Summary
The mechanism for acidification of zymogen granules in acinar cells of mouse parotid gland was explored using acridine orange, lysosomotropic agents, and an inhibitor of cellular ATP production. Methylamine and monensin reversibly collapsed the pH gradient of granules without affecting cellular ATP levels. Depletion of cellular ATP with antimycin A did not collapse the pH gradient. However, recovery of acidity in the granules, after collapse of the pH gradient by methylamine, was blocked by depletion of cellular ATP. These results demonstrate that zymogen granules of parotid gland are acidic in situ and that ATP is required for acidification of the granules.
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References
Arvan P, Rudnick G, Castle JD (1984) Osmotic properties and internal pH of isolated rat parotid secretory granules. J Biol Chem 259:13567–13572
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
De Lisle RC (1984) Electrolyte Transport Properties of Pancreatic Zymogen Granules (PhD Thesis). Cleveland, OH: Case Western Reserve University
De Lisle RC, Hopfer U (1986) Electrolyte transport properties of pancreatic zymogen granules: implications for pancreatic secretion. Am J Physiol 250:G489-G496
De Lisle RC, Williams JA (1987) Zymogen granule acidity is not required for stimulated pancreatic protein secretion. Am J Physiol 253:G711-G719
Kimmich GA, Randles J, Brand JS (1975). Assay of picomole amounts of ATP, ADP and AMP using the luciferase enzyme system. Anal Biochem 69:187–206
Lee HC, Forte JG, Epel D (1982) The use of fluorescent amines for the measurement of pHi: Applications in liposomes, gastric microsomes, and sea urchin gametes. In: Deamer DW (ed) Intracellular pH: Its Measurement, Regulation, and Utilization in Cellular Functions. A Liss, New York, pp 135–160
Niederau C, Van Dyke RW, Scharschmidt BF, Grendell JH (1986) Rat pancreatic zymogen granules: an actively acidified compartment. Gastroenterology 91:1433–1442
Orci L, Ravazzola M, Anderson RGW (1987) The condensing vacuole of exocrine cells is more acidic than the mature secretory granule. Nature 326:77–79
Rudnick G (1986) ATP-driven H+ pumping into intracellular organelles. Ann Rev Physiol 48:403–413
Williams JA, Korc M, Dormer RL (1978) Action of secretagogues on a new preparation of functionally intact, isolated pancreatic acini. Am J Physiol 235:E517-E524
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De Lisle, R.C., Steinberg, R. & Williams, J.A. Zymogen granules of mouse parotid acinar cells are acidified in situ in an ATP-dependent manner. Cell Tissue Res. 253, 267–269 (1988). https://doi.org/10.1007/BF00221764
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DOI: https://doi.org/10.1007/BF00221764