Summary
The ultrastructure of porcine ventricular tissue was studied by electron microscopy and immunocytochemical techniques. Electron-dense specific granules were found in both Purkinje fibers and transitional cells in the ventricular walls, and were positively stained by the immunogold staining method using an antiserum against atrial natriuretic polypeptide (ANP). This suggests that both the Purkinje fibers and transitional cells display the same specific granules as atrial cardiocytes containing ANP. These results demonstrate that Purkinje fibers and two types of transitional cells, in addition to the ordinary ventricular cardiocytes, can be identified in porcine ventricular wall tissue.
Similar content being viewed by others
References
Alanis J, Benitez D (1970) Rate of rise of Purkinje and transitional cells action potential and the propagation across the Purkinje-myocardium junction. Jp J Phyisol 20:217–232
Back H, Stumpf WE, Ando E, Nokihara K, Forssmann WG (1986) Immunocytochemical evidence for CDD/ANP-like peptides in strands of myoendocrine cells associated with the ventricular conduction system of the heart. Anat Embryol 175:223–226
Bencosme SA, Berger JM (1971) Specific granules in mammalian and non-mammalian vertebrate cardiocytes. In: Bajusz E, Jasmin G (eds) Functional morphology of the heart. Methods Ach Exp Pathol, Vol 5. Karger, Basel, pp 173–213
Benitez D, Alanis J (1970) Calcium ions and the propagation of impulses through the mamalian heart junctional regions. Jp J Physiol 20:233–249
Berger JM, Rona G (1971) Functional and fine structural heterogeneity of atrial cardiocytes. In: Bajusz E, Jasmin G (eds) Functional morphology of the heart. Methods Arch Exp Pathol, Vol 5, Kagler, Basel, pp 540–590
Bompiani Par GD, Rouilier Ch, Hatt PY (1959) Le tissue de conduction do coeur chez le rat. Étude au microscope electronique. Arch Mal Coeur 52:1257–1274
Cantin M, Gutkowska J, Thibault G, Milne RW, Ledoux S, MinLi S, Chapiau C, Garcia R, Harnet P, Genest J (1984) Immunocytochemical localization of atrial natriuretic factor in the heart and salivary glands. Histochemistry 80:113–127
Challice CE (1971) Functional morphology of the specialized tissues of the heart. In: Bajusz E, Jasmin G (eds) Methods Ach Exp Pathol, Vol 5, Karger, Basel, pp 121–172
Challice CE, Virágh Sz (1973) The embryonic development of the mammalian heart. In: Challice CE, Virágh Sz (eds) Ultrastructure of the mammalian heart. Academic Press, New York, London, pp 91–126
Forsgren S, Carlsson E, Strehler E, Thornell L (1982) Ultrastructural identification of human fetal Purkinje fibers — a comparative immunocytochemical and electron microscopic study of composition and structure of myofibrillar M-regions. J Mol Cell Cardiol 14:437–449
Forssmann WG, Birr C, Carlquist M, Christmann M, Finke R, Henschen A, Hock D, Kirchheim H, Kreye V, Lotspeich F, Metz J, Mutt V, Reinecke M (1984) The auricular myocardiocytes of the heart constitute an endocrine organ. Characterization of a porcine cardiac peptide hormon, cardiodilatin-126. Cell Tissue Res 238:425–430
Gardner DC, Dechepper CF, Ganong WF, Hane S, Fiddes J, Baxter JD, Lewicki J (1986) Extra-atrial expression of the gene for atrial natriuretic fator. Biochemistry 83:6697–6701
Guesdon J, Ternynck T, Avrameas S (1979) The use of avidinbiotin interaction in immunoemzymatic techniques. J Histochem Cytochem 27:1131–1139
Horisberger M (1979) Evaluation of colloidal gold as a cytochemical marker for transmission and scanning electron microscopy. Biol Cell 36:253–258
Horisberger M (1981) Colloidal gold: A cytochemical marker for light and fluorescent microscopy and for trasmission and scanning electron microsocpy. Scan Electron Microsc 2:9–31
Hsu S, Raine L, Fanger H (1981) Use of avidin-biotin peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580
Jamieson JD, Palade GE (1964) Specific granules in atrial muscle cells. J Cell Biol 23:151–172
Kaiho M, Anzai M, Kukaida M, Ishiyama I (1986) Heterogeneity in the myoglobin content of chicken heart Purkinje fibers. Histochemistry 84:151–153
Kisch B (1965) Further studies about the microbodies in the atria of the heart. Exp Med Surg 23:1–12
Kangawa K, Matsuo H (1984) Purification and complete amino acid sequence of α-human atrial natriuretic polypeptide (α-hANP). Biochem Biophys Res Commun 118:131–139
Kangawa K, Fukuda A, Kubota I, Hayashi Y, Matsuo H (1984) Identification in rat atrial tissue of multiple forms of natriuretic polypeptides of about 3000 daltons. Biochem Biophys Res Commun 121:585–592
Kangawa K, Fukuda A, Matsuo H (1985) Structural identification of β- and γ-human atrial natriueretic polypeptides. Nature 313:31, 397–400
Metz J, Mutt V, Forssmann WG (1984) Immunohistochemical localization of cardiodilatin in myoendocrine cells of the cardiac atria. Anat Embryol 170:123–127
Miyata A, Kangawa K, Toshimori T, Hatoh T, Matsuo H (1985) Molecular forms of atrial natriuretic polypeptides in mammalian tissue and plasma. Biochem Biophys Res Commun 129:248–255
Miyata A, Kangawa K, Toshimori T, Matsuda H, Hatoh T, Matsuo H (1986) Molecular forms and distribution of atrial natriuretic polypeptides (ANPs) in rat tissues and plasma. Peptide Chemistry 1985: Y Kiso (ed) Protein Research Foundation. Osaka pp 17–22
Morinaga S, Tsumuraya M, Nakajima T, Shimosato Y, Yoshinaga K, Yamaguchi K, Abe K, Ohno H, Kanai A (1985) Immunohistochemical and immunocytochemical localization of atrial natriuretic polypeptide in human adult and fetal hearts. Acta Histochem Cytochem 18:605–613
Palomo AM, Alanis J, Bentez D (1970) Transitional cardiac cells of the conduction system of the dog heart. Distinguishing morphological and electrophysiological features. J Cell Biol 47:1–17
Saper CB, Standaert DG, Currie MG, Schwartz D, Geller DM, Needleman P (1985) Atriopeptin-immunoreactive neurons in the brain: Presence in cardiovascular regulatory areas. Science 227:1047–1049
Shimada T, Nakamura M, Notohara A (1983) The Purkinje fibermyocardial cell region in the goat heart as studies by combined scanning electron microscopy and chemical digestion. Experientia 40:849–850
Sommer JR, Johnson EA (1968) Cardiac muscle. A comparative study of Purkinje fibers and ventricular fibers. J Cell Biol 36:497–526
Thornell LE, Eriksson A (1981) Filament systems in the Purkinje fibers of the heart. Am J Phyisol 241:H291-H305
Toshimori H, Toshimori K, Oura C, Matsuo H (1987a) Immunohistochemical study of atrial natriuretic polypeptides in the embryonic, fetal, and neonatal rat heart. Cell Tissue Res 248:627–633
Toshimori H, Toshimori K, Ōura C, Matsuo H (1987b) Immunohistochemistry and immunocytochemistry of atrial natriuretic polypeptide in porcine heart. Histochemistry 86:595–601
Toshimori H, Toshimori K, Ōura C, Matsuo H, Matsukura S (1987c) The distribution of atrial natriuretic polypeptide (ANP)-containing cells in the adult rat heart. Anat Embryol (in press)
Virágh S, Challice CE (1973) The impulse generation and conduction system of the heart. In: Virágh S, Challice CE (eds) Ultrastructure of the mammalian heart. Academic Press, New York, London, pp 43–89
Virágh S, Porte (1973) On the impulse conducting system of the monkey heart (Macaca mulatta). II. The atrio-ventricular node and bundle. Z Zellforsch 145:363–388
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Toshimori, H., Toshimori, K., Ōura, C. et al. Immunohistochemical identification of Purkinje fibers and transitional cells in a terminal portion of the impulse-conducting system of porcine heart. Cell Tissue Res. 253, 47–53 (1988). https://doi.org/10.1007/BF00221738
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00221738