Skip to main content
Log in

Electron microscope study on the occurrence of electron-dense deposits at the cell membrane of chicken erythrocytes

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

Electron-dense deposits were found along the inner side of the cell membrane of chicken erythrocytes when fixed in a CaCl2-containing glutaraldehyde solution. Osteoblasts and osteoclasts were studied in an attempt to determine whether the deposits are related to calcium metabolism. No electron-dense deposits adjacent to the cell membrane were found in either these cells or in rat erythrocytes. Therefore, it is assumed that the deposits are not calcium-binding protein necessary for the cellular calcium uptake.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bone, Q., Denton, E. J.: The osmotic effects of electron microscope fixatives. J. Cell Biol. 49, 571–581 (1971)

    Google Scholar 

  • Emtage, J. S., Lawson, D. E. M., Kodicek, E.: Vitamin D-induced synthesis of mRNA for calcium-binding protein. Nature (Lond.) 246, 100–101 (1973)

    Google Scholar 

  • Hillman, D. E., Llinás, R.: Calcium-containing electron-dense structures in the axons of the squid giant synapse. J. Cell Biol. 61, 146–155 (1974)

    Google Scholar 

  • Huxley, H. E., Zubay, G.: Preferential staining of nucleic acid-containing structures for electron microscopy. J. biophys. biochem. Cytol. 11, 273–296 (1961)

    Google Scholar 

  • Kellenberger, E., Ryter, A., Séchaud, J.: Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J. biophys. biochem. Cytol. 4, 671–678 (1958)

    Google Scholar 

  • Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961)

    Google Scholar 

  • Luk, S. C., Nopajaroonsri, C., Simon, G. T.: The ultrastructure of endosteum: A topographic study in young adult rabbits. J. Ultrastruct. Res. 46, 165–183 (1974a)

    Google Scholar 

  • Luk, S. C., Nopajaroonsri, C., Simon, G. T.: The ultrastructure of cortical bone in young adult rabbits. J. Ultrastruct. Res. 46, 184–205 (1974b)

    Google Scholar 

  • Omdahl, J., Holick, M., Suda, T., Tanaka, Y., De Luca, H. F.: Biological activity of 1,25-dihydroxycholecalciferol. Biochemistry 10, 2935–2940 (1971)

    Google Scholar 

  • Oschman, J. L., Hall, T. A., Peters, P. D., Wall, B. J.: Association of calcium with membranes of squid giant axon. Ultrastructure and microprobe analysis. J. Cell Biol. 61, 156–165 (1974)

    Google Scholar 

  • Oschman, J. L., Wall, B. J.: Calcium binding to intestinal membranes. J. Cell Biol. 55, 58–73 (1972)

    Google Scholar 

  • Stanka, P.: Zur Interpretation der elektronenmikroskopischen Abbildung von Zytomembranen im Dünnschnitt. I. Theoretische Betrachtung. Mikroskopie 29, 20–26 (1973)

    Google Scholar 

  • Stanka, P.: Zur Interpretation der elektronenmikroskopischen Abbildung von Zytomembranen im Dünnschnitt. II. Experimentelle Prüfung. Mikroskopie 30, 91–94 (1974)

    Google Scholar 

  • Venable, J. H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 25, 407–408 (1965)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Supported by the Deutsche Forschungsgemeinschaft.

The author is indebted to Professor R. Schauer for valuable discussions.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stanka, P. Electron microscope study on the occurrence of electron-dense deposits at the cell membrane of chicken erythrocytes. Cell Tissue Res. 156, 223–228 (1975). https://doi.org/10.1007/BF00221805

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00221805

Keywords

Navigation