Abstract
The chromosomal locations of restriction fragments of nuclear DNA that were homologous to four mitochondrial plasmid-like DNAs, namely, B1, B2, B3 and B4, were analyzed by restriction fragment length polymorphism (RFLP) analysis in cultivated rice. Nine kinds of fragments homologous to plasmidlike DNAs were analyzed for their segregation in three different F2 populations derived from intercrosses between rice subspecies; these were found to be localized in three chromosomal regions: three, one and five kinds of nuclear homologues were situated on chromosomes 1,3 and 8, respectively. Nuclear homologues on a given chromosome were tightly linked even though they were homologous to different plasmid-like DNAs. The loci of nuclear homologues found commonly in two or three cultivars were found to be highly conserved, a result that is consistent with their stable transmission. These results and those of the Southern analysis suggest the independent integration of these sequences during the varietal differentiation of rice. The concentration of loci for nuclear homologues on chromosomes 1 and 8 suggests that these sequences were integrated non-randomly into these chromosomal regions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abbot AG, O'Dell M, Flavell RB (1985) Quantitative variation in components of the maize mitochondrial genome between tissues and between plants with different male-sterile cytoplasm. Plant Mol Biol 4:233–240
Allard RW (1956) Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24: 235–278
de la Canal L, Crouzillat D, Flamand MC, Perrault A, Boutry M, Ledoigt G (1991) Nucleotide sequence and transcriptional analysis of a mitochondrial plasmid from a cytoplasmic malesterile line of sunflower. Theor Appl Genet 81:812–818
Crouzillat D, Gentzbittel L, de la Canal L, Vaury C, Perrault A, Nicolas P, Ledoigt G (1989) Properties and nucleotide sequence of a mitochondrial plasmid from sunflower. Curr Genet 15:283–289
Flamand MC, Goblet JP, Duc G, Briquet M, Boutry M (1992) Sequence and transcription analysis of mitochondrial plasmids isolated from cytoplasmic male-sterile lines of Vicia faba. Plant Mol Biol 19:913–923
Fukuchi M, Shikanai T, Kossykh VG, Yamada Y (1991) Analysis of nuclear sequences homologous to the B4 plasmid-like DNA of rice mitochondria; evidence for sequence transfer from mitochondria to nuclei. Curr Genet 20:487–494
Kanazawa A, Sakamoto W, Kishimoto N, Yano M, Tsutsumi N, Saito A, Tano S (1991) Linkage analysis of the nuclear homologues of mitochondrial plasmid-like DNAs in rice. Jpn J Genet 66:597–607
Kanazawa A, Sakamoto W, Nakagahra M, Kadowaki K, Tsutsumi N, Tano S (1992) Distribution and quantitative variation of mitochondrial plasmid-like DNAs in rice (Oryza sativa L.). Jpn J Genet 67:309–319
Kemble RJ, Mans RJ, Gabay-Laughnan S, Laughnan JR (1983) Sequences homologous to episomal mitochondrial DNA in the maize nuclear genome. Nature 304:744–747
Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175
Murray T, Thompson WS (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325
Nakagahra M (1977) Genetic analysis for esterase isozymes in rice cultivars. Jpn J Breed 4:1–14
Pring DR, Lonsdale DM (1985) Molecular biology of higher plant mitochondrial DNA. Int Rev Cytol 97:1–46
Saito A, Yano M, Kishimoto N, Nakagahra M, Yoshimura A, Saito K, Kuhara S, Ukai Y, Kawase M, Nagamine T, Yoshimura S, Ideta O, Ohsawa R, Hayano Y, Iwata N, Sugiura M (1991) Linkage map of restriction fragment length polymorphisms loci in rice. Jpn J Breed 41:665–670
Sakamoto W, Momose M, Tsutsumi N, Tano S, Yamaguchi H (1989) Analysis of homology of small plasmid-like mitochondrial DNAs in the different cytoplasmic male sterile strains in rice. Jpn J Genet 64:49–56
Sakamoto W, Kadowaki K, Tano S, Yabuno T (1990) Analysis of mitochondrial DNAs from Oryza glaberrima and cytoplasmic substituted line for Oryza sativa associated with cytoplasmic male sterility. Jpn J Genet 65:1–6
Sakamoto W, Kadowaki K, Kishimoto N, Yano M, Saito A, Tano S (1991) RFLP analysis of nuclear DNAs homologous with mitochondrial plasmid-like DNAs in cultivated rice. Theor Appl Genet 82:179–184
Schuster W, Brennicke A (1988) Interorganellar sequence transfer: plant mitochondrial DNA is nuclear, is plastid, is mitochondrial. Plant Sci 54:1–10
Shay JW, Werbin H (1992) New evidence for the insertion of mitochondrial DNA into the human genome: significance for cancer and aging. Mutat Res 275:227–235
Shikanai T, Yamada Y (1988) Properties of the circular plasmidlike DNA, B4, from mitochondria of cytoplasmic male-sterile rice. Curr Genet 13:441–443
Shikanai T, Yang ZQ, Yamada Y (1987) Properties of the circular plasmid-like DNA B1 from mitochondria of cytoplasmic male-sterile rice. Plant Cell Physiol 28:1243–1251
Shikanai T, Yang ZQ, Yamada Y (1989) Nucleotide sequence and molecular characterization of plasmid-like DNAs from mitochondria of cytoplasmic male-sterile rice. Curr Genet 15:349–354
Smith AG, Pring DR (1987) Nucleotide sequence and molecular characterization of a maize mitochondrial plasmid-like DNA. Curr Genet 12:617–623
Thorsness PE, Fox TD (1990) Escape of DNA from mitochondria to the nucleus in Saccharomyces cerevisiae. Nature 346:376–379
Ukai Y, Ohsawa R, Saito A (1990) Automatic determination of the order of RFLPs in a linkage group by multi-dimensional scaling method. Jpn J Breed 40 [Suppl 2]: 302–303
Yamaguchi H, Kakiuchi H (1983) Electrophoretic analysis of mitochondrial DNA from normal and male sterile cytoplasms in rice. Jpn J Genet 58:607–611
Author information
Authors and Affiliations
Additional information
Communicated by G. S. Khush
Rights and permissions
About this article
Cite this article
Kanazawa, A., Kishimoto, N., Sakamoto, W. et al. Restriction fragments homologous to mitochondrial plasmid-like DNAs are located within limited chromosomal regions on the rice nuclear genome. Theoret. Appl. Genetics 87, 577–586 (1993). https://doi.org/10.1007/BF00221881
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00221881