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Synthesis of phosphatidyl-dCMP in permeabilized normal human lymphocytes

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Summary

When peripheral lymphocytes stimulated with phytohemagglutinin were permeabilized in vitro, (3H)dCTP acted as a precursor for DNA synthesis, but the formation of a compound soluble in organic solvents could also be demonstrated. The structure of the latter compound was studied analyzing the products formed after alkaline hydrolysis or an enzymatic treatment with nucleotide pyrophosphatase. Both treatments led to the formation of (3H)dCMP. When stimulated lymphocytes were labeled in vivo with (14C)glycerol before permeabilization and ulterior labeling with (3H)dCTP a double labeled compound was obtained. When this compound was submitted to alkaline hydrolysis, (3H)dCMP. and (l4C)glycerol-3-phosphate were obtained. It was concluded that the compound soluble in organic solvents was phosphatidyl-dCMP.

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Abbreviations

PHA:

phytohemagglutinin

PBS:

phosphate buffered saline

MEM:

Eagle's minimal essential medium

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Authors and Affiliations

  1. Instituto de Investigaciones Bioquímicas “Fundación Campomar”, Obligado 2490, Buenos Aires, Argentina

    Estela Medrano

  2. Fundación CIMAE - Centro de Investigaciones Médicas Albert Einstein, Luis Viale 2831, Buenos Aires, Argentina

    José Mordoh

Authors
  1. Estela Medrano
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  2. José Mordoh
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Medrano, E., Mordoh, J. Synthesis of phosphatidyl-dCMP in permeabilized normal human lymphocytes. Mol Cell Biochem 24, 159–165 (1979). https://doi.org/10.1007/BF00220734

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  • DOI: https://doi.org/10.1007/BF00220734

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