Skip to main content
SpringerLink
Log in

The nucleolar fibrillar centres in various cell types in vivo or in vitro

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

Nucleoli were studied in chick fibroblasts cultured in vitro, under normal or under experimental conditions, and in several mammalian cell types in vivo. All these cells frequently contain nucleoli with fibrillar centres. The nucleolar fibrillar centres are composed of fibrous material of low electron density and are always intimately associated with the dense fibrillar component. Their morphology is very similar to that analysed cytochemically in Ehrlich tumour cells. It therefore appears that they could be related to the nucleolar organizers as suggested in Ehrlich tumour cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anteunis, A., Pouchelet, M., Gansmuller, A., Robineaux, R.: Mise en évidence ultrastructurale d'éléments sensibles à l'action de la désoxyribonucléase dans les zones fibrillaires des nucléoles des noyaux interphasiques des cellules L 929. C.R. Acad. Sci. (Paris) 281, 901–903 (1975)

    Google Scholar 

  • Anteunis, A., Pouchelet, M., Robineaux, R., Vial, M.: Ultrastructure des acides nucléiques et en particulier de l'ADN nucléolaire des cellules L 929 après coloration par la 3–3′ diaminobenzidine (DAB) oxydée. C.R. Acad. Sci. (Paris) 277, 1169–1171 (1973)

    Google Scholar 

  • Chouinard, L.A.: Localization of intranucleolar DNA in root meristematic cells of Allium cepa. J. Cell Sci. 6, 73–85 (1970)

    Google Scholar 

  • Chouinard, L.A.: Behavior of the structural components of the nucleolus during mitosis in Allium cepa. In: Advances in cytopharmacology, Vol. I, pp. 69–87 (F. Clementi and B. Ceccarelli, eds.). New York: Raven Press 1971

    Google Scholar 

  • Chouinard, L.A.: An electron-microscope study of the intranucleolar chromatin in root meristematic cells of Allium cepa. J. Cell Sci. 15, 645–657 (1974)

    Google Scholar 

  • Chouinard, L.A.: An electron-microscope study of the intranucleolar chromatin during nucleologenesis in root meristematic cells of Allium cepa. J. Cell Sci. 19, 85–102 (1975)

    Google Scholar 

  • Dutta, C.R., Siegesmund, K.A., Fox, C.A.: Light and electron microscopic observations of an intranucleolar body in nerve cells. J. Ultrastruct. Res. 8, 542–551 (1963)

    Google Scholar 

  • Goessens, G.: Les “centres fibrillaires” des nucléoles de cellules tumorales d'Ehrlich. C.R. Acad. Sci. (Paris) 277, 325–327 (1973)

    Google Scholar 

  • Goessens, G.: Stereological analysis of Ehrlich tumor cell nucleoli. J. Microscopie 19, 12a (1974a)

  • Goessens, G.: Etude autoradiographique au microscope électronique des nucléoles de cellules tumorales d'Ehrlich. Mise en évidence d'ADN au niveau des centres fibrillaires. C.R. Acad. Sci. (Paris) 279, 991–993 (1974b)

    Google Scholar 

  • Goessens, G.: High resolution autoradiographic studies of Ehrlich tumour cell nucleoli. Nucleolar labelling after 3H-actinomycin D binding to DNA or after 3H-thymidine or 3H-uridine incorporation in nucleic acids. Exp. Cell Res. 100, 88–94 (1976)

    Google Scholar 

  • Goessens, G., Lepoint, A.: The fine structure of the nucleolus during interphase and mitosis in Ehrlich tumour cells cultivated in vitro. Exp. Cell Res. 87, 63–72 (1974)

    Google Scholar 

  • Granboulan, N., Granboulan, Ph.: Cytochimie ultrastructurale du nucléole. II. Etude des sites de synthèse du RNA dans le nucléole et le noyau. Exp. Cell Res. 38, 604–619 (1965)

    Google Scholar 

  • Granick, S., Granick, D.: Nucleolar necklaces in chick embryo myoblasts formed by lack of arginine. J. Cell Biol. 51, 636–642 (1971)

    Google Scholar 

  • Hubert, J.: Données préliminaires sur les “centres fibrillaires” du nucléole de certaines cellules du follicule ovarien d'un Lézard: Lacerta muralis Laur. C.R. Acad. Sci. (Paris) 281, 271–273 (1975)

    Google Scholar 

  • Lafontaine, J.G., Lord, A.: An ultrastructural and radioautographic investigation of nucleolonemal component of plant interphase nucleoli. J. Cell Sci. 12, 369–383 (1973)

    Google Scholar 

  • Lafontaine, J.G., Lord, A.: A correlated light-and electronmicroscope investigation of the structural evolution of the nucleolus during the cell cycle in plant meristematic cells (Allium porrum). J. Cell Sci. 16, 63–93 (1974)

    Google Scholar 

  • Lord, A., Lafontaine, J.G.: The organization of the nucleolus in meristematic plant cells. A cytochemical study. J. Cell Biol. 40, 633–647 (1969)

    Google Scholar 

  • Paermentier, F. de, Bassleer, R., Lepoint, A., Desaive, Cl., Goessens, G., Lhoest-Gautier, M.P.: Mode of action of amphotericin B on chick embryo fibroblasts and on mouse Ehrlich tumour cells: a cytological and cytochemical analysis. J. Cell Sci. 18, 441–454 (1975)

    Google Scholar 

  • Paermentier, F. de, Bassleer, R.: Effets de la cordycepine sur des fibroblasts de poulet cultivés in vitro. Bull. Ass. Anat. (Nancy) 59, 153–156 (1975)

    Google Scholar 

  • Penman, S., Rosbach, M., Penman, M.: Messenger and heterogeneous nuclear RNA in HeLa cells: differential inhibition by cordycepin. Proc. nat. Acad. Sci. (Wash.) 67, 1878–1885 (1970)

    Google Scholar 

  • Phillips, S.G., Phillips, D.M.: Nucleoli of diploid cell strains. Their normal ultrastructure and the effects of toyocamycin and actinomycin D. J. Cell Biol. 49, 785–802 (1971)

    Google Scholar 

  • Pouchelet, M., Gansmuller, A., Anteunis, A., Robineaux, R.: Mise en évidence en microscopie électronique, dans des noyaux interphasiques des cellules L 929, de filaments de DNA associés aux zones fibrillaires RNA des nucléoles. C.R. Acad. Sci. (Paris) 280, 2461–2463 (1975)

    Google Scholar 

  • Recher, L., Parry, N., Whitescarver, J., Briggs, L.: Lead-positive nuclear structures and their behavior under the effect of various drugs. J. Ultrastruct. Res. 38, 398–410 (1972)

    Google Scholar 

  • Recher, L., Whitescarver, J., Briggs, L.: The fine structure of a nucleolar constituent. J. Ultrastruct. Res. 29, 1–14 (1969)

    Google Scholar 

  • Recher, L., Whitescarver, J., Briggs, L.: A cytochemical and radioautographic study of human tissue culture cell nucleoli. J. Cell Biol. 45, 479–492 (1970)

    Google Scholar 

  • Schoefl, G.I.: The effect of actinomycin D on the fine structure of the nucleolus. J. Ultrastruct. Res. 10, 224–243 (1964)

    Google Scholar 

  • Simard, R.: Specific nuclear and nucleolar ultrastructural lesions induced by proflavin and similar acting antimetabolites in tissue culture. Cancer Res. 26, 2316–2328 (1966)

    Google Scholar 

  • Simard, R., Bernhard, W.: Le phénomène de la ségrégation nucléolaire: spécificité d'action de certains antimétabolites. Int. J. Cancer 1, 463–479 (1966)

    Google Scholar 

  • Smetana, K., Gyorkey, F., Gyorkey, Ph., Busch, H.: Compact filamentous bodies of nuclei and nucleoli of human prostate gland. Exp. Cell Res. 64, 133–139 (1971)

    Google Scholar 

  • Studzinski, G.P., Gierthy, J.F., Cytologic appearance of the nucleolus of normal and neoplastic cells in relation to the synthesis of RNA. Acta cytol. (Philad.) 16, 245–248 (1972)

    Google Scholar 

  • Yasuzumi, G., Sugihara, R.: The fine structure of nuclei as revealed by electron microscopy. The fine structure of Ehrlich Ascites tumor cell nuclei in preprophase. Exp. Cell Res. 37, 207–229 (1965)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut d'Histologie, Université de Liège, Liège, Belgique

    G. Goessens

Authors
  1. G. Goessens
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Goessens, G. The nucleolar fibrillar centres in various cell types in vivo or in vitro. Cell Tissue Res. 173, 315–324 (1976). https://doi.org/10.1007/BF00220319

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00220319

Key words

Search

Navigation