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The structure and differentiation of granulated metrial gland cells of the pregnant mouse uterus

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Summary

A study was made with the light and electron microscopes of the granulated metrial gland cells of the decidua basalis of the pregnant mouse uterus, up to day 11 of pregnancy. The granulated metrial gland cells are large, up to 50 μ in diameter, mono- or binucleate and the glycogen rich cytoplasm typically contains many large glycoprotein granules which may be up to 5 μ in diameter. Morphological evidence is described in support of a lymphocyte-like cell being the precursor to the granulated metrial gland cell. This differentiation sequence is similar to that already proposed in the rat but differences between the ultrastructure of the mature metrial gland cells of rats and mice were noted.

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References

  • Baker, B.L.: Histochemical variations in the metrial gland of the rat during pregnancy and lactation. Proc. Soc. exp. Biol. (N.Y.) 68, 492–496 (1948)

    Google Scholar 

  • Bernard, O., Ripoche, M.-A., Bennett, D.: Distribution of maternal immunoglobulins in the mouse uterus and embryo in the days after implantation. J. exp. Med. 145, 58–75 (1977)

    Google Scholar 

  • Bloch, S.: Die Glandula myometralis im Uterus der Maus. Acta anat. (Basel) 56, 103–119 (1964)

    Google Scholar 

  • Bridgman, J.: A morphological study of the development of the placenta of the rat. J. Morph. 83, 61–85 (1948)

    Google Scholar 

  • Bruijn, W.C. de: Glycogen, its chemistry and morphological appearance in the electron microscope. 1. A modified OsO4 fixative which selectively contrasts glycogen. J. Ultrastruct. Res. 42, 29–50 (1973)

    Google Scholar 

  • Bulmer, D., Peel, S.: The demonstration of immunoglobulin in the metrial gland cells of the rat placenta. J. Reprod. Fertil. 49, 143–145 (1977)

    Google Scholar 

  • Dickson, A.D., Bulmer, D.: Observations on the origin of metrial gland cells in the rat placenta. J. Anat. (Lond.) 95, 262–273 (1961)

    Google Scholar 

  • Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137A (1965)

  • Korn, E.D.: Introductory workshop: membranes and their association with contractile proteins. In: Cell mobility, Book B (R. Goldman, T. Pollard and J. Rosenbaum, eds.). Cold Spring Harbor Laboratory 1976

  • Larkin, L.H.: Bioassay of metrial gland extracts for relaxin using the mouse interpubic ligament technique. Endocrinology 94, 567–570 (1974)

    Google Scholar 

  • Larkin, L.H., Cardell, R.R.: Differentiation of granulated metrial gland cells in the uterus of the pregnant rat. Amer. J. Anat. 132, 241–258 (1971)

    Google Scholar 

  • Larkin, L.H., Flickinger, C.J.: Ultrastructure of the metrial gland cell in the pregnant rat. Amer. J. Anat. 126, 337–354 (1969)

    Google Scholar 

  • Larkin, L.H., Schultz, R.L.: Histochemical and autoradiographic studies of the formation of the metrial gland in the pregnant rat. Amer. J. Anat. 122, 607–619 (1968)

    Google Scholar 

  • McFarland, W., Heilman, D.H.: Lymphocyte foot appendage: its role in lymphocyte function and in immunological reaction. Nature (Lond.) 205, 887–888 (1965)

    Google Scholar 

  • Palade, G.E.: A study of fixation for electron microscopy. J. exp. Med. 95, 285–297 (1952)

    Google Scholar 

  • Peach, R., Bulmer, D.: Cytochemistry and electron microscopy of the granulated metrial gland cells of the rat placenta. J. Anat. (Lond.) 99, 415–416 (1965)

    Google Scholar 

  • Peel, S., Bulmer, D.: The fine structure of the rat metrial gland in relation to the origin of the granulated cells. J. Anat. (Lond.) 123, 687–696 (1977)

    Google Scholar 

  • Reynolds, E.S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)

    Google Scholar 

  • Rudell, C.L.: Embedding media for 1–2 micron sectioning. 2. Hydroxyethyl methacrylate combined with 2-butoxyethanol. Stain Technol. 42, 253–255 (1967)

    Google Scholar 

  • Selye, H., McKeown, I.: Studies on the physiology of the maternal placenta in the rat. Proc. roy. Soc. B 119, 1–31 (1935)

    Google Scholar 

  • Smith, L.J.: Metrial gland and other glycogen containing cells in the mouse uterus following mating and through implantation of the embryo. Amer. J. Anat. 119, 15–24 (1966)

    Google Scholar 

  • Zucker-Franklin, D.: The ultrastructure of lymphocytes. Seminars in Hemat. 6, 4–27 (1969)

    Google Scholar 

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Stewart, I., Peel, S. The structure and differentiation of granulated metrial gland cells of the pregnant mouse uterus. Cell Tissue Res. 184, 517–527 (1977). https://doi.org/10.1007/BF00220975

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