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Identification of the most represented repeated motifs in Arabidopsis thaliana microsatellite loci

Theoretical and Applied Genetics volume 91, pages 160–168 (1995)Cite this article

Abstract

The major simple sequence repeats present in the Arabidopsis genome were identified by Southern hybridizations with 49 oligonucleotide probes matching all the possible combinations of motifs up to 4 nucleotides long. The method used allowed us to perform all the hybridizations under the same temperature conditions. A good correlation was observed with the data obtained from database analysis, indicating that the method can be useful for identifying the major classes of microsatellite loci in species for which few or no sequence data are available. AG/CT, AAG/CTT, ATG/CAT and GTG/CAC are the major motifs present in the Arabidopsis genome that can be used as convenient probes to isolate microsatellite loci by screening libraries. AAG/CTT is the more frequent of these motifs, and its relative frequency in Arabidopsis is much higher than averagely found in the plant kingdom. About 8% of the cDNA clones from an immature silique library contains AG/CT, AAG/CTT or ATG/CAT microsatellite loci. Several microsatellite loci were isolated by screening genomic and cDNA libraries. Twenty-six tri-nucleotide loci were PCR amplified from four different ecotypes, and polymorphism was observed for 12 of them; 10 loci showing two alleles and 2 loci showing three alleles.

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Authors and Affiliations

  1. GDR977 BIOMOVE CNRS, Université Blaise-Pascal, 24, Avenue des Landais, 63177, Aubiere Cedex, France

    A. Depeiges, C. Goubely, A. Lenoir, S. Cocherel & G. Picard

  2. URA 565 and GDR 1003 CNRS, Université de Perpignan, 66860, Perpignan Cedex, France

    M. Raynal, F. Grellet & M. Delseny

Authors
  1. A. Depeiges
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  2. C. Goubely
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  3. A. Lenoir
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  4. S. Cocherel
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  5. G. Picard
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  6. M. Raynal
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  7. F. Grellet
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  8. M. Delseny
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Communicated by J. S. Beckmann

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Depeiges, A., Goubely, C., Lenoir, A. et al. Identification of the most represented repeated motifs in Arabidopsis thaliana microsatellite loci. Theoret. Appl. Genetics 91, 160–168 (1995). https://doi.org/10.1007/BF00220873

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  • DOI: https://doi.org/10.1007/BF00220873

Key words

  • Arabidopsis thaliana
  • Microsatellite
  • Polymorphism
  • PCR amplification