Abstract
Regeneration of cilia in Tetrahymena cells treated with 1 µg/ml of 4′,6-diamidino-2-phenylindole (DAPI) takes place about 30 min later then in untreated controls. Analysis of RNA isolated at 50-th min of reciliation, that is when the control cells – but not DAPI-treated ones — have just restored their motility, revealed that; −1. Two peaks of polyadenylated RNA were present in DAPI-treated cells as well as in the control ones; −2. The mobility of the polyadenylated RNA was the same in the two compared cell samples; −3. In wheat germ cell-free translational system these poly (A+)-RNA probably directed the synthesis of α-and β-tubulin; −4. The amount of polyadenylated RNA in compared cell samples was different, in DAPI-treated Tetrahymena its amount was estimated at 1.43% vs. 1.89% in control ones.
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Krawczyńska, W., Kludkiewicz, B. Polyadenylated RNA during DAPI-arrested regeneration of Tetrahymena cilia. Mol Cell Biochem 92, 53–60, (1990). https://doi.org/10.1007/BF00220719
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DOI: https://doi.org/10.1007/BF00220719