Abstract
We examined intraspecific chloroplast (cp) DNA variation within Populus deltoides, P. nigra, and P. maximowiczii by restriction fragment analysis using 16 restriction endonucleases and six heterologous probes of cloned Petunia cpDNA fragments. All three Populus species showed intraspecific cpDNA variation, which was intra- and inter-varietal in P. deltoides, intervarietal in P. nigra, and origin-specific in P. maximowiczii. Two varieties of P. deltoides, var deltoides and var occidentalis, showed distinct cp genomes/DNA. Three distinct cp genomes/DNA, separated by a loss or gain of 1 EcoRV restriction site and/or 1 restriction fragment length polymorphism (RFLP), were observed among the individuals of P. deltoides var deltoides. Within P. nigra, cpDNA of var italica was distinct from that of vars nigra and plantierensis by one RFLP and by a loss or gain of one BamHI restriction site. Populus maximowiczii clones of Chinese origin were separated from those of Japanese origin by a gain or loss of one ClaI restriction site in their cpDNA. The estimate of nucleotide substitutions per site in cpDNA was 0.07% between two varieties of P. deltoides, 0.05% between var italica and var nigra or plantierensis of P. nigra, and 0.01% between Japanese and Chinese accessions of P. maximowiczii.
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Barrett JW, Rajora OP, Yeh FCH, Dancik BP, Strobeck C (1993) Mitochondrial DNA variation and genetic relationships of Populus species. Genome 36:87–93
Brunsfeld SJ, Soltis DE, Soltis PS (1992) Evolutionary patterns and processes in Salix sect. Longifoliae: evidence from chloroplast DNA. Syst Bot 17:239–256
Chiba S (1984) Provenance selection and cross breeding of Populus maximowiczii in northern Japan. Proc Joint Meeting IUFRO Working Parties S2–02–10 Poplar provenances and S2–03–07 Breeding poplar, XVII Session Int Poplar Comm, Ottawa, Canada. Ministry of Works and Development, Palmerston North, New Zealand, pp 79–95
Clegg MT, Zurawski G (1992) Chloroplast DNA and the study of plant phylogeny: present status and future prospects. In: Soltis PS, Soltis DE, Doyle JJ (eds) Molecular systematics of plants. Chapman and Hall, New York, pp 1–13
Crawford DJ (1990) Plant molecular systematics. John Wiley & Sons, New York
Dickmann DI, Stuart KW (1983) The culture of poplars in eastern North America. Michigan State University, East Lansing, Mich.
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Eckenwalder JE (1977) Systematics of Populus L. (Salicaceae) in southwestern North America with special reference to section Aigeiros Duby. PhD thesis, University of California, Berkeley, Calif.
FAO (1958) Poplars in forestry and land use. FAO For For Prod Stud 12. FAO, Rome, Italy
FAO (1979) Poplars and willows in wood production and land use. FAO For Ser 10. FAO, Rome, Italy
Harris SA, Ingram R (1991) Chloroplast DNA and biosystematics: the effects of intraspecific diversity and plastid transmission. Taxon 40:393–412
Maini JS (1968) Silvics and ecology of Populus in Canada. In: Maini JS, Cayford JH (eds) Growth and utilization of poplars in Canada. Can Dep For Branch, Dep Publ No 1205, pp 20–69
Nei M (1987) Molecular evolutionary genetics. Columbia Univeristy Press, New York
Palmer JD (1987) Chloroplast DNA evolution and biosystematic uses of chloroplast DNA variation. Am Nat 130:S6-S29
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) Chloroplast DNA evolution and origin of amphidiploid Brassica species. Theor Appl Genet 65:181–189
Palmer JD, Jansen RK, Michaels HJ, Chase MW, Manhart JR (1988) Chloroplast DNA variation and plant phylogeny. Ann Mo Bot Gard 75:1180–1206
Rajora OP (1988) Allozymes as aids for identification and differentiation of some Populus maximowiczii Henry clonal varieties. Biochem Syst Ecol 16:635–640
Rajora OP (1989a) Genetic structure and identification of Populus deltoides clones based on allozymes. Genome 32:440–448
Rajora OP (1989b) Characterization of 43 Populus nigra L. clones representing selections, cultivars and botanical varieties based on their multilocus allozyme genotypes. Euphytica 43:197–206
Rajora OP, Dancik BP (1992) Chloroplast DNA inheritance in Populus. Theor Appl Genet 84:280–285
Rajora OP, Dancik BP (1995) Chloroplast DNA variation in Populus. II. Interspecific restriction fragment polymorphisms and genetic relationships among Populus deltoides, P. nigra, P. maximowiczii, and P. x canadensis. Theor Appl Genet 90:324–330
Rajora OP, Zsuffa L, Dancik BP (1991) Allozyme and leaf morphological variation of eastern cottonwood at the northern limits of its range in Ontario. For Sci 37: 688–702
Schreiner EJ (1971) Genetics of eastern cottonwood. USDA For Serv Res Pap WO-11
Smith RL, Sytsma KJ (1990) Evolution of Populus nigra (sect. Aigeiros): introgressive hybridization and the chloroplast contribution of Populus alba (sect. Populus). Am J Bot 77:1176–1187
Soltis DE, Soltis PS, Milligan BG (1992) Intraspecific chloroplast DNA variation: systematics and phylogenetic implications. In: Soltis PS, Soltis DE, Doyle JJ (eds). Molecular systematics of plants. Chapman and Hall, New York, pp 117–150
Strauss SH, Bousquet J, Hipkings VD, Hong Y-P (1992) Biochemical and molecular genetic markers in biosystematic studies of forest trees. New For 6:125–158
Sytsma KJ, Gottlieb LD (1986) Chloroplast DNA evolution and phylogenetic relationships in Clarkia sect. Peripetasma (Onagraceae). Evolution 40:1248–1261
Wagner DB (1992) Nuclear, chloroplast, and mitochondrial DNA polymorphisms as biochemical markers in population genetic analyses of forest trees. New For 6:373–390
Zsuffa L (1974) The genetics of Populus nigra L. Ann For 6:29–53
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Communicated by H. F. Linskens
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Rajora, O.P., Dancik, B.P. Chloroplast DNA variation in Populus. I. Intraspecific restriction fragment diversity within Populus deltoides, P. nigra and P. maximowiczii . Theoret. Appl. Genetics 90, 317–323 (1995). https://doi.org/10.1007/BF00221971
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DOI: https://doi.org/10.1007/BF00221971