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Effect of platelet-activating factor (PAF) on sodium calcium exchange in cardiac sarcolemmal vesicles

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Summary

The effects of platelet-activating factor (PAF) on Na+-dependent calcium uptake in myocardial sarcolemmal vesicles were examined in order to clarify its mechanism of inotropic action on the heart. PAF (40 and 20 µM) significantly inhibited Na+-Ca2+ exchange by 61% and 37%, respectively. Both initial rate of exchange and maximal exchange were inhibited. The Km for the reaction was not altered but Vmax was lowered 55% by PAF. Lyso-PAF inhibited Na+-Ca2+ exchange to a similar degree as PAF. CV-3988, a specific PAF receptor antagonist, failed to diminish the inhibitory effect of PAF on Na+-Ca2+ exchange, suggesting that the effect of PAF on Na+-Ca 2+ exchange is not via a receptor mechanism. The passive permeability of sarcolemmal vesicles to Ca2+ was markedly elevated after PAF treatment. However, this effect could not account for the decrease in Na+-Ca2+ exchange. Interestingly, passive Ca2+ binding to cardiac sarcolemma was increased by 40 µM PAF. This study indicates that a depression of Na+-Ca2+ exchange probably does not play a role in the negative inotropic effect of PAF on the myocardium under physiological conditions. Its mechanism of action on Na+-Ca2+ exchange is discussed.

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Authors and Affiliations

  1. Division of Cardiovascular Sciences, St. Boniface General Hospital Research Center, Dept. of Physiology, University of Manitoba, 351 Tache Ave., R2H 2A6, Winnipeg, Canada

    He-Ping Meng, Michael J. B. Kutryk & Grant N. Pierce

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  1. He-Ping Meng
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  2. Michael J. B. Kutryk
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  3. Grant N. Pierce
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Meng, HP., Kutryk, M.J.B. & Pierce, G.N. Effect of platelet-activating factor (PAF) on sodium calcium exchange in cardiac sarcolemmal vesicles. Mol Cell Biochem 92, 45–51 (1990). https://doi.org/10.1007/BF00220718

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  • DOI: https://doi.org/10.1007/BF00220718

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