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Genome organization of Magnaporthe grisea: genetic map, electrophoretic karyotype, and occurrence of repeated DNAs

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Abstract

A genetic map of Magnaporthe grisea (anamorph=Pyricularia oryzae and P. grisea), the causal agent of rice blast disease, was generated from segregation data utilizing 97 RFLP markers, two isoenzyme loci and the mating type locus among progeny of a cross between parental strains Guy 11 and 2539. Of the seven chromosomes of M. Grisea, three were resolved by contour-clamped homogeneous electric field (CHEF) electrophoresis, while the remaining four migrated as two doublet bands. By utilizing differences between CHEF mobilities of unresolved chromosomes from the parental strains, Southern analysis with selected markers allowed the chromosomal assignment of all linkage groups. A small translocation involving 1 marker was found in the parental strains used to produce the segregating population from which the map was constructed. Nine classes of repetitive DNA elements were found in the genome of a fungal isolate pathogenic to rice. These occurred only a few times or not at all in the genomes of isolates showing reduced virulence on rice. One repetitive DNA was shown to have structural similarity to the Alu sequences found in primates, a sequence similarity to the copia-like elements of Drosophila, and peptide similarity to transposable elements found in Drosophila, other fungi, and higher plants.

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Author notes

  1. D. Z. Skinner

    Present address: Department of Agronomy and USDA-ARS, Kansas State University, 66506, Manhattan, KS, USA

  2. H. Leung

    Present address: Department of Plant Pathology, Washington State University, 99164, Pullman, WA, USA

Authors and Affiliations

  1. Department of Plant Pathology, University of Wisconsin, 1630 Linden Drive, 53706, Madison, WI, USA

    D. Z. Skinner, M. L. Farman, H. Leung & S. A. Leong

  2. USDA-ARS Plant Disease Resistance Research Unit, 1630 Linden Drive, 53706, Madison, WI, USA

    A. D. Budde, J. R. Smith & S. A. Leong

Authors
  1. D. Z. Skinner
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  2. A. D. Budde
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  3. M. L. Farman
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  4. J. R. Smith
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  5. H. Leung
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  6. S. A. Leong
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Communicated by D. R. Pring

The mention of a trademark, proprietary product, or vendor anywhere in this paper does not constitute a guarantee or warranty of the product by the USDA-ARS and does not imply its approval to the exclusion of other products or vendors that also may be suitable

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Skinner, D.Z., Budde, A.D., Farman, M.L. et al. Genome organization of Magnaporthe grisea: genetic map, electrophoretic karyotype, and occurrence of repeated DNAs. Theoret. Appl. Genetics 87, 545–557 (1993). https://doi.org/10.1007/BF00221877

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  • DOI: https://doi.org/10.1007/BF00221877

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