Summary
Following castration, the nucleus of the dark cells of the subcortical portions of the collecting tubules in the rat kidney showed two types of morphological alterations: 1) Decomposition of the nuclear membrane in a circumscribed area. This phenomenon is independent of the sex of the animal and becomes more frequent up to the 30th day after castration. Occasionally, decomposed nuclei can also be observed in controls. 2) Giant nuclear bodies. These inclusions occurred only in castrated males. Early stages were already observed at the 10th day after orchiectomy, whereas fully developed giant nuclear bodies could not be demonstrated until about the 20th day after castration.
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References
Bargmann W (1978) Das Sammelrohrsystem. In: Handbuch der mikroskopischen Anatomie des Menschen. Band 7. Harn- und Geschlechtsapparat. Teil 5. Springer-Verlag, Berlin, pp 205–215
Bloom GD (1967) A nucleus with cytoplasmatic features. J Cell Biol 35:266–268
Brandes D, Schofield B, Anton E (1965) Nuclear mitochondriae. Science 149:1373–1374
Bucciarelli E (1966) Intranuclear cisternae resembling structures of the Golgi complex. J Cell Biol 30:664–665
Costa CB, Paula-Barbosa MM (1979) Nuclear inclusions in the nerve cells of the sphenopalatine ganglion of the dog. Cell Tissue Res 201:289–294
Deimling Ov (1970) Enzymarchitektur der Niere und Sexualhormone. Progr Histochem 1:1–50
Dupuy-Coin AM, Bouteille M (1972) Developmental pathway of granular and beaded nuclear bodies from nucleoli. J Ultrastruct Res 40:55–67
Dupuy-Coin AM, Kalifat SR, Bouteille M (1972) Nuclear bodies as proteinaceous structures containing ribonucleoproteins. J Ultrastruct Res 38:174–187
Hagege J, Riebet G (1975) Dark cells of the distal convoluted tubules and collecting ducts. I. Morphological data. Fortschr Zool 23:289–298
Heidenhain R (1947) Mikroskopische Beiträge zur Anatomie und Physiologie der Nieren. Arch Mikroskop Anat Entwicklungsmech 10:1–50
Lawson RH, Ali S (1975) Ultrastructure of white-streaked Dendrobium phalaenopsis orchid flowers infected with a rhabdovirus. J Ultrastruct Res 53:345–353
Luciano L (1967) Die Feinstruktur der Tränendrüse der Ratte und ihr Geschlechtsdimorphismus. Z Zellforsch 76:1–20
Müller HA, Ramin Dv (1963) Morphologie und Morphogenese der durch Schwermetalle (Pb, Bi) hervorgerufenen Kerneinschlüsse in den Hauptstückepithelien der Rattenniere. Beitr Pathol Anat 128:445–467
Ortmann R (1955) Histochemische Untersuchungen an menschlicher Placenta mit besonderer Berücksichtigung der Kernkugeln (Kerneinschlüsse) und der Plasmalipoideinschlüsse. Z Anat 119:28–54
Ortmann R (1956) Histochemische Untersuchungen an den Kerneinschlußkörpern der Ganglienzellen im Nucleus praeopticus des Karpfens (Cyprinus carpio (Linne)). Z Anat 119:485–499
Richet G, Hagege J (1975) Dark cells of the distal convoluted tubules and collecting ducts. II. Physiological significance. Fortschr Zool 23:299–306
Robertson DM (1964) Electron microscopic studies of nuclear inclusions in meningiomas. Am J Pathol 45:835–847
Schachowa S (1876) Untersuchungen über die Nieren. Dissertation, Bern
Schiebler TH, Danner KG (1978) The effect of sex hormones on the proximal tubules in the rat kidney. Cell Tissue Res 192:527–549
Summers MD (1971) Electron microscopic observations on granulosis virus entry, uncoating and replication processes during infection of the midgut cells of Trichoplusia ni. J Ultrastruct Res 35:606–625
Tanada Y, Leutenegger R (1970) Multiplication of a granulosis virus in larval midgut cells of Trichoplusia ni and possible pathways of invasion into the hemocoel. J Ultrastruct Res 30:589–600
Warchol JB (1978) Intranuclear microfilament bundles in the ependymal cells of the third ventricle of the rat. Cell Tissue Res 194:353–359
Weber A, Whipp S, Usenik E, Frommes S (1964) Structural changes in the nuclear body in the adrenal zona fasciculata of calf following the administration of ACTH. J Ultrastruct Res 11:564–576
Zabel M, Schiebler TH (1980) Histochemical, autoradiographic and electron microscopic investigations of the renal proximal tubule of male and female rats after castration. Histochemistry (in press)
Zeller J (1973) Zur Cytochemie der Lysosomen in der Rattenniere unter normalen und experimentellen Bedingungen. Histochemie 35:235–262
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Supported by the Deutsche Forschungsgemeinschaft (SFB 105)
Research fellow of the Alexander von Humboldt Foundation
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Zabel, M. Alterations in nuclear structure in dark cells of the collecting tubules in the kidney of castrated rats. Cell Tissue Res. 210, 485–492 (1980). https://doi.org/10.1007/BF00220204
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DOI: https://doi.org/10.1007/BF00220204