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The characteristics of purified HL60 tuftsin receptors

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Summary

The purification and characteristics of purified HL60 tuftsin receptors are described. Purification was accomplished by affinity chromatography similar to that described earlier, wherein a tuftsin analog Thr-LysPro-Pro-Arg, is covalently linked at the Nα group to a solid support. The receptor consists presumably of two subunits approximately 66 KDa and 57 KDa. The dissociation constant of the receptor complex is 4.7 × 10−8 M with 5 × 104 receptors per cell. It can form oligomers with an Mr of about 560 KDa suggesting an octomeric structure, assuming the same number of each subunit is associated.

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Abbreviations

CHAPS:

3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate

DCC:

Dicyclohexylcarbodiimide

DMSO:

Dimethylsulfoxide

DSS:

Disuccinimidylsuberate

FCS:

Fetal Calf Serum

HEPES:

4-(2-hydroxyethyl)-1-piperazineethanesulfonic Acid

MMP:

Macrophage/Monocyte and Polymorphonuclear granulocytes

Mr:

Relative Molecular mass

NaDodSO4 :

Sodium Dodecyl Sulfate

PEG:

Polyethylene Glycol

SP:

Sucrose Phosphate buffer

TFMS:

Trifluoromethanesulfonic Acid

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Authors and Affiliations

  1. Division of Protein Chemistry, Department of Molecular Biology and Microbiology, Tufts University School, of Medicine, Boston, Massachusetts, USA

    Nancy J. Bump, Victor A. Najjar & Judith Reichler

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  1. Nancy J. Bump
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  2. Victor A. Najjar
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  3. Judith Reichler
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Bump, N.J., Najjar, V.A. & Reichler, J. The characteristics of purified HL60 tuftsin receptors. Mol Cell Biochem 92, 77–84 (1990). https://doi.org/10.1007/BF00220722

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  • DOI: https://doi.org/10.1007/BF00220722

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