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Observations on the cytolemma of the olfactory receptor cell in the newt

1. Freeze replica analysis

Summary

The fine structure of the cytolemma of olfactory receptor cells in the newt was studied by the freeze-fracture replica method. Two kinds of receptor cells were recognized, namely ciliated cells (ciliary type) and non-ciliated cells (microvilli type). The cytolemma of olfactory knobs as well as their processes from both types of receptor cells showed an abundance of large membrane particles 80∼110Å in diameter. The large square aggregation of membrane particles, 0.1×0.1 μm to 0.2×0.3 μm in size, consisting of 50∼100 cuboidal subunits, were found in the cytolemma of the dendrite. A structural model of aggregation is presented. The soma of the receptor cell revealed large pitted membrane particles about 140Å in diameter. These particles are possibly the morphologic counterpart to ionophores which have been proposed by electrophysiological studies.

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Correspondence to Jiro Usukura.

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Usukura, J., Yamada, E. Observations on the cytolemma of the olfactory receptor cell in the newt. Cell Tissue Res. 188, 83–98 (1978). https://doi.org/10.1007/BF00220516

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Key words

  • Olfactory epithelium (Newt)
  • Receptor membrane
  • Membrane particles
  • Square aggregation
  • Freeze-replica