Abstract
Two novel 46-chromosome doubled haploid lines, W66 and M17, derived from separate hexaploid triticale x bread wheat crosses, were characterised using cytological and biochemical markers. Both lines were shown to be relatively stable cytologically, over 11 and 8 generations of selfing, respectively. By examining mitotic and meiotic chromosomes, the stabilities of the two lines were shown to be similar with frequencies of 2n=46 in 74.2–85.5% of cells. However, over selfed generations, the rye chromosomes were shown to have lost some of their heterochromatin, which made it difficult to establish their continued presence using cytological techniques, such as C-banding alone. Cytological evidence from pairing studies, C-banding, and fluorescence in-situ hybridization, showed that both M17 and W66 are wheat/rye multi-addition lines with rye chromosome constitutions of 1R+6R, and 1R+4R, respectively. These conclusions were confirmed by isozyme and storage-protein analysis.
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Communicated by G. Wenzel
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Wang, G., Ji, J., Wang, YB. et al. The genetic characterisation of novel multi-addition doubled haploid lines derived from triticale x wheat hybrids. Theoret. Appl. Genetics 87, 531–536 (1993). https://doi.org/10.1007/BF00221875
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DOI: https://doi.org/10.1007/BF00221875