Summary
The localization of ovine placental lactogen (OPL) was studied using the immunofluorescence method. The placentome sections were treated by an indirect technique with anti-OPL antibodies obtained from rabbits injected with purified hormone. OPL was located in large cells of the monostratified epithelium of chorionic villi. These cells are mono- or binucleated and PAS-positive. The immunological reaction was inhibited by the specific antigen (OPL, 400μg/ml undiluted anti-OPL antiserum) but not by ovine prolactin, bovine growth hormone, human placental lactogen nor by any other polypeptidic hormone tested. Reciprocally, the localization of OPL-secreting cells was unsuccessful with antibodies raised against these control hormones.
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Martal, J., Djiane, J. & Dubois, M.P. Immunofluorescent localization of ovine placental lactogen. Cell Tissue Res. 184, 427–433 (1977). https://doi.org/10.1007/BF00220966
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DOI: https://doi.org/10.1007/BF00220966