Abstract
The mdm1 locus on the short arm of chromosome six confers resistance in maize to five strains of the maize dwarf mosaic virus (MDMV), an aphid transmitted potyvirus. The location of mdm1 in relation to RFLP and morphological loci on the short arm of chromosome six was determined using BC1 and F2 mapping populations. The following map order and distance in cM was obtained from the F2 population; jc1270-2.5-npi245-1.6-umc85/po1-0.5-mdm1/nor-0.5-bnl6.29A-0.5-npi235-0.8-npi101A-4.3-numc59. No recombination between mdm1 and the nucleolus organizer region (nor) was detected, as determined using a probe from the intergenic spacer region of the rDNA repeat. In order to resolve the relationship between mdm1 and the nor, and to recover recombinants around mdm1, a highresolution map within the polymitotic1 (po1) yellow kernel1 (y1) interval was generated using [po1 y1 tester (po1 mdm1 y1) x Pa405 (Po1 Mdm1 Y1)] F2 plants. The recessive po1 allele imparts a male-sterile phenotype when homozygous and since po1 and y1 are closely linked, the majority of fertile plants from white endosperm (y1/y1) F2 kernels will arise though a recombination event between the Pa405 Po1 allele and the y1 allele of the po1 y1 tester. Plants from 7,650 white (y1/y1) F2 kernels were examined (15,300 chromosomes) and a total of 626 F2∶3 recombinant families was recovered. Analysis of these recombinants revealed that mdm1 cosegregates with the nor. This lack of recombination between mdm1 and the nor suggests that: either (1) mdm1 is located in the region flanking the nor and recombination is suppressed within that region, or (2) mdm1 is located within the nor.
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References
Agarwal ML, Aldrich J, Agarwal A, Cullis CA (1992) The flax ribosomal RNA-encoding genes are arranged in tandem at single locus interspersed by “non-rDNA” sequences. Gene 120:151–156
Beckett JB (1982) Locating genes to chromosomes: B-A translocation. In: Sheridan WF (ed) Maize for biological research. University Press, University of North Dakota, Grand Forks, pp 71–74
Brown J, Sundaresan V (1991) A recombination hotspot in the maize A1 intragenic region. Theor Appl Genet 81:185–188
Civardi L, Xia Y, Edwards KJ, Schnable PS, Nikolau BJ (1994) The relationship between genetic and physical distances in the cloned a1-sh2 interval of the Zea mays L. genome. Proc Natl Acad Sci USA 91:8268–8272
Coe EH, Polacco M, Davis G, Byrne PF (1994) Genetic data and genetic mapping: some current perspectives. Maydica 39:47–51
Dooner HK, Kermicle JL (1986) The transposable element Ds affects the pattern of intragenic recombination at the Bz and R loci in maize. Genetics 113:135–143
Edwards K, Thompson H, Edwards D, de Saizieu A, Sparks C, Thompson JA, Greenland AJ, Eyers M, Schuch W (1992) Construction and characterization of a yeast artifical chromosome library containing three haploid maize genome equivalents. Plant Mol Biol 19:299–308
Galbraith D, Harkins K, Maddox J, Auers N, Sharma D, Firoozabody E (1983) Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220:1049–1151
Johal GS, Briggs SP (1992) Reductase activity encoded by the Hm1 disease resistance gene in maize. Science 258:985–987
Jupe ER, Zimmer EA (1990) Unmethylated regions in the intergenic spacer of maize and teosinte ribosomal RNA genes. Plant Mol Biol 14:333–347
Jupe ER, Zimmer EA (1993) DNasel-sensitive and undermethylated rDNA is preferentially expressed in a maize hybrid. Plant Mol Biol 21:805–821
Kempter B, Luppa P, Neumeier D (1991) A short procedure for Southern blotting on neutral and anionic membranes. Trends Genet 7:109–110
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Law MD, Moyer JW, Payne GA (1989) Effect of host resistance on pathogenesis of maize dwarf mosaic virus. Phytopathology 79:757–761
Lei JD, Agrios GN (1986) Mechanisms of resistance in corn to maize dwarf mosaic virus. Phytopathology 76:1034–1040
Louie R (1986) Effects of genotype and inoculation protocols on resistance evaluation of maize to maize dwarf mosaic virus strains. Phytopathology 76:769–773
Louie R, Knoke JK, Reichard DL (1983) Transmission of maize dwarf mosaic virus with solid stream inoculum. Plant Dis 67:1328–1331
Louie R, Findley WR, Knoke JK, McMullen MD (1991) Genetic basis of disease resistance in maize to five maize dwarf mosaic virus strains. Crop Sci 31:14–18
Martin GB, Brommonshenkel SH, Chunwongse J, Frary A, Ganal MW, Spivey R, Wu T, Earle ED, Tanksley SD (1993) Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262:1432–1436
McMullen MD, Louie R (1989) The linkage of molecular markers to a gene controlling the symptom response in maize to maize dwarf mosaic virus. Mol Plant-Microbe Interact 2:309–314
McMullen MD, Hunter B, Phillips RL, Rubenstein I (1986) The structure of the maize ribosomal DNA spacer region. Nucleic Acids Res 14:4953–4968
McMullen MD, Phillips RL, Rubenstein (1991) Molecular analysis of the nucleolus organizer region in maize. In Gupta PK, Tsuchiya T (eds) Chromosome engineering in plants: genetics, breeding, evolution, Part A. Elsevier Science Publishers B.V., Amsterdam, pp 561–567
McMullen MD, Jones MW, Simcox KD, Louie R (1994) Three genes control resistance to wheat streak mosaic virus in the maize inbred Pa405. Theor Appl Genet (in press)
Petty ITD, Edwards MC, Jackson AO (1990) Systemic movement of an RNA plant virus determined by a point substitution in 5′ leader sequence. Proc Natl Acad Sci USA 87:8894–8897
Phillips RL (1978) Molecular cytogenetics of the nucleolus organizer region. In: Waiden DB (ed) Maize breeding and genetics. John Wiley and Sons, New York, pp 711–741
Phillips RL (1993) Cytogenetic manipulation of polymitotic (po). Maydica 38:85–92
Rocheford TR, Osterman JC, Gardiner CO (1990) Variation in the ribosomal DNA intergenic spacer of a maize population massselected for high grain yield. Theor Appl Genet 79:793–800
Rocheford TR (1994) Change in ribosomal DNA intergenic spacerlength composition in maize recurrent selection populations. 1. Analysis of BS13, BSSS, and BSCB1. Theor Appl Genet 88: 541–547
Sarkar KR, Coe EH Jr (1971) Analysis of events leading to heterofertilization in maize. J Hered 62:118–120
Simcox KD, McMullen MD (1993) The use of the “modified dry blot” procedure for RFLP analysis. Maize Genet Coop Newslett 67:116–117
Simcox KD, McMullen MD, Louie MD (1993) Mapping multiple disease resistance genes on the short arm of chromosome six. Maize Genet Coop Newslett 67:117–118
Williams SM, Robbins LG, Cluster PD, Allard RW, Strobeck C (1990) Superstructure of the Drosophila ribosomal gene family. Proc Natl Acad Sci USA 87:3156–3160
Williams SM, Robbins LG (1992) Molecular genetic analysis of Drosophila rDNA arrays. Trends Genet 8:335–340
Williams SM, Kennison JA, Robbins LG, Strobeck C (1989) Reciprocal recombination and the evolution of the ribosomal gene family of Drosophila melanogaster. Genetics 122:617–624
Zehr BE, Wright S (1994) Regions of genomic similarity among four “stiff stalk” lines as measured by multiple restriction enzymes in RFLP analysis. Maize Genet Coop Newslett 68:111–112
Zimmer EA, Jupe ER, Walbot V (1988) Ribosomal gene structure, Variation and inheritance in maize and its ancestors. Genetics 120:1125–1136
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Simcox, K.D., McMullen, M.D. & Louie, R. Co-segregation of the maize dwarf mosaic virus resistance gene, Mdm1, with the nucleolus organizer region in maize. Theoret. Appl. Genetics 90, 341–346 (1995). https://doi.org/10.1007/BF00221975
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DOI: https://doi.org/10.1007/BF00221975