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Immunochemical approaches to the study of histone H1 and high mobility group chromatin proteins

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Summary

This review is an attempt to summarize all existing data on histone H1 and high mobility group proteins obtained with immunochemical methods. The following issues are treated consecutively: production of specific antisera to these protein groups, antigenic structure of the polypeptide chains, use of antibodies for the identification, the quantitative estimation and the study of the tissue- and species-specificity of the proteins. Special attention is devoted to the studies of the localization of the respective antigens in the cell, the nucleus, the chromosomes and the interphase chromatin. The use of specific antibodies for the elucidation of the role these proteins play in such basic cellular processes as proliferation and differentiation, replication and transcription is also discussed. It becomes clear that the use of immunochemical approaches in the study of specific chromatin proteins both at the level of the protein molecule and at the level of chromatin can be a powerful tool for the resolution of a number of specific problems. The field is very promising and will undoubtedly develop intensely in the nearest future.

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Abbreviations

AB:

antibody, antibodies

AG:

antigen, antigens

AD:

Antigenic Determinant

DNP:

deoxyribonucleoprotein

bp:

base pairs

HMG:

High Mobility Group (proteins)

References

  1. Simpson RT: Structure of the chromatosome, a chromatin particle containing 160 base pairs of DNA and all the histones. Biochemistry 17: 5524–5531, 1978

    Google Scholar 

  2. Igo-Kemenes T, Hörz W, Zachau HG: Chromatin. Ann Rev Biochem 51: 89–121, 1982

    Google Scholar 

  3. Thomas JO: The higher order structure of chromatin and histone H1. J Cell Sci Suppl l: 1–20, 1984

    Google Scholar 

  4. Weintraub H: Assembly and propagation of repressed and derepressed chromosomal states. Cell 42: 705–711, 1985

    Google Scholar 

  5. Einck L, Bustin M: The intracellular distribution and function of the high mobility group chromosomal proteins. Exp Cell Res 156: 295–310, 1985

    Google Scholar 

  6. Von Holt C, Strickland WN, Brandt WF, Strickland MS: More histone structures. FEBS lett 100: 201–218, 1979

    Google Scholar 

  7. Wu RS, Panusz HT, Hatch CL, Bonner WM: Histones and their modifications. CRC Critical Reviews in Biochemistry 20: 201–263, 1986

    Google Scholar 

  8. Hartman PG, Chapman GE, Moss T, Bradbury EM: Studies on the role and mode of operation of the verylysine-rich histone H1 in eukaryotic chromatin. The three structural regions of the histone H1 molecule. Eur J Biochem 77: 45–51, 1977

    Google Scholar 

  9. Allan J, Hartman PG, Crane-Robinson C, Aviles FX: The structure of histone H1 and its location in chromatin. Nature 288: 675–679, 1980

    Google Scholar 

  10. Allan J, Mitchell T, Harborne N, Böhm L, Crane-Robinson C: Roles of H1 domains in determining higher order chromatin structure and H1 location. J Mol Biol 187: 591–601, 1986

    Google Scholar 

  11. Panyim S, Chalkley R: A new histone found only in mammalian tissues with little cell division. Biochem Biophys Res Common 37: 1042–1049, 1969

    Google Scholar 

  12. Neelin JM, Callahan PX, Lamb DC, Murray K: The histones of chicken erythrocyte nuclei. Can J Biochem 42: 1743–1752, 1964

    Google Scholar 

  13. Miki BLA, Neelin JM: The histones from rainbow trout erythrocytes include an erythrocyte-specific histone. Can J Biochem 53: 1158–1169, 1975

    Google Scholar 

  14. Brown GL, Rutledge RG, Neelin JM: Anuran erythrocytes and liver both contain satellite histone H12. Life Sci 28: 2993–2999, 1981

    Google Scholar 

  15. Shay CE, Foster PG, Neelin JM: Immunological relationships among vertebrate lysine-rich histones. Comp Biochem Physiol,

  16. Cary PD, Hines ML, Bradbury EM, Smith BJ, Johns EW: Conformational studies of histone H10 in comparison with histones H1 and H5. Eur J Biochem 120: 371–377, 1981

    Google Scholar 

  17. Johns EW: History, definitions and problems. In: EW Johns (ed) The HMG chromosomal proteins. Academic Press, London etc, 1982, pp 1–7

    Google Scholar 

  18. Mayes ELV: Species and tissue specificity. In: EW Johns (ed) The HMG chromosomal proteins. Academic Press, London etc, 1982, pp 9–40

    Google Scholar 

  19. Walker JM: Primary structure. In: EW Johns (ed) The HMG chromosomal proteins. Academic Press, London etc, 1982, pp 69–87

    Google Scholar 

  20. Cary PD, Turner CH, Mayes E, Crane-Robinson C: Conformation and domain structure of the non-histone chromosomal proteins, HMG1 and 2. Isolation of two folded fragments from HMG1 and 2. Eur J Biochem 131: 367–374, 1983

    Google Scholar 

  21. Bradbury EM: Conformations and interactions. In: EW Johns (ed) The HMG chromosomal proteins. Academic Press, London etc, 1982, pp 89–110

    Google Scholar 

  22. Jackson JB, Rill RL: Circular dichroism, thermal denaturation, and deoxyribonuclease I digestion studies of nucleosomes highly enriched in high mobility group proteins HMG1 and HMG2. Biochemistry 20: 1042–1046, 1981

    Google Scholar 

  23. Weisbrod S, Weintraub H: Isolation of a subclass of nuclear proteins responsible for conferring a DNAse I-sensitive structure on globin chromatin. Proc Natl Acad Sci USA 76: 630–634, 1979

    Google Scholar 

  24. Stollar BD: Serological analysis of histones. In: Meth Cell Biol. Academic Press, New York, 1978, vol 18, pp 105–122

    Google Scholar 

  25. Goldblatt D, Bustin M: Exposure of histone antigenic determinants in chromatin. Biochemistry 14: 1689–1695, 1975

    Google Scholar 

  26. Mura C, Huang PC, Levy DA: Anti-histone KSA antibody from guinea pig. J Immunol 113: 750–755, 1974

    Google Scholar 

  27. Piha RS, Valkonen KH, Leinonen M, Rimpiläinen L: Solid-phase radioimmuno assay for calf liver nuclear H1 histones. Biochem Intern 2: 333–343, 1981

    Google Scholar 

  28. Srebreva L, Zlatanova J: Specificity studies on anti-histone H1 antibodies obtained by different immunization methods. FEBS lett 200: 123–127, 1986

    Google Scholar 

  29. Sotirov N, Johns EW: Rabbit precipitin antibody to chicken erythrocyte histone F2C. J Immunol 109: 686–691, 1972

    Google Scholar 

  30. Mura CV, Mazen A, Neelin JM, Briand G, Sautiere P, Champagne M: Distribution of antigenicity in chicken erythrocyte histone H5. Eur J Biochem 108: 613–620, 1980

    Google Scholar 

  31. Neary BA, Mura CV, Stollar BD: Serological homologies between H1° and H5 include the carboxyl-terminal domain. J Biol Chem 260: 15850–15855, 1985

    Google Scholar 

  32. Bustin M, Stollar BD: Immunological relatedness of thymus and liver Fl histone subfractions. J Biol Chem 248: 3506–3510, 1973

    Google Scholar 

  33. Hekman A, Sluyser M: Antigenic determinants on lysinerich histones. Biochim Biophys Acta 295: 613–620, 1973

    Google Scholar 

  34. Betz JW, Herman CJ: Immunofluorescence of histone H1 in stimulated lymphocytes measured by flow cytophotometry. J Histochem Cytochem 27: 417–425, 1979

    Google Scholar 

  35. Fukuzawa C, Udaka K, Shimura K: Specificity of silkgland H1 histone. Cell Struct Funct 4: 63–66, 1979

    Google Scholar 

  36. Bers EP, Andreeva NB, Gazaryan KG, Kozlov AV, Lipskaya AA, Ivanova SB, Vodopuanova LG: Immunechemical analysis of histones H1 and H5 from pigeon erythroid cells. Biokhimia (russian) 44: 1264–1273, 1979

    Google Scholar 

  37. Moorman AFM, Cossé AA, de Graaf A, Charles R: Limitations in the applicability of the complement-fixation test for the estimation of specificity of histone antisera. FEBS lett 114: 25–29, 1980

    Google Scholar 

  38. Mura CV, Setterfield G, Neelin JM: Immunofluorescent localization of lysine-rich histones in isolated nuclei from adult and embryonic chicken erythrocytes. Can J Biochem 60: 215–223, 1982

    Google Scholar 

  39. Mazen A, De Murcia G, Bernard S, Pouyet J, Champagne M: Localization of histone H5 in the subunit organization of chromatin using immunoelectron microscopy. Eur J Biochem 127: 169–176, 1982

    Google Scholar 

  40. Bustin M, Stollar BD: Antigenic determinants in lysinerich histones. Biochemistry 12: 1124–1129, 1973

    Google Scholar 

  41. Rózalski M, Lafleur L, Ruiz-Carrillo A: Monoclonal antibodies against histone H5. Epitope mapping and binding to chromatin. J Biol Chem 260: 14379–14386, 1985

    Google Scholar 

  42. Hardin JA, Thomas JO: Antibodies to histones in systemic lupus erythematosus: localization of prominent autoantigens on histones H1 and H2B. Proc Natl Acad Sci USA 80: 7410–7414, 1983

    Google Scholar 

  43. Costa O, Tchouatcha-Tchouassom JC, Roux B, Monier JC: Anti-Hl histone antibodies in systemic lupus erythematosus: epitope localization after immunoblotting of chymotrypsin-digested H1. Clin Exp Immunol 63: 608–613, 1986

    Google Scholar 

  44. Gohill J, Fritzler MJ: Antibodies in procainamide-induced and systemic lupus erythematosus bind the C-terminal of histone 1 (H1). Molecular Immunol 24: 275–285, 1987

    Google Scholar 

  45. Russanova VR, Dimitrov SI, Makarov VL, Pashev IG: Accessibility of the globular domain of histones H1 and H5 to antibodies upon folding of chromatin. Eur J Biochem 167: 321–326, 1987

    Google Scholar 

  46. Aviles FJ, Chapman GE, Kneale GG, Crane-Robinson C, Bradbury EM: The conformation of histone H5. Isolation and characterization of the globular segment. Eur J Biochem 88: 363–371, 1978

    Google Scholar 

  47. Yaguchi M, Roy C, Seligy VL: Complete aminoacid sequence of goose erythrocyte H5 histone and the homology between H1 and H5 histones. Biochem Biophys Res Commun 90: 1400–1406, 1979

    Google Scholar 

  48. Mendelson E, Smith BJ, Bustin M: Mapping the binding of monoclonal antibodies to histone H5. Biochemistry 23: 3466–3471, 1984

    Google Scholar 

  49. Frado L-LY, Mura CV, Stollar BD, Woodcock CLF: Mapping of histone H5 sites on nucleosomes using immunoelectron microscopy. J Biol Chem 258: 11984–11990, 1983

    Google Scholar 

  50. Mura CV, Stellar BD: Serological detection of homologies of H1° with H5 and H1 histones. J Biol Chem 256: 9767–9769, 1981

    Google Scholar 

  51. Allan J, Smith BJ, Dunn B, Bustin M: Antibodies against the folding domain of histone H5 cross-react with H1° but not with H1. J Biol Chem 257: 10533–10535, 1982

    Google Scholar 

  52. Yasuda H, Logan KA, Bradbury EM: Antibody against globular domain of H1° histone. FEBS lett 166: 263–266, 1984

    Google Scholar 

  53. Banchev TB, Srebreva LN, Zlatanova JS: The C-terminal fragment of histone H1° contains antigenic determinants. Comp Rend Acad Bulg Sci 41: 83–86, 1988

    Google Scholar 

  54. Vila JLI, Juarez C, Illa I, Agusti M, Gelpi C, Amengual MJ, Rodriguez JM: Autoantibodies against the H1° subtype of histone H1. Clin Immunol Immunopath 45: 499–503, 1987

    Google Scholar 

  55. Neary BA, Stollar BD: The carboxyl-terminal domain of murine H1°. Immunochemical and partial amino acid sequence comparisons with other H1°/H1/H5 histones. Eur J Biochem 168: 161–167, 1987

    Google Scholar 

  56. Markose ER, Rao MRS: Testis-specific histone H1t is antigenically distinct among H1 subtypes. J Biol Chem 260: 16263–16268, 1985

    Google Scholar 

  57. Bustin M, Dunn B, Gillette R, Mendelson E, Scares N: Antigenic determinants of high mobility group chromosomal proteins 1 and 2. Biochemistry 21: 6773–6777, 1982

    Google Scholar 

  58. Vanderbilt JN, Anderson JN: Monoclonal antibodies as probes for the complexity, phylogeny, and chromatin distribution of high mobility group chromosomal proteins 1 and 2. J Biol Chem 260: 9336–9345, 1985

    Google Scholar 

  59. Srebreva L, Zlatanova J, Miloshev G, Tsanev R: Immunological evidence for the existence of H1-like histone in yeast. Eur J Biochem 165: 449–454, 1987

    Google Scholar 

  60. Zlatanova J: Is histone H5 present in mammals? Molec Biol Rep 7: 235–239, 1981

    Google Scholar 

  61. Grimmond HE, Holmes DS: Specific increase of perchloric acid extractable chromosomal proteins during mammalian erythropoiesis. Comp Biochem Physiol 69B: 303–306, 1981

    Google Scholar 

  62. Shimada T, Okihama Y, Murata C, Shukuya R: Occurrence of H1°-like protein and protein A24 in the chromatin of bullfrog erythrocytes lacking histone H5. J Biol Chem 256: 10577–10582, 1981

    Google Scholar 

  63. Yasuda H, Mueller RD, Logan KA, Bradbury EM: Identification of histone H1° in Physarum polycephalum. Its high level in the plasmodial stage increases in amount and phosphorylation in the sclerotial stage. J Biol Chem 261: 2349–2354, 1986

    Google Scholar 

  64. Srebreva L, Zlatanova J: Occurrence of histone H1°-related protein fraction in trout liver. Biochem Biophys Acta 740: 163–168, 1983

    Google Scholar 

  65. Srebreva LN, Andreeva NB, Gasaryan KG, Tsanev RG, Zlatanova JS: Presence of histone H1°-related fraction in chicken liver. Differentiation 25: 113–120, 1983

    Google Scholar 

  66. Miloshev G, Zlatanova J, Srebreva L, Tsanev R: Presence of a H1°-type histone in an invertebrate — the bivalve mollusc Anodonta cygnea. Comp Biochem Physiol 82B: 759–761, 1985

    Google Scholar 

  67. Smith BJ, Cook Y, Johns EW, Weiss RA: Absence of H1° from quiescent chicken cells. FEBS lett 135: 77–80, 1981

    Google Scholar 

  68. Shannon MF, Wigley PL, Wells JRE: Histone H5 and H1° cross-reacting material is restricted to erythroid cells in chicken. FEBS lett 186: 180–186, 1985

    Google Scholar 

  69. Moorman AFM, De Boer PAJ, Smit-Vis JH, Lamers WH, Charles R: Immunological evidence for an H1° type of histone protein in chicken liver. Differentiation 32: 44–48, 1986

    Google Scholar 

  70. Moorman AFM, De Boer PAJ: Immunohistochemical distribution of the histone H1°/H5 variant in various tissues of adult Xenopus laevis. Cell Differentiation 16: 109–117, 1985

    Google Scholar 

  71. Moorman AFM, De Boer PAJ, Linders MTh, Charles R: The histone H5 variant in Xenopus laevis. Cell Differentiation 14: 113–123, 1984

    Google Scholar 

  72. Moorman AFM, De Boer PAJ, Charles R, Lamers WH: The histone H1°/H5 variant and terminal differentiation of cells during development of Xenopus laevis. Differentiation 35: 100–107, 1987

    Google Scholar 

  73. Smith BJ, Harris MR, Sigournay CM, Mayes ELV, Bustin M: A survey of H1°- and H5-like protein structure and distribution in higher and lower eukaryotes. Eur J Biochem 138: 309–317, 1984

    Google Scholar 

  74. Spiker S, Everett KM: Blotting Index of Dissimilarity: use to study immunological relatedness of plant and animal High Mobility Group (HMG) chromosomal proteins. Plant Mol Biol 9: 431–442, 1987

    Google Scholar 

  75. La Bella F, Romani M, Vesco C, Vidali G: High mobility group proteins 1 and 2 are present in simian virus 40 provirions, but not in virions. Nucl Acid Res 9: 121–131, 1981

    Google Scholar 

  76. Mura C, Huang PC, Craig SW: Immunofluorescent study of histone H5 in chick erythroid cells from developing embryos and adults. Mech Ageing Develop 7: 109–122, 1978

    Google Scholar 

  77. Romani M, Vidali G, Tahourdin CSM, Bustin M: Solid phase radioimmunoassay for chromosomal components. J Biol Chem 255: 468–474, 1980

    Google Scholar 

  78. Mitsui Y, Sakagami H: Histone H1 depletion with human cellular aging: biochemical and cytochemical evidences. Acta Histochem Cytochem 16: 403–414, 1983

    Google Scholar 

  79. Bustin M, Stollar BD: Immunochemical specificity in lysine-rich histone subtractions. J Biol Chem 247: 5716–5721, 1972

    Google Scholar 

  80. Sluyser M, Bustin M: Immunological specificities of lysine-rich histones from tumors. J Biel Chem 249: 2507–2511, 1974

    Google Scholar 

  81. Bustin M, Reeder RH, McKnight SL: Immunological cross-reaction between calf and Drosophila histones. J Biol Chem 252: 3099–3101, 1977

    Google Scholar 

  82. Martinez P, Palau J: Antigenic variability among verylysine-rich histones from calf thymus and from sperm of different echinoderms. Comp Biochem Physiol 74B: 611–617, 1983

    Google Scholar 

  83. Pehrson JR, Cohen LH: Embryonal histone HI subtypes of the sea urchin Strongylocentrotus purpuratus: purification, characterization, and immunological comparison with H1 subtypes of the adult. Biochemistry 23: 6761–6764, 1984

    Google Scholar 

  84. Roland B, Pallotta D: An immunological comparison of rye and calf histones. Can J Biochem 56: 1021–1027, 1978

    Google Scholar 

  85. Atanassova RA, Atanassov HL, Koleva ST: Immunechemical investigation of H1 histone variants in pea seedlings. Comp Rend Acad Bulg Sci 39: 109–112, 1986

    Google Scholar 

  86. Atanassova RA: PhD Thesis. Sofia University, 1987

  87. Ivanchenko M, Georgieva E, Ushewa A, Avramova Z: A study on the heterogeneity of histone H1 from dry maize embryos. Eur J Biochem 162: 339–344, 1987

    Google Scholar 

  88. Shay CE, Foster PG, Neelin JM: Predictability of sequence homologies among lysine-rich histones by immunological distance. Comp Biochem Physiol 86B: 193–199, 1987

    Google Scholar 

  89. Michalakis N, Miller OJ, Erlanger BF: Antibodies to histones and histone-histone complexes: immunochemical evidence for secondary structure in histone H1. Science 192: 469–471, 1976

    Google Scholar 

  90. Bustin M, Hopkins RB, Isenberg I: Immunochemical relatedness of high mobility group proteins from calf thymus. J Biol Chem 253: 1694–1699, 1978

    Google Scholar 

  91. Romani M, Rodman TC, Vidali G, Bustin M: Serological analysis of species specificity in the high mobility group chromosomal proteins. J Biol Chem 254: 2918–2922, 1979

    Google Scholar 

  92. Oliver D, Granner D, Chalkley R: Identification of a distinction between cytoplasmic histone synthesis and subsequent histone deposition within the nucleus. Biochemistry 13: 746–749, 1974

    Google Scholar 

  93. Ruiz-Carrillo A, Wangh LJ, Allfrey VG: Processing of newly synthesized histone molecules. Science 190: 117–128, 1975

    Google Scholar 

  94. Jackson V, Shires A, Tanphaichitr N, Chalkley R: Modifications to histones immediately after synthesis. J Mol Biol 104: 471–483, 1976

    Google Scholar 

  95. Tsutsui Y, Suzuki I, Hayashi H: Immunofluorescent study of histone fractions F1 and F2a-1 in cultured cells. Exp Cell Res 94: 63–69, 1975

    Google Scholar 

  96. Kellenberger E, Carlemalm E, Stauffer E, Kellenberger C, Wunderli H: In vitro studies of the fixation of DNA, nucleoprotamine, nucleohistone and proteins. Eur J Cell Biol 25: 1–4, 1981

    Google Scholar 

  97. Wu L, Rechsteiner M, Kuehl L: Comparative studies on microinjected high mobility group chromosomal proteins HMG1 and HMG2. J Cell Biol 91: 488–496, 1981

    Google Scholar 

  98. Bustin M, Neihart NK: Antibodies against chromosomal HMG proteins stain the cytoplasm of mammalian cells. Cell 16: 181–189, 1979

    Google Scholar 

  99. Einck L, Soares N, Bustin M: Localization of HMG chromosomal proteins in the nucleus and cytoplasm by microinjection of functional antibody fragments into living fibroblasts. Exp Cell Res 152: 287–301, 1984

    Google Scholar 

  100. Rechsteiner M, Kuehl L: Microinjection of the nonhistone chromosomal protein HMG1 into bovine fibroblasts and HeLa cells. Cell 16: 901–908, 1979

    Google Scholar 

  101. Einck L, Bustin M: Functional histone antibody fragments traverse the nuclear envelope. J Cell Biol 98: 205–213, 1984

    Google Scholar 

  102. Manser T, Thacher T, Rechsteiner M: Arginine-rich histories do not exchange between human and mouse chromosomes in hybrid cells. Cell 19: 993–1003, 1980

    Google Scholar 

  103. Wu LH, Kuehl L, Rechsteiner M: Dynamic behaviour of historic Hl microinjected into HeLa cells. J Cell Biol 103: 465–474, 1986

    Google Scholar 

  104. Linder S, Zuckerman SH, Ringertz NR: Distribution of histone H5 in chicken erythrocyte-mammalian cell heterokaryons. Exp Cell Res 140: 464–468, 1982

    Google Scholar 

  105. Bergman MG, Wawra E, Winge M: Chicken historic H5 inhibits transcription and replication when introduced into proliferating cells by microinjection. J Cell Sci 91: 201–209, 1988

    Google Scholar 

  106. Mura CV, Stollar BD: Fluorescence-activated sorting of isolated nuclei. Heterogeneity of histone H5 immunofluorescence in chicken erythrocyte nuclei. Exp Cell Res 135: 31–37, 1981

    Google Scholar 

  107. Banchev T, Srebreva L, Zlatanova J, Tsanev R: Immunofluorescent localization of histone H1° in the nuclei of proliferating and differentiating Friend cells. Exp Cell Res 177: 1–8, 1988

    Google Scholar 

  108. Hutchison N, Weintraub H: Localization of DNase Isensitive sequences to specific regions of interphase nuclei. Cell 43: 471–482, 1985

    Google Scholar 

  109. Bustin M: Immunological approaches to chromatin and chromosome structure and function. In: W Arber et al. (eds) Current Topics in Microbiology and Immunology. Springer-Verlag, Berlin, Heidelberg, New York, 1979, vol 88, pp 105–142

    Google Scholar 

  110. Kurth PD, Moudrianakis EN, Bustin M: Historic localization in polytene chromosomes by immunofluorescence. J Cell Biol 78: 910–918, 1978

    Google Scholar 

  111. Eisen H, Gjerset R, Hasthorpe S: Distribution and regulation of historic H1° in rodents. In: CW Lloyd, DA Rees (eds) Cellular controls in differentiation. Academic Press, London, New York, Toronto, Sidney, San Francisco, 1981, pp 215–230

    Google Scholar 

  112. Alfageme CR, Rudkin GT, Cohen LH: Localization of chromosomal proteins in polytene chromosomes. Proc Natl Acad Sci USA 73: 2038–2042, 1976

    Google Scholar 

  113. Jamrich M, Greenleaf AL, Bautz EKF: Localization of RNA polymerase in polytene chromosomes of Drosophila melanogaster. Proc Natl Acad Sci USA 74: 2079–2083, 1977

    Google Scholar 

  114. Jamrich M, Greenleaf AL, Bautz FA, Bautz EK: Functional organization of polytene chromosomes. Cold Spring Harbor Symp Quant Biol: XLII 389–396, 1978

    Google Scholar 

  115. Greenleaf AL, Plagens U, Jamrich M, Bautz EKF: RNA polymerase B in heat induced puffs of Drosophila polytene chromosomes. Chromosoma 65: 127–136, 1978

    Google Scholar 

  116. Kurth PD, Bustin M: Localization of chromosomal protein HMG-1 in polytene chromosomes of Chronomus thummi. J Cell Biol 89: 70–77, 1981

    Google Scholar 

  117. Kurth PD, Reisch JC, Bustin M: Selective exposure of antigenic determinants in chromosomal proteins upon gene activation in polytene chromosomes. Exp Cell Res 143: 257–269, 1983

    Google Scholar 

  118. Zick Y, Goldblatt D, Bustin M: Exposure of histone F, subfractions in chromatin. Biochem Biophys Res Commun 65: 637–643, 1975

    Google Scholar 

  119. Bustin M, Kupfer H: Purification of anti-histone-H1 antibodies and their use in measuring histone determinants in chromatin by radioimmunoadsorbance. Biochem Biophys Res Commun 68: 718–723, 1976

    Google Scholar 

  120. Takahashi K, Tashiro Y: Binding of antibodies against historic Hl to unfolded and folded nucleofilaments. Eur J Biochem 97: 353–360, 1979

    Google Scholar 

  121. Di Padua Mathieu D, Mura CV, Frado L-LY, Woodcock CLF, Stollar BD: Differing accessibility in chromatin of the antigenic sites of regions 1–58 and 63–125 of histone H2B. J Cell Biol 91: 135–141, 1981

    Google Scholar 

  122. Mendelson E, Landsman D, Druckmann S, Bustin M: Immunofractionation of chromatin regions associated with historic H1°. Eur J Biochem 160: 253–260, 1986

    Google Scholar 

  123. Bustin M, Feldmann RJ: Interactive computer surface graphic study of the binding of an antibody to the chromatin subunit. J Theor Biol 92: 97–102, 1981

    Google Scholar 

  124. Pospelov VA, Jerkin AM, Khachatrian AT: H1 and H5 historic arrangement in chromatin of pigeon erythrocytes. FEBS lett 128: 315–317, 1981

    Google Scholar 

  125. Torres-Martinez S, Ruiz-Carrillo A: Nucleosomes containing histories H1 or H5 are closely interspersed in chromatin. Nucl Acid Res 10: 2323–2335, 1982

    Google Scholar 

  126. Mendelson E, Bustin M: Monoclonal antibodies against distinct determinants of histne H5 bind to chromatin. Biochemistry 23: 3459–3466, 1984

    Google Scholar 

  127. Dimitrov SI, Russanova VR, Pashev IG: The globular domain of historic H5 is internally located in the 30 rim chromatin fiber: an immunochemical study. EMBO J 6: 2387–2392, 1987

    Google Scholar 

  128. Shick VV, Belyavsky AV, Mirzabekov AD: Primary organization of nucleosomes. Interaction of non-histone high mobility group proteins 14 and 17 with nucleosomes, as revealed by DNA-protein cross-linking and immunoaffinity isolation. J Mol Biol 185: 329–339, 1985

    Google Scholar 

  129. Tahourdin CSM, Neihart NK, Isenberg I, Bustin M: Immunochemical detection of chromosomal protein HMG-17 in chromatin subunits. Biochemistry 20: 910–915, 1981

    Google Scholar 

  130. Malik N, Smulson M, Bustin M: Enrichment of acetylated histones in polynucleosomes containing high mobility group protein 17 revealed by immunoaffinity chromatography. J Biol Chem 259: 699–702, 1984

    Google Scholar 

  131. Mathis D, Oudet P, Chambon P: Structure of transcribing chromatin. Progr Nucl Acids Res Mol Biol 24: 1–55, 1980

    Google Scholar 

  132. Delabar JM: Nonrandom location of H1-H1° histones of chromatin of mouse liver and brain. J Biol Chem 260: 12622–12628, 1985

    Google Scholar 

  133. Roche T, Gorka C, Goeltz P, Lawrence JJ: Association of histone H1° with a gene repressed during liver development. Nature 314: 197–198, 1985

    Google Scholar 

  134. Dorbic T, Wittig B: Isolation of oligonucleosomes from active chromatin using HMG-17-specific monoclonal antibodies. Nucl Acid Res 14: 3363–3376, 1986

    Google Scholar 

  135. Druckmann S, Mendelson E, Landsman D, Bustin M: Immunofractionation of DNA sequences associated with HMG-17 in chromatin. Exp Cell Res 166: 486–496, 1986

    Google Scholar 

  136. McKnight SL, Bustin M,Miller OL: Electron microscopic analysis of chromosome metabolism in the Drosophila melanogaster embryo. Cold Spring Harbor Symp Quant Biol XLII: 741–754, 1978

    Google Scholar 

  137. Sperling R, Scheer U, Goldblatt D, Bustin M: Histories are associated with ribosomal DNA during transcription. An immunoelectron microscopic study. Cited in ref. 105

  138. Eisen H, Hasthorpe S, Gjerset R, Nasi S, Keppel F: Distribution and behaviour of the chromosomal protein IP25 in vivo and tissue culture. In: GB Rossi (ed) In vivo and in vitro erythropoiesis: the Friend system. Elsevier/ North Holland Biomedical Press, Amsterdam, New York, Oxford, 1980, pp 289–296

    Google Scholar 

  139. Gjerset R, Gorka C, Hasthorpe S, Lawrence JJ, Eisen H: Developmental and hormonal regulation of protein H1° in rodents. Proc Natl Acad Sci USA 79: 2333–2337, 1982

    Google Scholar 

  140. Kleinschmidt JA, Scheer U, Dabauvalle MC, Bustin M, Franke WW: High mobility group proteins of amphibian oocytes: a large storage pool of a soluble high mobility group-l-like protein and involvement in transcriptional events. J Cell Biol 97: 838–848, 1983

    Google Scholar 

  141. Scheer U, Sommerville J, Bustin M: Injected histone antibodies interfere with transcription of lampbrush chromosome loops in oocytes of Pleurodeles. J Cell Sci 40: 1–20, 1979

    Google Scholar 

  142. Einck L, Bustin M: Inhibition of transcription in somatic cells by microinjection of antibodies to chromosomal proteins. Proc Natl Acad Sci USA 80: 6735–6739, 1983

    Google Scholar 

  143. Mura CV, Craig CW, Huang PC: Immunofluorescent and autoradiographic analysis of the relationship between the presence of histone H5 and DNA synthesis in developing chick erythroid cells. Exp Cell Res 114: 301–305, 1978

    Google Scholar 

  144. Alexandrova EA, Marekov LN, Beltchev BG: Involvement of protein HMG1 in DNA replication. FEBS lett 178: 153–156, 1984

    Google Scholar 

  145. McKnight SL, Miller OL: Electron microscopic analysis of chromatin replication in the cellular blastoderm of Drosophila melanogaster embryo. Cell 8: 795–827, 1977

    Google Scholar 

  146. Bavykin SG, Studitsky VM, Natcheva GA, Mirzabekov AD, Srebreva L, Banchev T, Zlatanova J, Tsanev R: New immunochemical method of detection of proteins inter acting with DNA during replication. Int Symp Phys Chem of DNA and Molecular Mechanisms of Genome Functioning. Tbilisi 6–8 August 1987, Abstracts p 40

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  1. Molecular Genetics Lab, Institute of Genetics, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    Jordanka S. Zlatanova

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Zlatanova, J.S. Immunochemical approaches to the study of histone H1 and high mobility group chromatin proteins. Mol Cell Biochem 92, 1–22 (1990). https://doi.org/10.1007/BF00220715

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  • DOI: https://doi.org/10.1007/BF00220715

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