Abstract
DNA restriction endonuclease fragment analysis was used to obtain new information on the genomic organization of Vicia ribosomal DNA (rDNA), more particularly among V. faba and its close relatives and the taxa within three (Narbonensis, Villosa, Sativa) species' complexes. Total genomic DNA of 90 accessions representing 49 Vicia species was restricted with 11 enzymes, and the restriction fragments were probed with three ribosomal clones. Twenty-eight repeat unit length classes were identified. The number of length classes (1–2) per accession did not correspond to the number of nucleolar organizing regions (NORs). The number of rRNA genes was independent of the 2C nuclear DNA amount present in the taxon. Each of the 90 accessions had 2 (rarely 1)-4 DraI sites. Those taxa with the same number of DraI sites generally could be distinguished from each other by different configurations. Probing of the DNA samples digested with tetranucleotide recognition restriction endonucleases emphasized differences between divergent spacer regions and enabled relative homologies between the coding regions to be established. Overall, rDNA restriction site variation among the species showed a good correlation with taxonomic classification. The rDNA analysis indicated evolutionary relatedness of the various taxa within the Narbonensis species complex. rDNA diversity within two other species complexes (Villosa, Sativa), on the other hand, was more extensive than expected. With few exceptions, data on the two complexes give evidence of taxon-specific divergences not seen with other approaches. The restriction site variability and repeat length heterogeneity in the rDNA repeat exhibited startling differences between V.faba and its close wild relatives included in the Narbonensis species complex. This analysis provides new evidence that none of the species within the complex can be considered to be putative allies of broad bean.
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References
Allkin R, Goyder DJ, Bisby FA, White RJ (1986) Names and synonyms of species and subspecies in the Vicieae: Issue 3, Vicieae Database Project, Publication No. 7
Appels R, Dvorák J (1982a) The wheat ribosomal DNA spacer region: Its structure and variation in populations and among species. Theor Appl Genet 63:337–348
Appels R, Dvorák J (1982b) Relative rates of divergence of spacer and gene sequences within the rDNA region of species in the Triticeae: implications for the maintenance of homogeneity of a repeated gene family. Theor Appl Genet63:361–371
Appels R, Honeycutt RL (1986) rDNA: Evolution over a billion years. In: Dutta SK (ed) DNA systematics, vol 2. CRC Press, Boca Raton, Fla., pp 81–135
Arnheim N, Krystal M, Schmickel R, Wilson G, Ryder O, Zimmer E (1980) Molecular evidence for genetic exchanges among ribosomal genes on non-homologous chromosomes in man and apes. Proc Natl Acad Sci USA 77:7323–7327
Baker HG (1970) Genetic resources in plants: their exploration and conservation. In: Frankel OH, Bennett E (eds) Taxonomy and the biological species concept in cultivated plants. Blackwell Scientific Publ, Oxford, pp 49–68
Birch ANE, Tithecott MT, Bisby FA (1985) Vicia johannis and wild relatives of Faba bean: a taxonomic study. Econ Bot 39:177–190
Breiman A, Rotem-Abbarbanell D, Karp A, Shaskin H (1987) Heritable somaclonal variation in wild barely (Hordeum spontaneum). Theor Appl Genet 74:104–112
Chooi WY (1971) Variatioin in nuclear DNA content in the genus Vicia. Genetics 68:195–211
Cubero JI (1981) Interspecific hybridization in Vicia. In: Hawton G, Webb C (eds) Faba bean improvement. Martinus Nijhoff, The Netherlands, pp 91–108
Cubero JI (1984) Taxonomy, distribution and evolution of the Faba bean and its wild relatives. In: Witcombe JR, Erskine W (eds) Genetic resources and their exploitation chick peas, faba beans and lentils. Martinus Nijhoff/Dr. W Junk Publ, The Netherlands, pp 131–143
Doyle JJ, Beachy RN (1985) Ribosomal gene variation in soybean (Glycine) and its relatives. Theor Appl Genet 70:369–376
Doyle JJ, Beachy RN, Lewis WH (1984) Evolution of rDNA in Claytonia polyploid complexes. In: Grant WF (ed) Plant biosystematics. Academic Press, Ontario, Canada, pp 321–341
Duke JA (1981) Handbook of legumes of world economic importance. Plenum Press, New York
Ellis THN, Davies DR, Castleton JA, Bredford ID (1984) The organization and genetics of rDNA length variants in peas. Chromosoma 91:74–81
Gerlach WL, Bedrock JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7:1869–1885
Gill BS, Appels R (1988) Relationships between Nor-loci from different Triticeae species. Plant Syst Evol 160:77–89
Gruenbaun Y, Naveh-Many T, Cedar H, Razin A (1981) Sequence specificity of methylation in higher plant DNA. Nature 292:860–862
Hamby RK, Zimmer EA (1988) Ribosomal RNA sequences for inferring phylogeny within the grass family (Poaceae). Plant Syst Evol 160:29–37
Hanelt P, Mettin D (1966) Cytosystematische Untersuchungen in der Artengruppe um Vicia sativa L. II. Kulturpflanzen 14:137–161
Hanelt P, Mettin D (1989) Biosystematics of the genus Vicia L. (Leguminosae). Annu Rev Ecol Syst 20:199–223
Harlan JR (1956) Theory and dynamics of grassland agriculture. D Van Nostrand, Princeton
Hollings E, Stace CA (1974) Karyotype variation and evolution in the Vicia sativa aggregate. New Phytol 73:195–208
Johns MA, Strommer JN, Freeling M (1983) Exceptionally high levels of restriction site Polymorphism in DNA near the maize AdhI gene. Genetics 105:733–743
Kato A, Yakura K, Tanifuji S (1985) Repeated DNA sequences found in the large spacer of Vicia faba rDNA. Biochem Biophys Acta 825:411–415
Kim WK, Innes RL, Kerber ER (1992) Ribosomal DNA repeat unit polymorphism in six Aegilops species. Genome 35:510–515
Kupicha FK (1976) The infrageneric structure of Vicia. Notes R Bot Gard Edinburgh 34:287–326
Ladizinsky G (1975) Seed protein electrophoresis of the wild and cultivated species of section Faba of Vicia. Euphytica 24:785–788
Lapitan NLV, Gill BS, Sears RG (1987) Genomic and phylogenetic relationships among rye and perennial species in the Triticeae. Crop Sci 27:682–687
Long EO, Dawid IB (1980) Repeated genes in eukaryotes. Annu Rev Biochem 49:727–764
Maher EP, Fox DP (1973) Multiplicity of ribosomal RNA genes in Vicia species with different nuclear DNA contents. Nature 245:170–172
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Press, Cold Spring Harbor, N.Y.
Maxted N (1993) A phenetic investigation of Vicia L. subgenus Vicia (Leguminosae, Vicieae). Bot J Linn Soc 111:155–182
Maxted N, Khattab AMA, Bisby FA (1991) The newly discovered relatives of Vicia faba L. do little to resolve the enigma of its origin. Bot Chron 10:435–465
Molnar SJ, Fedak G (1989) Polymorphism in ribosomal DNA repeat units of 12 Hordeum species. Genome 32:1124–1127
Molnar SJ, Gupta PK, Fedak G, Wheatcroft R (1989) Ribosomal DNA repeat unit polymorphism in 25 Hordeum species. Theor Appl Genet 78:387–392
Murray HG, Thompson WF (1980) Rapid isolation of high-molecular-weight plant DNA. Nucleic Acids Res 8:4321–4325
Narayan RKJ, Ramachandran C, Raina SN (1985) The distribution of satellite DNA in the chromosome complements of Vicia species (Leguminosae). Genetica 66:115–121
Perrino P, Maruca G, Linsalata V, Bianco VV, Lester RN, Lattanzio V (1989) Flavonoid taxonomic analysis of Vicia species of section Faba. Can J Bot 67:3529–3533
Plitmnn U (1967) Biosystematical study in the annual species of Vicia of the Middle East. Hebrew University, Jerusalem
Raina SN (1990) Genome organisation and evolution in the genus Vicia. In: Kawano S (ed) Biological approaches and evolutionary trends in plants. Academic Press, London, pp 183–201
Raina SN, Bisht MS (1988) DNA amounts and chromatin compactness in Vicia. Genetica 77:65–77
Rains SN, Narayan RKJ (1984) Changes in DNA composition in the evolution of Vicia species. Theor Appl Genet 68:187–192
Raina SN, Ogihara Y (1994) Chloroplast DNA diversity in Vicia faba and its close wild relatives: implications for reassessment. Theor Appl Genet 88:261–266
Raina SN, Rees H (1983) DNA variation between and within chromosome complements of Vicia species. Heredity 51:335–346
Raina SN, Yamamoto K, Murakami M (1989) Intraspecific hybridization and its bearing on chormosome evolution in Vicia narbonensis (Fabaceae). Plant Syst Evol 167:201–217
Reed KC, Mann DA (1985) Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res 3:7207–7221
Rogers SO, Bendich AJ (1987a) Heritability and variability in ribosomal RNA genes of Vicia faba. Genetics 117:285–295
Rogers SO, Bendich AJ (1987b) Ribosomal RNA genes in plants: variability in copy number and in the intergenic spacer. Plant Mol Biol 9:509–520
Rogers SO, Honda S, Bendich AJ (1986) Variation in the ribosomal RNA genes among individuals of Vicia faba. Plant Mol Biol 6:339–345
Roti-Michelozzi G (1986) Biosystematic studies on the Vicia villosa complex in Europe. Candollea 41:399–411
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Santoni S, Bervillé A (1992) Characterization of the nuclear ribosomal DNA units and phylogeny of Beta L. wild forms and cultivated beets. Theor Appl Genet 83:533–542
Schaal BA, Learn GH Jr (1988) Ribosomal DNA variation within and among plant populations. Ann Mo Bot Gard 75:1207–1216
Schäfer HI (1973) Zür Taxonomie der Vicia narbonensis Gruppe. Kulturpflanzen21:211–273
Springer PS, Zimmer EA, Bennetzen JL (1989) Genomic organization of the ribosomal DNA of Sorghum and its close relatives. Theor Appl Genet 77:844–850
Sytsma KJ, Schaal BA (1985) Phylogenetics of the Lisianthius skinners (Gentianaceae) species complex in Panama utilizing DNA restriction fragment analysis. Evolution 39:594–608
Terauchi R, Chikaleke VA, Thottappilly G, Hahn SK (1992) Origin and phylogeny of Guinea yams as revealed by RFLP analysis of chloroplast DNA and nuclear ribosomal DNA. Theor Appl Genet 83:743–751
van de Ven WTG, Duncan N, Ramsay G, Phillips M, Powell W, Waugh R (1993) Taxonomic relationships between V.faba and its relatives based on nuclear and mitochondrial RFLPs and PCR analysis. Theor Appl Genet 86:71–80
van de Ven WTG, Powell W, Ramsay G, Waugh R (1990) Restriction fragment length polymorphisms as genetic markers in Vicia. Heredity 65:329–342
Yakura K, Tanifuji S (1981) The organization of rRNA genes in Vicia faba. Plant Cell Physiol 22:1105–1111
Yakura K, Tanifujii S (1983) Molecular cloning and restriction analysis of EcoRI fragments of Vicia faba rDNA. Plant Cell Physiol 24:1327–1330
Yakura K, Kato A, Tanifuji S (1984) Length heterogeneity in the large spacer of Vicia faba rDNA is due to the differing number of 325-bp repetitive sequence element. Mol Gen Genet 193:400–405
Zimmer EA, Jupe ER, Walbot V (1988) Ribosomal gene structure, Variation and inheritance in maize and its ancestors. Genetics 120:1125–1136
Zohary D, Hopf M (1973) Domestication of pulses in the old world. Science 182:887–894
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Raina, S.N., Ogihara, Y. Ribosomal DNA repeat unit polymorphism in 49 Vicia species. Theoret. Appl. Genetics 90, 477–486 (1995). https://doi.org/10.1007/BF00221993
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DOI: https://doi.org/10.1007/BF00221993