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Localization of genes for bacterial canker resistance in Lycopersicon peruvianum using RFLPs

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Abstract

A backcross population of the L. peruvianum accession LA 2157, which is resistant to bacterial canker caused by Clavibacter michiganensis ssp. michiganensis, with the susceptible L. peruvianum accession LA 2172 was evaluated for the segregation of C. michiganenis resistance and of RFLP markers in order to map the loci involved in this resistance. The development of symptoms of the disease was scored using an ordinal scale. The mapping of the disease resistance was hampered by distorted segregation ratios of a large number of markers and unexpected quantitative inheritance of the resistance. By means of the Kruskal-Wallis rank-sum test, five regions on chromosomes 1, 6, 7, 8 and 10 were identified that may be involved in C. michiganensis resistance.

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References

  • Berry SZ, Madumadu GG, Uddin MR, Coplin DL (1989) Virulence studies and resistance to Clavibacter michiganensis ssp. michiganensis in tomato germplasm. Hortic Sci 24:362–365

    Google Scholar 

  • Bonierbale MW, Plaisted RL, Tanksley SD (1988) RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120:1095–1103

    Google Scholar 

  • Gebhardt C, Ritter E, Barone A, Debener T, Walkemeier B, Schachtschabel U, Kaufmann H, Thompson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini F (1991) RFLP maps of potato and their alignment with the homoeologous tomato genome. Theor Appl Genet 83:49–57

    Google Scholar 

  • Hogenboom NG (1973) A model for incongruity in intimate partner relationships. Euphytica 22:219–233

    Google Scholar 

  • Laterrot H, Brand R, Daunay MC (1978) La resistance a Corynebacterium michiganense chez la tomate. Etude bibliographique. Ann Amel Plant 28:579–591

    Google Scholar 

  • Lehmann EL (1975) Nonparametrics. McGraw-Hill, New York

    Google Scholar 

  • Lin SY, Ikehashi H, Yanagihara S, Kawashima A (1992) Segregation distortion via male gametes in hybrids between Indica and Japonica or wide-compatibility varieties of rice (Oryza saliva L.). Theor Appl Genet 84:812–818

    Google Scholar 

  • Lindhout P, Purimahua C (1989) Investigations on tomato breeding for resistance to Corynebacterium michiganense. In: Book of poster abstracts, part II, 12th EUCARPIA Cong Sci Plant Breed. Göttingen, Germany, p 16–8

  • Löffler HJM, van den Bulk RW, Kerckhoffs D, Purimahua C, Lindhout WH (1989) A fast screening method for the detection of resistance to Clavibacter michiganensis in tomato. In: Klement Z (ed) Proc 7th Int Conf Plant Pathol Budapest, Hungary, pp 957–962

  • Miller JC, Tanksley SD (1990) RFLP analysis of phylogentic relationships and genetic variation in the genus Lycopersicon. Theor Appl Genet 80:437–448

    CAS  Google Scholar 

  • Osborn TC, Alexander DC, Forbes JF (1987) Identification of restriction fragment length polymorhisms linked to genes controlling soluble solid content in tomato fruit. Theor Appl Genet 73:350–356

    Google Scholar 

  • Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726

    Article  CAS  PubMed  Google Scholar 

  • Paterson AH, DeVerna JW, Lanini B, Tanksley SD (1990) Fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes, in an interspecies cross of tomato. Genetics 124:735–742

    Google Scholar 

  • Paterson AH, Damon S, Hewitt JD, Zamir D, Rabinowitch HD, Lincoln SE, Lander ES, Tanksley SD (1991) Mendelian factors underlying quantitative traits in tomato: comparison across species, generations, and environments. Genetics 127:181–197

    Google Scholar 

  • Patton DA, Franzmann LH, Meinke DW (1991) Mapping genes essential for embryo development in Arabidopsis thaliana. Mol Gen Genet 227:337–347

    Google Scholar 

  • Rick CM (1986) Reproductive isolation in the Lycopersicon peruvianum complex. In: D'Arcy WG (ed) Solanaceae, biology and systematics. Columbia University Press, New York, pp 477–495

    Google Scholar 

  • Stamova L, Yordanov M (1985) A new source of resistance to Corynebacterium michiganense (E.F. Sm.). Plant Sci 22:70

    Google Scholar 

  • Tanksley SD, Loaiza-Figueroa F (1985) Gametophytic self-incompatibility is controlled by a single locus on chromosome 1 in Lycopersicon peruvianum. Proc Natl Acad Sci USA 82:5093–5096

    CAS  Google Scholar 

  • Tanksley SD, Mutschler MA (1990) Linkage map of the tomato (Lycopersicon esculentum) (2n = 24). In: O'Brien SJ (ed) Genetic maps; locus maps of complex genomes. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y., pp 6.3–6.15

    Google Scholar 

  • Tanksley SD, Ganal MW, Prince JP, Vicente MC de, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Röder MS, Wing RA, Wu W, Young ND (1992) High-density molecular linkage maps of the tomato and potato genomes. Genetics 132:1141–1160

    CAS  PubMed  Google Scholar 

  • Thyr BD (1968) Resistance to Corynebacterium michiganense measured in six Lycopersicon accessions. Phytopathology 61:972–974

    Google Scholar 

  • Thyr BD (1969) Additional sources of resistance to bacterial canker of tomato (Corynebacterium michiganense). Plant Dis Rep 53:234–237

    Google Scholar 

  • Van den Bulk RW, Löffler HJM, Dons JJM (1989) Effect of phytotoxic compounds produced by Clavibacter michiganensis ssp. michiganensis on resistant and susceptible tomato plants. Neth J Plant Pathol 95:107–117

    Google Scholar 

  • Van der Beek JG, Verkerk R, Zabel P, Lindhout P (1992) Mapping strategy for resistance genes in tomato based on RFLPs between cultivars: Cf9 (resistance to Cladosporium fulvum) on chromosome 1. Theor Appl Genet 84:106–112

    Google Scholar 

  • Van Ooijen JW, Sandbrink JM, Purimahua CC, Vrielink M, Verkerk R, Zabel P, Lindhout P (1994) An RFLP linkage map of Lycopersicon peruvianum. Theor Appl Genet 89:1007–1013

    Google Scholar 

  • Vulkova ZV, Sotirova VG (1993) Study of the three-genome hybrid Lycopersicon esculentum Mill. — L. chilense Dun. — L. peruvianum var ‘humifusum’ Mill, and its use as a source for resistance. Theor Appl Genet 87:337–342

    Google Scholar 

  • Young ND, Zamir D, Ganal MW, Tanksley SD (1988) Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the Tm-2a gene in tomato. Genetics 120:559–585

    Google Scholar 

  • Zamir D, Tadmor Y (1986) Unusual segregation of nuclear genes in plants. Bot Gaz 147:355–358

    Article  Google Scholar 

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Communicated by J. W. Sanpe

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Sandbrink, J.M., van Ooijen, J.W., Purimahua, C.C. et al. Localization of genes for bacterial canker resistance in Lycopersicon peruvianum using RFLPs. Theoret. Appl. Genetics 90, 444–450 (1995). https://doi.org/10.1007/BF00221988

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  • DOI: https://doi.org/10.1007/BF00221988

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