Abstract
A species-specific repeated sequence, pHvNAU62, was cloned from Haynaldia villosa, a wheat relative of great importance. It strongly hybridized to H. villosa, but not to wheat. In situ hybridization localized this sequence to six of seven H. villosa chromosome pairs in telomeric or sub-telomeric regions. Southern hybridization to whea-H. villosa addition lines showed that chromosomes 1V through 6V gave strong signals in ladders while chromosome 7V escaped detection. In addition to H. villosa, several Triticeae species were identified for a high abundance of the pHvNAU62 repeated sequence, among which Thinopyrum bassarabicum and Leymus racemosus produced the strongest signals. Sequence analysis indicated that the cloned fragment was 292 bp long, being AT rich (61%), and showed 67% homology of pSc7235, a rye repeated sequence. Isochizomer analysis suggested that the present repeated sequence was heavily methylated at the cytosine of the CpG dimer in the genome of H. villosa.It was also demonstrated that pHvNAU62 is useful in tagging the introduced 6VS chromosome arm, which confers a resistance gene to wheat powdery mildew, in the segregating generations.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anamthawat-Jonsson K, Heslop-Harrison JS (1993) Isolation and characterization of genome-specific DNA sequences in Triticeae species. Mol Gen Genet 240:151–158
Apples R, Dennis ES, Smith DR, Peacock WJ (1981) Two repeated DNA sequences from the heterochromatin region of rye (Secale cereale) chromosomes. Chromosoma 84:256–277
Appels R, Moran LB, Gustafson PJ (1986) Rye heterochromatin. I. Studies on clusters of the major repeating sequence and the identification of a new dispersed repetitive sequence element. Can J Genet Cytol 28:645–657
Bedbrook JR, O'Dell M, Thompson R, Flavell RB (1980) A molecular description of telomeric heterochromatin in Secale cereale. Cell 19:545–560
Blanco A, Simeone R, Resta P (1987) The addition of Dasypyrum villosum (L.) Candargy chromosomes to durum wheat (Triticum durum Desf.). Theor Appl Genet 74:328–333
Blanco A, Resta P, Simeone R, Parmar S, Shewry PR, Sabelli P, Lafiandra D (1991) Chromsomal location of seed storage protein genes in the genome of Dasypyrum villosa (L) Candargy. Theor Appl Genet 82:358–362
Chen PD, Liu DJ (1986) Identification of Haynaldia villosa chromosomes in wheat alien addition lines. In: Li Zhensheng (ed) Proc 1st Int Symp on Chromosome Engineering in Plants, Xian, China, pp 31–33
De Pace C, Benedettelli S, Qualset CO, Hart GE, Scarascia Mugnozza GT, Delre V, Vittori D (1988) Biochemical markers in Triticum x Dasypyrum amphiploid and derived disomic addition lines. In: Miller TE, Koebner RMD (eds) Proc 7th Int Wheat Genet Symp, Cambridge, UK, pp 503–509
De Pace C, Deler V, Scarascia Mugnozza GT, Qualset CO, Cermonini R, Frediani M, Cionini PG (1992) Molecular and chromosomal characterization of repeated and single-copy DNA sequences in the genome of Dasypyrum villosum. Hereditas 116:55–65
Flavell RB (1986) Repeated sequences and chromosome evolution in plants. Trans R Soc Lond B 312:227–242
Friebe B, Cermeno MC, Zeller FJ (1987) C-banding polymorphism and the analysis of nucleolar activation in Dasypyrum villosum (L.) Candargy, its added chromosomes to hexaploid wheat and the amphiploid Triticum dicoccum-D. villosum. Theor Appl Genet 73:337–342
Gerlach WL, Peacock WJ (1980) Chromosomal locations of highly repeated DNA sequences in wheat. Heredity 44:269–276
Gill KS, Lubbers EL, Gill BS, Raupp WJ, Cox TS (1991) A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD). Genome 34:362–374
Guidet F, Rogowsky P, Taylor C, Song W, Langridge P (1991) Cloning and characterization of a new rye-specific repeated sequence. Genome 34:81–87
Hyde BB (1953) Addition of individual Haynaldia villosa chromosomes to hexaploid wheat. Am J Bot 40:174–182
Jones JDG, Flavell RB (1982) The mapping of highly-repeated DNA families and their relationship to C-bands in chromosomes of Secale cereale. Chromosoma 86:592–612
Lapitan NLV (1992) Organization and evolution of higher plant nuclear genomes. Genome 35:171–181
Liu DJ, Chen PD, Wang YN, Qiu BX, Wang SL (1988) Transfer of Haynaldia villosa chromosomes into Triticum aestivum. In: Miller TE, Koebner RMD (eds) Proc 7th Int Wheat Genet Symp, Cambridge, UK, pp 355–361
Liu DJ, Chen PD, Raupp J (1993) Determination of homologous groups of Haynaldia villosa chromosomes. In: Xin ZY (ed) Proc 8th Int Wheat Genet Symp, Beijing, China (in press)
McIntyre CL, Pereira S, Moran LN, Appels R (1990) New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat. Genome 33:635–640
McNeil D, Lagudah ES, Hohmann U, Appels R (1994) Amplification of DNA sequences in wheat and its relatives: the Dgas44 and R350 families of repetitive sequences. Genome 37:320–327
Montebrove L, De Pace C, Jan CC, Scarascia Mugnozza GT, Qualset CO (1987) Chromosomal location of isozymes and seed storage protein genes in Dasypyrum villosum (L.) Candargy. Theor Appl Genet 73:836–845
Mukai Y, Endo TR, Gill BS (1991) Physical mapping of the 18s, 26s rRNA multigene family in common wheat: identification of a locus. Chromosoma 100:71–78
Pardue ML, Gall JG (1970) Chromosomal localisation of mouse satellite DNA. Science 168:1356–1358
Qi LL, Chen PD, Liu DJ, Zhou B, Zhang SZ (1993) Development of translocation lines of Triticum aestivum with powdery mildew resistance introduced from Haynaldia villosa. In Xin ZY (ed) Proc 8th Int Wheat Genet Symp, Beijing, China (in press)
Qualset CD, De Pace CC, Jan CC (1981) Haynaldia villosa (L.) Schur, A species with potential use in wheat breeding. Am Soc Agr Abstr, 70
Rayburn AL, Gill BS (1986) Molecular identification of the Dgeneome chromosomes of wheat. J Hered 77:253–255
Sambrook J, Fristsch EF, Maniates T (1989) Molecular cloning: a laboratory manual. 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sharp PJ, Kreis M, Sehwry PR, Gale MD (1988) Location of betaamylase sequences in wheat and its relatives. Theor Appl Genet 75:286–290
Talbert LE, Clack TH (1991) Identification of D-genome chromosomes on Chinese Spring of Southern blots using genome-specific repetitive DNA. J Hered 82:509–512
Tsujimoto H, Gill BS (1991) Repetitive DNA sequences from polyploid Elymus trachycaulus and the diploid progenitor speices: detection and genomic affinity of Elymus chromatin added to wheat. Genome 34:782–789
Vilageliu L, Tyler-Smith C (1992) Structural instability of YAC clones and use of recombination-deficient yeast host strains. In: Rakesh Anand (ed) Techniques for the analysis of complex genomes. Academic Press, San Diego, pp 93–112
Zhang HB, Dvorak J (1990) Isolation of repeated DNA sequences from Lophopyrum elongatum for detection of Lophopyrum chromatin in wheat. Genome 33:283–293
Author information
Authors and Affiliations
Additional information
Communicated by K. Tsunewaki
Rights and permissions
About this article
Cite this article
Li, W.L., Chen, P.D., Qi, L.L. et al. Isolation, characterization and application of a species-specific repeated sequence from Haynaldia villosa . Theoret. Appl. Genetics 90, 526–533 (1995). https://doi.org/10.1007/BF00221999
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00221999