Skip to main content
Log in

Pancreatic islet cytology of ictaluridae (Teleostei)

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

The endocrine pancreas of the bullhead catfish, Ictalurus nebulosus, and the channel catfish, I. punctatas was studied by light and electron microscopy. In addition to the usual A, B and D cells, a fourth endocrine cell type was consistently observed in the electron microscope. All endocrine cell types were innervated. The vesicles of most of the nerve endings were ultrastructurally different from typical adrenergic and cholinergic vesicles, strongly suggesting the possibility of a third autonomic neurotransmitter serving as a regulator of catfish islet secretion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bencosme, S. A., Meyer, J., Bergman, B. J., Martinez-Palamo, A.: The principal islet of the bullhead fish (Ictalurus nebulosus). Rev. Can. Biol. 24, 141–154 (1965)

    Google Scholar 

  • Brinn, J.: The pancreatic islets of bony fishes. Amer. Zool. 13, 653–665 (1973)

    Google Scholar 

  • Brinn, J., Epple, A.: Structure and ultrastructure of the specialized islet organ of the American eel, Anguilla rostrata. Anat. Rec. 172, 277 (1972)

    Google Scholar 

  • Bohman, S. O., Maunsbach, A. B.: Effects on tissue fine structure of variations in colloid osmotic pressure of glutaraldehyde fixatives. J. Ultrastruct. Res. 30, 195–208 (1970)

    Google Scholar 

  • Boquist, L., Patent, G.: The pancreatic islets of the teleost Scorpaena scropha. An ultrastructural study with particular regard to fibrillar granules. Z. Zellforsch. 115, 416–425 (1971)

    Google Scholar 

  • Burnstock, G.: Purinergic nerves. Pharmacol. Rev. 24, 509–581 (1972)

    Google Scholar 

  • Bussolati, G., Capella, C., Vasallo, G., Solcia, E.: Histochemical and ultrastructural studies on pancreatic A cells. Evidence for glucagon and non-glucagon components of the α granule. Diabetologia 7, 181–188 (1971)

    Google Scholar 

  • Cavallero, C., Spagnoli, L. G., Cavallero, M.: Ultrastructural study of the human pancreatic islets. Arch. Histol. Jap. 36, 307–321 (1974)

    Google Scholar 

  • Chidambaram, S., Meyer, R. K., Hasler, A. D.: Effect of hypophysectomy, isletectomy and ACTH on glycemia and hematocrit in the bullhead, Ictalurus melas. J. exp. Zool. 184, 75–80 (1973)

    Google Scholar 

  • Creutzfeldt, W., Arnold, R., Creutzfeldt, C.: Gastrin and G-cells in the antral mucosa of patients with pernicious anemia, acromegaly and hyperthyroidism and in a Zollinger-Ellison tumor of the pancreas. Europ. J. clin. Invest. 1, 461–479 (1971)

    Google Scholar 

  • Deconinck, J. F., Assch, F. A. van, Potvliege, P. R., Gepts, W.: The ultrastructure of the human pancreatic islets II. The islets of neonates. Diabetologia 8, 326–333 (1972)

    Google Scholar 

  • Deconinck, J. F., Potvliege, P. R., Gepts, W.: The ultrastructure of the human pancreatic islets I. The islets of adults. Diabetologia 7, 266–282 (1971)

    Google Scholar 

  • Epple, A.: A staining sequence for A, B and D cells of pancreatic islets. Stain Technol. 42, 53–61 (1967)

    Google Scholar 

  • Epple, A.: The endocrine pancreas. In: Fish Physiology. Vol. II (W. S. Hoar and D. J. Randall, eds.), p. 275–319 New York: Academic Press 1969

    Google Scholar 

  • Falkmer, S., Olsson, R.: Ultrastructure of the pancreatic islet tissue of normal and alloxantreated Cottus scorpius. Acta endocr. (Kbh.) 39, 32–46 (1962)

    Google Scholar 

  • Falkmer, S., Patent, G.: Comparative and embryological aspects of the pancreatic islets. In: Handbook of Physiology, Sect. 7, vol. I. The endocrine pancreas (D. F. Steiner and N. Freinkel, eds.), p. 1–24. Baltimore: Williams and Wilkins, Co. 1972

    Google Scholar 

  • Gomori, G.: Observations with differential stains of human islets of Langerhans. Amer. J. Path. 17, 395–406 (1941)

    Google Scholar 

  • Greider, M., McGuigan, J.: Cellular localization of gastrin in human pancreas. Diabetes 20, 389–396 (1971)

    Google Scholar 

  • Grossner, D.: Licht- und elektronenmikroskopische Untersuchungen des Inselorgans der Aale (Conger conger, Muraena helena, Anguilla anguilla). Anat. Anz. 125, 177–184 (1969)

    Google Scholar 

  • Hellman, B., Hellerström, C.: The islets of Langerhans in ducks and chickens with special reference to the argyrophil reaction. Z. Zellforsch. 52, 278–290 (1960)

    Google Scholar 

  • Jirasek, K., Kubes, L.: A new endocrine cell type of the human pancreatic islets. Experientia (Basel) 28, 966–967 (1972)

    Google Scholar 

  • Karnovsky, M.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137A (1965)

  • Klein, C.: Innervation des cellules du pancreas du Poisson téléostéen Xiphophorus helleri H. Z. Zellforsch. 113, 564–580 (1971)

    Google Scholar 

  • Klein, C., Lange, R.: Mise en évidence par immunofluorescence des cellules sécrétrices de glucagon dans le pancréas endocrine du Poisson téléostéen Xiphophorus helleri H. Histochemie 29, 213–219 (1972)

    Google Scholar 

  • Klein, C., Lange, R. H.: Mise en évidence, par comparison de coupes semifines, traitées par immunofluorescence, et de coupes ultra-fines adjacentes, de deux types de cellules sécrétrices de glucagon dans le pancréas endocrine du poisson téléostéen Xiphophorus helleri H. C. R. Acad. Sci. (Paris) 278, 1609–1612 (1974a)

    Google Scholar 

  • Klein, C., Lange, R. H.: Mise en évidence, par comparison de coupes semifines et ultra-fines successives, des types cullulaires autres que les cellules à glucagon dans le pancréas endocrine du poisson téléostéen Xiphophorus helleri H. C. R. Acad. Sci. (Paris) 278, 1747–1749 (1974b)

    Google Scholar 

  • Kobayashi, K., Takahashi, Y.: Light and electron microscopic observations on the islets of Langerhans in Carassius carassius longsdorfii. Arch. Histol. Jap. 31, 433–454 (1970)

    Google Scholar 

  • Kobayashi, D., Takahashi, Y.: Fine structure of Langerhans' islet cells in a marine teleost Conger japonicus Blocker. Gen. Comp. Endocr. 23, 1–18 (1974)

    Google Scholar 

  • Kudo, S., Takahashi, Y.: New cell typus of the pancreatic islets in the crucian carp, Carassius carassius. Z. Zellforsch. 146, 425–438 (1973)

    Google Scholar 

  • Lacy, P. E., Greider, M. H.: Ultrastructural organization of mammalian pancreatic islets. In: Handbook of Physiology, Sect. 7, vol. I. The endocrine pancreas (D. F. Steiner and and N. Freinkel, eds.), p. 77–89. Baltimore: Williams and Wilkins, Co. 1972

    Google Scholar 

  • Lewis, T., Epple, A.: Pancreatectomy in the eel: effects on serum glucose and cholesterol. Science 178, 1286–1288 (1972)

    Google Scholar 

  • Lomsky, R., Langr, F., Vortel, V.: Immunohistochemical demonstration of gastrin in mammalian islets of Langerhans. Nature (Lond.) 223, 618–619 (1969)

    Google Scholar 

  • Lotstra, F., van der Loo, W., Gepts, W.: Are gastrin-cells present in mammalian pancreatic islets ? Diabetologia 10, 291–302 (1974)

    Google Scholar 

  • Minick, M., Chavin, W.: Effects of vertebrate insulins upon serum FFA and phospholipid levels in the goldfish, Carassius auratus Linnaeus. Comp. Biochem. Physiol. 41A, 791–804 (1972a)

    Google Scholar 

  • Minick, M., Chavin, W.: Effects of alloxan, streptozotocin or D-mannoheptulose upon serum free fatty acid and serum glucose levels in goldfish, Carassius auratus L. Comp. Biochem. Physiol. 41B, 367–376 (1972b)

    Google Scholar 

  • Munger, B. L.: The biology of secretory tumors of the pancreatic islets. In: Handbook of Physiology, Sect. 7, vol. I. The endocrine pancreas (D. F. Steiner and N. Freinkel, eds.), p. 305–314. Baltimore: Williams and Wilkins, Co. 1972

    Google Scholar 

  • Munger, B. L., Caramia, F., Lacy, P. E.: The ultrastructural basis for the identification of cell types in the pancreatic islets II. Babbit, dog, and opossum. Z. Zellforsch. 67, 776–798 (1965)

    Google Scholar 

  • Nakamura, M., Yamada, K., Yokote, M.: Ultrastructural aspects of the pancreatic islets of spontaneous diabetes mellitus. Experientia (Basel) 27, 75–76 (1971)

    Google Scholar 

  • Nakamura, M., Yokote, M.: Ultrastructural studies on the islets of Langerhans of the carp. Z. Anat. Entwickl.-Gesch. 134, 61–72 (1971)

    Google Scholar 

  • Rhoten, W. B.: The cell population in the pancreatic islets of Amphisbaenidae — A light and electron microscopic study. Anat. Rec. 167, 401–424 (1970)

    Google Scholar 

  • Smith, P. H.: Pancreatic islets of the coturnix quail. A light and electron microscopic study with special reference to the islet organ of the splenic lobe. Anat. Rec. 178, 567–586 (1974)

    Google Scholar 

  • Thomas, N.: Morphology of the endocrine cells in the islet tissue of the cod Gadus callarias. Acta endocr. (Kbh.) 63, 679–695 (1970)

    Google Scholar 

  • Tobe, T., Henmi, K., Pukuchi, K., Chen, S., Tachibana, S.: Distribution of gastrin in human digestive organs demonstrated by direct immunofluorescence. Arch. Histol. Jap. 37, 59–72 (1974)

    Google Scholar 

  • Watari, N., Tsukagoshi, N., Honma, Y.: The correlative light and electron microscopy of the islets of Langerhans of some lower vertebrates. Arch. Histol. Jap. 31, 371–392 (1970)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Supported in part by PHS grant AM 11407 awarded to Dr. Bryce Munger.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brinn, J.E. Pancreatic islet cytology of ictaluridae (Teleostei). Cell Tissue Res. 162, 357–365 (1975). https://doi.org/10.1007/BF00220182

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00220182

Key words

Navigation