Abstract
Twenty fusants of S. Jacobaea L. (chlorophyll deficient) (x) fuchsii Gmel. (wild-type) were investigated by studying chromosome number, isozymes, and restriction fragment length polymorphism (RFLP). Sublines were established from primary shoots, and clones were grown from isolated protoplasts. Out of the 12 hybrid lines 9 had the amphidiploid chromosome number of 80 (2x = 40) as concluded from each one subline. Three hybrid lines showed reduced chromosome numbers between 60 and 76, indicating karyotypic instability. Additive isozyme complements with peroxidase, esterase, malate dehydrogenase, and glutamate-oxalacetate transaminase activities were found. In the RFLP analyses, three ptDNA probes were able to demonstrate the origin of plastomes from either one or both parents, but singular chondriomes from either parent were traced with a mtDNA probe. The results of detailed studies with sublines and clones of two hybrid lines revealed that the chlorophylldeficiency mutation of one parental S. jacobaea clone is located in the genome and that of the other one is located in the plastome. Eight fusant lines were putative cybrids. Mutant plastids were not transmitted into the regenerated putative cybrid shoots. The mtDNA of S. jacobaea alone was detected with a mtDNA probe in seven putative cybrid lines; in one line mtDNA fragments of both the parents were traced. In three lines, the nucleus and the plastids only showed S. fuchsii characters, but the mtDNA was of S. jacobaea.
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Abbreviations
- χ :
-
Chondriome
- mtDNA:
-
mitochondrial DNA
- π :
-
plastome
- ptDNA:
-
plastid DN A
- S.f or f :
-
Senecio fuchsii
- S.j or j :
-
Senecio jacobaea
References
Al-Atabee JS, Mulligan BJ, Power JB (1990) Interspecific somatic hybrids of Rudbeckia hirta and R. laciniata (Compositae). Plant Cell Rep 8:517–520
Allen RC, Saravis CA, Maurer HR (1984) Gel electrophoresis and isoelectric focusing of proteins, selected techniques. Walter de Gruyter, Berlin, New York
Aviv D, Galun E (1985) An in vitro procedure to assign pigment mutations in Nicotiana to either the chloroplast or the nucleus. Hered 76:135–136
Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281:401–403
Binding H, Nehls R (1980) Protoplast regeneration to plants in Senecio vulgaris L. Z Pflanzenphysiol 99:183–185
Binding H, Nehls R, Kock R, Finger J, Mordhorst G (1981) Comparative studies on protoplast regeneration in herbaceous species of the Dicotyledoneae class. Z Pflanzenphysiol 101:119–130
Binding H, Krumbiegel-Schroeren G, Nehls R (1986) Protoplast fusion and early development of fusants. In: Reinert J, Binding H (eds) Results and problems in cell differentiation 12-differentiation of protoplasts and transformed cells, Springer, Berlin Heidelberg New York, pp 37–66
Binding H, Görschen E, Jörgensen J, Krumbiegel-Schroeren G, Ling HQ, Rudnick J, Sauer A, Zuba M, Mordhorst G (1988a) Protoplast culture in agarose media with particular emphasis to streaky culture lenses. Bot Acta 101:233–239
Binding H, Zuba M, Rudnick J, Mordhorst G (1988b) Protoplast gel fusion. J Plant Physiol 133:409–413
Binding H, Bünning D, Görschen E, Hansen U-P, Ling HQ, Röhl S, Zuba M. (1989) Protoplast plating and fusion in agarose lenses applied to potato. Vortr Pflanzenzuecht 15:18–10
Binding H, Görschen E, Hassanein AM, Ling HQ, Mordhorst G, Puck G, Rudnick J, Wang GR, Truberg B (1992a) Plant development from protoplasts of members of Bryophyta, Pteridophyta and Spermatophyta under identical conditions. Physiol Plant 85:295–300
Binding H, Lee DW, Ling HQ, Buch G, Tank G, Rudnick J, Wang GR (1992b) Interspecific interactions in the regeneration from protoplasts. In: Oono K, Hirabayashi T, Kikuch S, Handa H, Kajiwara K (eds) Plant tissue culture and gene manipulation for breeding and formation of phytochemicals. NIAR, Japan, pp 27–34
Brewer GJ (1970) An introduction to isozyme techniques. Academic Press, New York, San Francisco, London
Dudits D, Fejer O, Hadlaczky G, Koncz L, Lázár GB, Horwarth G (1980) Intergeneric gene transfer mediated by plant protoplast fusion. Mol Gen Genet 179:283–288
Falk J, Schmidt A, Krupinska K (1993) Characterization of plastid DNA transcription in ribosome deficient plastids of heat-bleached barley leaves. J Plant Physiol 141:176–181
Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
Gabriel O (1971) Locating enzymes on gels. In: Jakoby WB (ed) Enzyme purification and related techniques, (Methods in enzymology, vol 22.) Academic Press, New York, London, pp 578–604
Gleba YY, Sytnik KM (1984) Protoplast fusion. Springer, Berlin Heidelberg New York Tokyo
Gupta PP, Schieder O, Gupta M (1984) Intergeneric nuclear gene transfer between somatically and sexually incompatible plants through asymmetric protoplast fusion. Mol Gen Genet 197:30–35
Krupinska K (1992) Transcriptional control of plastid gene expression during development of primary foliage leaves of barley grown under a daily light-dark regime. Planta 186:294–303
Krupinska K, Berry-Lowe S (1988) Characterization and in vitro expression of the cytochrome b-559 genes of barley. I. Localization and sequence of the genes. Carlsberg Res Commun 53:43–55
Matsumoto E (1991) Interspecific somatic hybridization between lettuce (Lactuca sativa) and wild species L. virosa. Plant Cell Rep 9:531–534
Medgyesy P, Fejes E, Maliga P (1985) Interspecific chloroplast recombination in a Nicotiana somatic hybrid. Proc Natl Acad Sci USA 82:6960–6964
Mettler IJ (1987) A simple and rapid method for minipreparation of DNA from tissue-cultured plant cells. Plant Mol Biol Rep 51:346–349
Morgan A, Maliga P (1987) Rapid chloroplast segregation and recombination of mitochondrial DNA in Brassica cybrids. Mol Gen Genet 209:240–246
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tissue cultures. Physiol Plant 15:473–497
Power JB, Frearson EM, Hayward C, Cocking EC (1975) Some consequences of the fusion and selective culture of Petunia and Parthenocissus protoplasts. Plant Sci Lett 5: 197–207
Rambaud C, Dubois J, Vasseur J, Lejeune B, Quetier F (1990) Obtention de chicorées males-stériles (Cichorium intybus L. var ‘Magdebourg’) par fusion de protoplastes. Colloq INRA Paris 51:315–316
Rogers SO, Bendich AJ (1988) Extraction of DNA from plant tissue. In: Gelvin SB, Schilperoort RA, Verma DPS (eds) Plant molecular biology manual. Kluwer Academic Publ, Dordrecht Boston London, A6:1–10
Thanh ND, Medgyesy P (1989) Limited chloroplast gene transfer via recombination overcomes plastome-genome incompatibility between Nicotiana tabacum and Solanum tuberosum. Plant Mol Biol 12:87–93
Wang GR, Binding H (1993) Somatic hybridization between Senecio fuchsii Gmel. and S. jacobaea L. Acta Hortic 336:315–320
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Communicated by G. Wenzel
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Wang, G.R., Binding, H. Somatic hybrids and cybrids of Senecio fuchsii Gmel. (x) jacobaea L.. Theoret. Appl. Genetics 87, 561–567 (1993). https://doi.org/10.1007/BF00221879
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DOI: https://doi.org/10.1007/BF00221879