Abstract
Two diploid (2n=18) sugar beet (Beta vulgaris L.) lines which carry monogenic traits for nematode (Heterodera schachtii Schm.) resistance located on translocations from the wild beet species Beta procumbens were investigated. Short interspersed repetitive DNA elements exclusively hybridizing with wild beet DNA were found to be dispersed around the translocations. The banding pattern as revealed by genomic Southern hybridization was highly conserved among translocation lines of different origins indicating that the translocations are not affected by recombination events with sugar beet chromosomes. Physical mapping revealed that the entire translocation is represented by a single Sal I fragment 300 kb in size. A representative YAC (yeast artifical chromosome) library consisting of approximately 13,000 recombinant clones (2.2 genome equivalents) with insert sizes ranging between 50 and 450 kb and an average of 130kb has been constructed from the resistant line A906001. Three recombinant YACs were isolated from this library using the wild beet-specific repetitive elements as probes for screening. Colinearity between YAC inserts and donor DNA was confirmed by DNA fingerprinting utilizing these repetitive probes. The YACs were arranged into two contigs with a total size of 215 kb; these represent a minimum of 72% of the translocation.
Similar content being viewed by others
References
Arondel V, Lemieux B, Hwang I, Gibson S, Goodman HM, Somerville CR, (1992) Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis. Science 258:1353–1355
Arumuganathan K, Earle ED (1991) Nuclear DMA content of some important plant species. Plant Mol Biol Rep 9:208–218
Bellanné-Chantelot C, Lacroix B, Ougen P, Billaut A, Beaufils S, Bertrand S, Georges I, Glibert F, Gros I, Lucotte G, Susini L, Codani JJ, Gesnouin P, Pook S, Vaysseix G, Lu-Kuo J, Ried T, Ward D, Chumakov I, Le Paslier D, Barillo E, Cohen D (1992) Mapping the whole human genome by fingerprinting yeast artificial chromosomes. Cell 70:1059–1068
Benton WD, Davis RW (1977) Screening lambda gt recombinant clones by hybridization to single plaques in situ. Science 196:180
Brandes A, Jung C, Wricke G (1987) Nematode resistance derived from wild beet and its meiotic stability in sugar beet. Plant Breed 99:56–64
Burke DT, Carle GF, Oison MV (1987) Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236:806–812
Chumakov I, Rigault P, Guillou S, Ougen P, Billaut A, Guasconi G, Gervy P, LeGall I, Soularue P, Grinas L, Bougueleret L, Bellanné Chantelot C, Lacroix B, Barillot E, Gesnouin P, Pook S, Vaysseix G, Frelat G, Schmitz A, Sambucy J-L, Bosch A, Estivill X, Weissenbach J, Vignal A, Riethman H, Cox D, Patterson D, Gardiner K, Hattori M, Sakaki Y, Ichikawa H, Le Paslier D, Heilig R, Antonarakis S, Cohen D (1992) Continuum of overlapping clones spanning the entire human chromosome 21q. Nature 359:380–387
Cohen D, Chumakov I, Weissenbach J (1993) A first-generation physical map of the human genome. Nature 366:698–701
de Jong JH, Speckmann GJ, De Bock TSM, Lang W, Van Voorst A (1986) Alien chromosome fragments conditioning resistance to beet cyst nematode in diploid descendants from monosomic additions of Beta procumbens to B. vulgaris. Can J Genet Cytol 28:439–443
Edwards KJ, Thompson H, Edwards D, de Saizieu A, Sparks C, Thompson JA, Greenland AJ, Eyers M, Schuch W (1992) Construction and characterization of yeast artificial chromosome library containing three haploid maize genome equivalents. Plant Mol Biol 19:299–308
Eyers M, Edwards K, Schuch W (1992) Construction and characterisation of a yeast aritifical chromosome library containing two haploid Beta vulgaris L. genome equivalents. Gene 121:195–201
Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Ann Biochem 132:6–13
Giraudat J, Hauge BM, Valon C, Smalle J, Parcy F, Goodman HM (1992) Isolation of the Arabidopsis ABI3 gene by positional cloning. Plant Cell 4:1251–1261
Green ED, Oison MV (1990) Systematic screening of yeast artificialchromosome libraries by use of the polymerase chain reaction. Proc Natl Acad Sci USA 87:1213–1217
Grill E, Somerville C (1991) Construction and characterization of a yeast artifical chormosome library of Arabidopsis which is suitable for chromosome walking. Mol Gen Genet 226:484–490
Guzmán P, Ecker J (1988) Development of large DNA methods for plants: molecular cloning of large segments of Arabidopsis and carrot DNA into yeast. Nuclei Acids Res 16:11091–11105
Heijbroek W, Roelands AJ, de Jong JH (1983) Transfer of resistance to beet cyst nematode from Beta patellaris to sugar beet. Euphytica 32:287–298
Heijbroek W, Roelands AJ, de Jong JH, van Hulst C, Schoone AHL, Munning RG (1988) Sugar beets homozygous for resistance to beet cyst nematode (Heterodera schachtii Schm.) developed from monosomic additions of Beta procumbens to B. vulgaris. Euphytica 38:121–131
Jung C, Herrman RG (1991) A DNA probe for rapid screening of sugar beet (Beta vulgaris L.) carrying extra chromosomes from wild beets of the Procumbentes section. Plant Breed 107:275–279
Jung C, Wricke G (1987) Selection of diploid nematode-resistant sugar beet from monosomic addition lines. Plant Breed 98:205–214
Jung C, Kleine M, Fischer F, Herrmann RG (1990) Analysis of DNA from a Beta procumbens chromosome fragment in sugar beet carrying a gene for nematode resistance. Theor Appl Genet 79:663–672
Jung C, Koch R, Fischer F, Brandes A, Wricke G, Herrmann RG (1992) DNA markers closely linked to nematode resistance genes in sugar beet (Beta vulgaris L.) using chromosome additions and translocations originating from wild beets of the Procumbentes species. Mol Gen Genet 232:271–278
Jung C, Herrmann RG, Eibl C, Kleine M (1995) Molecular analysis of a translocation in sugar beet carrying a gene for nematode resistance from Beta procumbens. J Sugar Beet Res (in press)
Kleine M, Michalek W, Graner A, Herrmann RG, Jung C (1993) Construction of a barley (Hordeum vulgare L.) YAC library and isolation of a Hor 1-specific clone. Mol Gen Genet 240:265–272
Krens F, Zijstra C, van der Molen W, Huizing HJ (1988) Transformation and regeneration in sugar beet (Beta vulgaris L.) induced by “shooter” mutants of Agrobacterium tumefaciens Euphytica 8:185–194
Jant W, Müller J, De Bock TSM (1993) Virulence in the beet cyst nematode (Heterodera schachtii) versus some alien genes for resistance in beet. Fundam Appl Nematol 16:477–454
Lindsey K, Gallois P (1990) Transformation of sugarbeet (Beta vulgaris) by Agrobacterium tumefaciens J Exp Bot 41:529–536
Löptien H (1984) Breeding nematode-resistant beets. II. Investigations into the inheritance of resistance to Herterodera schachtii Schm. in wild species of the section Patellares. Z Pflanzenzuecht 93:237–245
Martin GB, Brommonschenkel SH, Chunwongse J, Frary A, Ganal MW, Spivey R, Wu T, Earle ED, Tanksley SD (1993) Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262:1432–1435
Martin G, Ganal MW, Tanksley SD (1992) Construction of a yeast artifical chromosome library of tomato and identification of cloned segments linked to two diease resistance loci. Mol Gen Genet 233:25–32
Paul H, Van Deelen JEM, Henken B, De Bock TSM, Lange W, Krens W (1990) Expression in vitro of resistance to Heterodera schachtii in hairy roots of an alien monotelosomic addition plant of Beta vulgaris transformed by Agrobacterium rhizogenes Euphytica 48:153–157
Pillen K, Steinrücken G, Herrmann RG, Jung C (1993) An extended linkage map of sugar beet (Beta vulgaris L.) including nine putative lethal genes and the restorer gene X. Plant Breed 11:265–272
Rose MD, Winton F, Hieter P (1990) Methods in yeast genetics. A laboratory manual Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Salentijn EMJ, Sandal NN, Lange W, De Bock TSM, Krens FA, Marcker KA, Stiekema WJ (1992) Isolation of DNA markers linked to a beet cyst nematode resistance locus in Beta patellaris and Beta procumbens. Mol Gen Genet 235:432–440
Savitsky H (1975) Hybridization between Beta vulgaris and Beta procumbens and transmission of nematode (Heterodera schachtii) resistance to sugarbeet. Can J Genet Cytol 17:197–209
Sikorski RS, Hieter P (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19–27
Speckmann GJ, De Bock TSM (1982) The production of alien monosomic additions in Beta vulgaris as a source for the introgression of resistance to beet root nematode (Heterodera schachtii) from Beta species of the section Patellares. Euphytica 31:313–323
Speckmann GJ, De Bock TSM, de Jong JH (1985) Monosomic additions with resistance to beet cyst nematode obtained from hybrids of Beta vulgaris and wild Beta species of the section Patellares. I. Morphology transmission and level of resistance. Z Pflanzenzuecht 95:74–83
Steinumüller K, Apel K (1986) A simple and efficient procedure for isolating plant chromatin which is suitable for studies of DNase I-sensitive domains and hypersensitive sites. Plant Mol Biol 7:87–94
Toxopeus H, Lubberts H (1979) Breeding for resistance to the sugar beet nematode (Heterodera schachtii Schm.) in cruciferous crops. In: EUCARPIA Crucif Conf. PUDOC, Wageningen, the Netherlands, p 151
Ward ER, Jen GC (1990) Isolation of single-copy-sequence clones from a yeast artifical chromosome library of randomly-sheared Arbidopsis thaliana DNA. Plant Mol Biol 14:561–568
Yu MH (1981) Sugar beets homozygous for nematode resistance and their transmission of resistance to their progenies. Crop Sci 21:714–717
Yu MH (1983) Sugarbeet germplasm resistant to sugarbeet nematode. Crop Sci 23:1021–1022
Yu MH (1984) Transmission of nematode resistance in the pedigree of homozygous resistant sugarbeet. Crop Sci 24:88–91
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Kleine, M., Cai, D., Elbl, C. et al. Physical mapping and cloning of a translocation in sugar beet (Beta vulgaris L) carrying a gene for nematode (Heterodera schachtii) resistance from B. procumbens . Theoret. Appl. Genetics 90, 399–406 (1995). https://doi.org/10.1007/BF00221982
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00221982