. intermedium-derived chromatin.

Key words

Common wheat Allen translocations Agropyron intermedium Leaf rust resistance In-situ hybridization C-banding 


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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • B. Friebe
    • 1
  • J. Jiang
    • 1
  • B. S. Gill
    • 1
  • P. L. Dyck
    • 2
  1. 1.Department of Plant Pathology, Throckmorton HallKansas State UniversityManhattanUSA
  2. 2.Agriculture CanadaWinnipegCanada

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