Abstract
The inheritance of seed α-amylase inhibitor in the common bean and the genetic relationships among the variants and six arcelin variants in the common bean were investigated by crossing between accessions containing different αAI and arcelin variants. All seed proteins in parental, F1 and F2 seeds from the crosses were examined by Western-blot analysis. All F1 seeds gave combined αAI banding patterns from parents on the blotting membranes. The segregation of F2 seeds for αAI variants indicated that the polypeptides of αAI variants were inherited as single co-dominant units. Moreover, αAI and arcelin behaved as a single block in crosses, indicating a close linkage relationship between the genes controlling these proteins.
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References
Andreas JR, Yandell BS, Bliss FA (1986) Bean arcelin 1. Inheritance of a novel seed protein of Phaseolus vulgaris L. and its effect on seed composition. Theor Appl Genet 72:123–128
Cardona C, Kornegay J, Posso CE, Morales F, Ramirez H (1990) Comparative value of four arcelin variants in the development of dry bean lines resistant to the Mexican bean weevil. Entomol Exp Appl 56:197–206
Chrispeels MJ, Raikhel NV (1991) Lectins, lectin genes, and their role in plant defense. Plant Cell 3:1–9
Hartweck LM, Vogelzang RD, Osborn TC (1991) Characterization and comparison of arcelin seed protein variants from common bean. Plant Physiol 97:204–211
Hoffman LM, Donaldson DD (1985) Characterization of two Phaseolus vulgaris phytohemagglutinin genes closely linked on the chromosome. EMBO J 4:883–889
Hoffman LM, Ma Y, Barker RF (1982) Molecular cloning of Phaseolus vulgaris lectin mRNA and use of cDNA as a probe to estimate lectin transcript levels in various tissues. Nucleic Acids Res 10:7819–7828
Ishimoto M, Kitamura K (1988) Identification of the growth inhibitor on azuki bean weevil in kidney bean (Phaseolus vulgaris L.). Japan J Breed 38:367–370
Ishimoto M, Kitamura K (1989) Growth inhibitory effects of an α-amylase inhibitor from kidney ban, Phaseolus vulgaris (L.) on the three species of bruchids (Coleoptera: Bruchidae). Appl Entomol Zool 24:281–286
Ishimoto M, Kitamura K (1991) Effects of absence of seed α-amylase inhibitor on the growth inhibitory activity to azuki bean weevil (Callosobruchus chinensis) in common bean (Phaseolus vulgaris L.). Japan J Breed 41:231–240
Ishimoto M, Kitamura K (1993) Specific inhibitory activity and inheritance of an α-amylase inhibitor in a wild common bean accession resistant to the Mexican bean weevil. Japan J Breed 43:69–73
Ishimoto M, Suzuki K, Iwanaga M, Kikuchi F, Kitamura K (1995) Variation of seed α-amylase inhibitor in common bean. Theor Appl Genet 90:425–429
Kijimoto-Ochiai S, Katagiri YU, Ochiai H (1985) Analysis of N-linked oligosaccharide chains of glycoprotein on nitrocellulose sheets using lectin-peroxidase reagents. Anal Biochem 143:222–229
Kornegay J, Cardona C, Posso CE (1993) Inheritance of resistance to the Mexican bean weevil in common bean, determined by bioassay and biochemical tests. Crop Sci 33:589–594
Mather K (1951) The measurement of linkage in heredity. Wiley and Sons, New York.
Minney BHP, Gatehouse AMR, Dobie P, Dendy J, Cardona C, Gatehouse JA (1990) Biochemcial basis of seed resistance to Zabrotes subfasciatus (been weevil) in Phaseolus vulgaris (common bean), a mechanism for arcelin toxicity. J Insect Physiol 36:757–767
Nodari RO, Tsai SM, Gilbertson RL, Gepts P (1993) Towards an integrated linkage map of common beam 2. Development of an RFLP-based linkage map. Theor Appl Genet 85:513–520
Osborn TC, Blake T, Gepts P, Bliss FA (1986) Bean arcelin. 2. Genetic variation, inheritance and linkage relationships of a noval seed protein of Phaseolus vulgaris L. Theor Appl Genet 71:847–855
Osborn TC, Alexander DC, Sun SSM, Cardona C, Bliss FA (1988) Insecticidal activity and lectin homology of arcelin seed protein. Science 240:207–210
Russell DR, Wallace KM, Bathe JH, Martinell BJ, McCabe DE (1993) Stable transformation of Phaseolus vulgaris via electric-discharge-mediated particle acceleration. Plant Cell Rep 12:165–169
Schroeder HE, Schotz AH, Wardley-Richardson T, Spencer D, Higgins TJV (1993) Transformation and regeneration of two cultivars of pea (Pisum sativum L.) Plant Physiol 101:751–757
Suzuki K, Ishimoto M, Kikuchi F, Kitamura K (1993) Growth inhibitory effect of an α-amylase inhibitor from the wild common bean resistant to the Mexican bean weevil (Zabrotes subfasciatus). Japan J Breed 43:257–265
Suzuki K, Ishimoto M, Kitamura K (1994) cDNA sequence and deduced primary structure of an α-amylase inhibitor from a bruchid-resistant wild common bean. Biochim Biophys Acta 1206:289–291
Toro O, Tohme J, Debouck DG (1990) Wild bean (Phaseolus vulgaris L.): description and distribution. CIAT Publication, No. 181 CIAT, Colombia
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Communicated by G. A. Kush
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Suzuki, K., Ishimoto, M., Iwanaga, M. et al. Inheritance of seed α-amylase inhibitor in the common bean and genetic relationship to arcelin. Theoret. Appl. Genetics 90, 762–766 (1995). https://doi.org/10.1007/BF00222009
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DOI: https://doi.org/10.1007/BF00222009