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A PCR-based marker tightly linked to the nematode resistance gene, Mi, in tomato

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A PCR-based codominant marker has been developed which is tightly linked to Mi, a dominant genetic locus in tomato that confers resistance to several species of root-knot nematode. DNA from tomato lines differing in nematode resistance was screened for random amplified polymorphic DNA markers linked to Mi using decamer primers. Several markers were identified. One amplified product, REX-1, obtained using a pair of decamer primers, was present as a dominant marker in all nematode-resistant tomato lines tested. REX-1 was cloned and the DNA sequences of its ends were determined and used to develop 20-mer primers. PCR amplification with the 20-mer primers produced a single amplified band in both susceptible and resistant tomato lines. The amplified bands from susceptible and resistant lines were distinguishable after cleavage with the restriction enzyme Taq I. The linkage of REX-1 to Mi was verified in an F2 population. This marker is more tightly linked to Mi than is Aps-1, the currently-used isozyme marker, and allows screening of germplasm where the linkage between Mi and Aps-1 has been lost. Homozygous and heterozygous individuals can be distinguished and the procedure can be used for rapid, routine screening. The strategy used to obtain REX-1 is applicable to obtaining tightly-linked markers to other genetic loci. Such markers would allow rapid, concurrent screening for the segregation of several loci of interest.

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  • Beek JG van der, Verkerk R, Zabel P, Lindhout P (1992) Mapping strategy for resistance genes in tomato based on RFLPs between cultivars: Cf9 (resistance to Cladosporium fulvum) on chromosome 1. Theor Appl Genet 84:106–112

    Google Scholar 

  • Bolkan HA, Williamson VM, Waters, CM (1987) Use of cellulose-acetate electrophoresis as an alternative to starch-gel electrophoresis for detecting root-knot nematode resistance in tomato. Plant Dis 71:1001–1003

    Google Scholar 

  • Braham WS, Winstead NN (1957) Inheritance of resistance to root-knot nematodes in tomatoes. Proc Am Soc Hort Sci 69:372–377

    Google Scholar 

  • Chumakov I, Rigault P, Guillou S, Ougen P, Billaut A, Guasconi G, Gervy P, LeGall I, Soularue P, Grinas L, Bougueleret L, Bellanne-Chantelot C, Lacroix B, Barillot E, Gesnouin P, Pook S, Vaysseix G, Frelat G, Schmitz A, Sambucy JL, Bosch A, Estivill X, Weissenbach J, Vignal A, Riethman H, Cox D, Patterson D, Gardiner K, Hattori M, Sakaki Y, Ichikawa H, Ohki M, Le Paslier D, Heilig R, Antonariakis S, Cohen D (1992) Continuum of overlapping clones spanning the entire human chromosome 21 q. Nature 359:380–387

    Google Scholar 

  • Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19:1349

    Google Scholar 

  • Gilbert JC, McGuire DC (1956) Inheritance of resistance to severe root-knot from Meloidogyne incognita in commercial type tomatoes. Proc Am Soc Hort Sci 63:437–442

    Google Scholar 

  • Ho J-Y, Weide R, Ma HM, van Wordragen MF, Lambert KN, Koorneef M, Zabel P, Williamson VM (1992) The root-knot nematode resistance gene (Mi) in tomato: construction of a molecular linkage map and identification of dominant cDNA markers in resistant genotypes. The Plant J 2:971–982

    Google Scholar 

  • Klein-Lankhotrs RM, Rietveid P, Machiels B, Verkert R, Weide R, Gebhardt C, Koornneef M, Zabel P (1991a) RFLP markers linked to the root knot nematode resistance gene Mi in tomato. Theor Appl Genet 81:661–667

    Google Scholar 

  • Klein-Lankhorts RM, Vermunt A, Weide R, Liharska T, Zabel P (1991b) Isolation of molecular markers for tomato (L. esculentum) using random amplified polymorphic DNA (RAPD) Theor Appl Genet 83:108–114

    Google Scholar 

  • Laterrot H (1987) Neat-isogenic tomato lines in Moneymaker type with different genes for disease resistances. Tomato Genet Coop Rep Rep 37:91

    Google Scholar 

  • Martin GB, Williams JGK, Tanksley SD (1991) Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines. Proc Natl Acad Sci USA 88:2336–2340

    Google Scholar 

  • Medina-Filho HP, Stevens MA (1980) Tomato breeding for nematode resistance: survey of resistant varieties for horticultural characteristics and genotype of acid phosphatases. Acta Hortic 100:383–391

    Google Scholar 

  • Messeguer R, Ganal M, de Vicente MC, Young ND, Bolkan H, Tanksley SD (1991) High-resolution RFLP map around the root knot nematode resistant gene (Mi) in tomato. Theor Appl Genet 82:529–536

    Google Scholar 

  • Miller JC, Tanksley SD (1990) RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon. Theor Appl Genet 80:437–448

    CAS  Google Scholar 

  • Olson M, Hood L, Cantor C, Botstein D (1989) A common language for physical mapping of the human genome. Science 245:1434–1435

    Google Scholar 

  • Paran I, Michelmore RW (1993) Development of reliable PCR- based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85:985–993

    CAS  Google Scholar 

  • Rick CM, Fobes J (1974) Association of an allozyme with nematode resistance. Tomato Genet Coop Rep 24:25

    Google Scholar 

  • Tanksley SD, Ganal MW, Prince JP, de Vicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Roder MS, Wing RA, Wu W, Young ND (1992) High-density molecular-linkage maps of the tomato and potato genomes. Genetics 132:1141–1161

    CAS  PubMed  Google Scholar 

  • Williams JGK, Kubelik AR, Livak KJ, Rafalsky A, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    CAS  PubMed  Google Scholar 

  • Williamson VM, Colwell G (1991) Acid phosphatase-1 from nematode resistant tomato: isolation and characterization of its gene. Plant Physiol 97:139–146

    Google Scholar 

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Communicated by M. Koornneef

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Williamson, V.M., Ho, J.Y., Wu, F.F. et al. A PCR-based marker tightly linked to the nematode resistance gene, Mi, in tomato. Theoret. Appl. Genetics 87, 757–763 (1994).

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