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Isolation and characterization of RAPD-based markers linked to the beet cyst nematode resistance locus (Hs1 pat-1) on chromosome 1 of B. patellaris

Theoretical and Applied Genetics volume 90, pages 885–891 (1995)Cite this article

Abstract

A beet cyst nematode (BCN)-resistant telosomic addition of B. patellaris chromosome 1 in B. vulgaris was used to isolate 6 RAPD markers linked to the BCN resistance locus Hs1 pat-1. Southern analysis showed that the analyzed RAPD products contain either low-, middle or high-repetitive DNA. The relative positions of the random amplified polymorphic DNA (RAPD) markers and of the restriction fragment length polymorphism (RFLP) loci corresponding to the low-repetitive RAPD products were determined by deletion mapping using a panel of seven nematode-resistant B. patellaris chromosome-1 fragment additions. One RAPD marker, OPB11800, was found to be present in two copies on the long arm telosome of B. patellaris chromosome 1. These copies are closely linked to the BCN resistance gene and flank the gene on both sides. On the basis of the nucleotide sequence of OPB11800, sequence-tagged site (STS) primers were developed that amplify specific fragments derived from the two OPB11800 loci. These STS markers can be used in the map-based cloning of the BCN gene, as they define start and finishing points of a chromosomal walk towards the Hs1 pat-1 locus. Two copies of the middle-repetitive OPX21100 marker were mapped in the same interval of the deletion mapping panel as the resistance gene locus and thereby belong to the nearest markers as yet found for the BCN gene in B. patellaris.

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References

  • Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218

    CAS  Google Scholar 

  • Baird E, Cooper-Bland S, Waugh R, DeMaine M, Powel W (1992) Molecular characterization of inter and ultra-specific somatic hybrids of potato using randomly amplified polymorphic DNA (RAPD) markers. Mol Gen Genet 233:469–475

    CAS  PubMed  Google Scholar 

  • Barone A, Ritter E, Schachtschabel U, Debner T, Salamini F, Gebhardt C (1990) Localization by restriction fragment length polymorphism mapping in potato of a major dominant gene conferring resistance to the potato cyst nematode Globodera rostochiensis. Mol Gen Genet 224:177–182

    Google Scholar 

  • Cooke DA (1991) The effect of beet cyst nematode, Heterodera Schachtii, on the yield of sugar beet in organic soils. Ann Appl Biol 118:153–160

    Google Scholar 

  • Coons GH (1975) Interspecific hybrids between Beta vulgaris L. and the wild species of Beta. J Am Soc Sugar Beet Technol 18:281–306

    Google Scholar 

  • De Jong JH, Speckmann GJ, De Bock ThSM, Lange W, Van Voorst A (1986) Alien chromosome fragments conditioning resistance to beet cyst nematode in diploid descendants from monosomic additions of Beta procumbens to Beta vulgaris. Can J Genet Cytol 28:439–443

    Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA mini-preparation: version II. Plant Mol Biol Rep 4:19–21

    Google Scholar 

  • Devos KM, Gale MD (1992) The use of random amplified polymorphic DNA markers in wheat. Theor Appl Genet 84:567–572

    Google Scholar 

  • Heijbroek W, Roelands AJ, De Jong JH, Van Hulst CG, Schoone AHL, Munning RG (1988) Sugar beets homozygous for resistance to beet cyst nematode (Heterodera schachtii Schm.), developed from monosomic additions of B. procumbens to B. vulgaris. Euphytica 38:121–131

    Google Scholar 

  • Hijner JA (1952) De gevoeligheid van wilde bieten voor het bietencysteaaltje (Heterodera schachtii). Meded Inst Ration Suikerprod 21:1–10

    Google Scholar 

  • Jung C, Koch R, Fisher F, Brandes A, Wricke G, Herrmann RG (1992) DNA markers closely linked to nematode resistance genes in sugar beet (Beta vulgaris L.) mapped using chromosome additions and translocations originating from beets of the Procumbentes section. Mol Gen Genet 232:271–278

    Google Scholar 

  • Klein-Lankhorst RM, Rietveld P, Verkerk R, Weide R, Gebhardt C, Koornneef M, Zabel P (1991a) RFLP markers linked to the root knot nematode resistance gene Mi in tomato. Theor Appl Genet 81:661–667

    Google Scholar 

  • Klein-Lankhorst RM, Vermunt A, Weide R, Liharska T, Zabel P (1991b) Isolation of molecular markers for tomato (L. esculentum) using random amplified polymorphic DNA (RAPD). Theor Appl Genet 83:108–114

    Google Scholar 

  • Klein-Lankhorst RM, Salentijn EMJ, Dirkse WG, Arens-de Reuver M, Stiekema WJ (1994) Construction of a YAC library from a Beta vulgaris fragment addition and isolation of a major satellite DNA cluster linked to the beet cyst nematode resistance locus Hs1 pat-1. Theor Appl Genet 89:426–434

    Google Scholar 

  • Kreike CM, De Koning JRA, Vinke JH, Van Ooijen JW, Gebhardt C, Stiekema WJ (1993) Mapping of loci involved in quantitatively inherited resistance to the potato cyst-nematode Globodera rostochiensis pathotype Rol. Theor Appl Genet 87:464–470

    Google Scholar 

  • Lange W, Jung C, Heijbroek W (1990) Transfer of beet cyst nematode resistance from Beta species of the section Patellares to cultivated beet. In:Proc 53rd Winter Congr. International Institute for Sugar Beet Research, pp 89–102

  • Lange W, Müller J, De Bock ThSM (1993) Virulence in the beet cyst nematode (Heterodera schachtii) versus some alien genes for resistance in beet. Fundam Appl Nematol 16:447–454

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Martin GB, Williams JGK, Tanksley SD (1991) Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines. Proc Natl Acad Sci USA 88:2336–2340

    Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic region by using segregating populations. Proc Natl Sci USA 88:9828–9832

    Google Scholar 

  • Olson M, Hood L, Cantor C, Botstein D (1989) A common language for physical mapping of the human genome. Science 245:1434–1435

    Google Scholar 

  • Overhauser J, Mewar R, Rojas K, Lia K, Kline AD, Silverman GA (1993) STS map of genes and anonymous DNA fragments on human chromosome 18 using a panel of somatic cell hybrids. Genomics 15:387–391

    Google Scholar 

  • Paran I, Michelmore RW (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85:985–993

    CAS  Google Scholar 

  • Paran I, Kesseli R, Michelmore RW (1991) Identification of restriction length polymorphism and random amplified polymorphic DNA markers linked to downy mildew resistance genes in lettuce, using near-isogenic lines. Genome 34:1021–1027

    Google Scholar 

  • Salentijn EMJ, Sandal NN, Lange W, De Bock ThSM, Krens FA, Marcker KA, Stiekema WJ (1992) Isolation of DNA markers linked to a beet cyst nematode resistance locus in Beta patellaris and Beta procumbens. Mol Gen Genet 235:432–440

    Google Scholar 

  • Salentijn EMJ, Sandal NN, Klein-Lankhorst RM, Lange W, De Bock ThSM, Marcker KA, Stiekema WJ (1994) Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on chromosome-1 of B. patellaris and B. procumbens. Theor Appl Genet 89:459–466

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    CAS  PubMed  Google Scholar 

  • Sarfatti M, Katan J, Fluhr R, Zamir D (1989) An RFLP marker in tomato linked to the Fusarium oxysporum resistance gene I2. Theor Appl Genet 78:755–759

    Google Scholar 

  • Schüller C, Backes G, Fischbeck G, Jahoor A (1992) RFLP markers to identify the alleles of the M1a locus conferring powdery mildew resistance in barley. Theor Appl Genet 84:330–338

    Google Scholar 

  • Speckmann GJ, De Bock ThSM, De Jong JH (1985) Monosomic additions with resistance to beet cyst nematode obtained from hybrids of Beta vulgaris and wild Beta species of the section Patellares. Z Pflanzenzuecht 95:74–83

    Google Scholar 

  • Toxopeus H, Lubberts H (1979) Breeding for resistance to the sugar beet nematode (Heterodera schachtii Schm.) in cruciferous crops. In: Eucarpia Cruciferae 1979 Conference. PUDOC, Wageningen, The Netherlands

    Google Scholar 

  • Vollrath D, Foote S, Hiltin A, Brown LG, Beer-Romero P, Bogan JS, Page DC (1992) The human Y chromosome: a 43-interval map based on naturally occurring deletions. Science 258:52–59

    Google Scholar 

  • Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218

    CAS  PubMed  Google Scholar 

  • Werner JE, Endo TR, Gill BS (1992) Towards a cytogenetically based physical map of the wheat genome. Proc Natl Acad Sci USA 89:11307–11311

    Google Scholar 

  • Wicking C, Williamson B (1991) From linked marker to gene. Trends Genet 7:288–293

    Google Scholar 

  • Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:7213–7218

    CAS  PubMed  Google Scholar 

  • Young ND, Zamir D, Ganal MW, Tanksley SD (1988) Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the Tm-2a gene in tomato. Genetics 120:579–587

    Google Scholar 

  • Yu MH (1984) Resistance to Heterodera schachtii in Patellares section of the genus Beta. Euphytica 33:633–640

    Google Scholar 

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Affiliations

  1. Department of Molecular Biology, DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, NL-6700 AA, Wageningen, The Netherlands

    E. M. J. Salentijn, M. J. B. Arens-De Reuver, W. J. Stiekema & R. M. Klein-Lankhorst

  2. Department of Arable and Fodder Crops, DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), PO Box 16, NL-6700 AA, Wageningen, The Netherlands

    W. Lange & Th. S. M. De Bock

Authors
  1. E. M. J. Salentijn
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  2. M. J. B. Arens-De Reuver
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  3. W. Lange
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  4. Th. S. M. De Bock
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  5. W. J. Stiekema
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  6. R. M. Klein-Lankhorst
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Communicated by F. Salamini

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Salentijn, E.M.J., Arens-De Reuver, M.J.B., Lange, W. et al. Isolation and characterization of RAPD-based markers linked to the beet cyst nematode resistance locus (Hs1 pat-1) on chromosome 1 of B. patellaris . Theoret. Appl. Genetics 90, 885–891 (1995). https://doi.org/10.1007/BF00222027

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  • DOI: https://doi.org/10.1007/BF00222027

Key words

  • Randomly amplified polymorphic DNA (RAPD)
  • Deletion mapping
  • Sequence Tagged Site (STS)
  • Monosomic fragment additions
  • Beet cyst nematode resistance
  • Heterodera schachtii Schm
  • Beta patellaris