Summary
Following the K-antimonate reaction in atrial myocardial tissue, a pattern of evenly spaced cross striations of antimonate precipitates is demonstrated along the myofilaments. This spacing, found in both turtle and mouse atria, has a periodicity of about 400 Å. In order to test the shifts of the antimonate reaction product in the tissue, a comparison is made between the localization of the antimonate precipitate as seen in viz. thin plastic sections and in cryo-ultra sections being dry-cut at -90° C from N2 frozen tissue. Preliminary results suggest only minor distributional differences in the sarcomeric pattern. On the basis of these tests, and, on the basis of previous studies by means of X-ray microanalysis, it is suggested that the periodic pattern of evenly spaced precipitates, reflects the localization of troponin bound calcium along the thin filaments during contraction.
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Christensen, A. K.: Frozen thin sections of fresh tissue for electron microscopy, with a description of pancreas and liver. J. Cell Biol. 51, 772–804 (1971)
Clemente, F., Meldolesi, J.: Calcium and pancreatic secretion. I. Subcellular distribution of calcium and magnesium in the exocrine pancreas of the Guinea pig. J. Cell Biol. 65, 88–102 (1975)
Ebashi, S., Ebashi, F., Kodama, A.: Troponin as the Ca++-receptive protein in the contractile system. J. Biochem. (Tokyo) 62, 137–138 (1967)
Ebashi, S., Endo, M.: Calcium ion and muscle contraction. In: Progr. Biophys. molec. Biol. (eds. Butler, J. A. V., Noble, D.), p. 125–183, Oxford: Pergamon Press 1968
Hartshorne, D. J.: Interactions of desensitized actomyosin with tropomyosin, troponin A, troponin B, and polyanions. J. gen. Physiol. 55, 585–601 (1970)
Huxley, H. E., Brown, W.: The low-angle X-ray diagram of vertebrate striated muscle and its behaviour during contraction and rigor. J. molec. Biol. 30, 383–434 (1967)
Katz, A. M., Goodhart, P. J., Goodhart, H. L.: Calcium and the cardiac contractile proteins. In: Calcium and the heart (eds. Harris, P., Opie, L.), p. 124–134. London and New York: Academic Press 1971
Klein, R. L., Yen, S. S., Thureson-Klein, A.: Critique on the K-pyroantimonate method for semiquantitative estimation of cations in conjugation with electron microscopy. J. Histochem. Cytochem. 20, 65–78 (1972)
Komnick, H.: Elektronenmikroskopische Lokalisation von Na+ und Cl- in Zellen und Geweben. Protoplasma (Wien) 55, 414–418 (1962)
Langer, G. A.: Ion fluxes in cardiac excitation and contraction and their relation to myocardial contractility. Physiol. Rev. 48, 708–757 (1968)
Legato, M. J., Langer, G. A.: The subcellular localization of calcium ion in mammalian myocardium. J. Cell Biol. 41, 401–423 (1969)
Maccallister, L. P., Hadek, R.: The localization of calcium in skeletal muscle: Its distribution in muscle in which the caffeine-induced contracture was arrested. J. Ultrastruct. Res. 45, 59–81 (1973)
Ohtsuki, I., Masaki, T., Nonomura, Y., Ebashi, S.: Periodic distribution of troponin along the thin filament. J. Biochem. (Tokyo) 61, 817–819 (1967)
Olson, R. E.: Excitation-contraction coupling. In: Calcium and the heart (eds. Harris, P., Opie, L.), p. 10–14. London and New York: Academic Press 1971
Page, S. E., Huxley, H. E.: Filament lengths in striated muscle. J. Cell Biol. 19, 369–390 (1963)
Sætersdal, T. S., Myklebust, R., Justesen, N. P. Berg, Olsen, W. Cato: Ultrastructural localization of calcium in the pigeon papillary muscle as demonstrated by cytochemical studies and X-ray microanalysis. Cell Tiss. Res. 155, 57–74 (1974)
Seveus, L., Kindel, L.: Dry cryo-sectioning of human and animal tissue at a very low temperature (-140° C). Eight Internat. Congress on electron microscopy, Canberra; Vol. II, 1974
Sjøstrøm, M., Thornell, L.-E.: Cryo-ultramicrotomy in the study of myofibrillar fine structure, II. The A-band. Science Tools 22 (1), 7–11 (1975)
Stockel, M. E., Hindelang-Gertner, C., Dellmann, H.-D., Porte, A., Stutinsky, F.: Subcellular localization of calcium in the mouse hypophysis. I. Calcium distribution in the adeno- and neurohypophysis under normal conditions. Cell Tiss. Res. 157, 307–322 (1975)
Tokuyasu, K. T.: A technique for ultracryotomy of cell suspensions and tissues. J. Cell Biol. 57, 551–565 (1973)
Worthington, C. R.: Large axial spacings in striated muscle. J. molec. Biol. 1, 398–401 (1959)
Yarom, R., Chandler, J. A.: Electron probe microanalysis of skeletal muscle. J. Histochem. Cytochem. 22(3), 147–154 (1974)
Yarom, R., Peters, P. D., Scripps, M., Rogel, S.: Effect of specimen preparation on intracellular myocardial calcium. Histochemistry 38, 143–153 (1974)
Yeh, B. K.: Localization of calcium antimonate in the atrial and ventrical muscle fibers of the cat heart. J. molec. Cell. Card. 5, 351–358 (1973)
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This work was supported by grants from The Norwegian Research Council for Science and the Humanities. We are also indebted to Mrs. Trine Jensen and Miss Sigrid Devik for skilful technical assistance.
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Myklebust, R., Berg Justesen, N.P. & Sætersdal, T.S. Troponin bound calcium in electron micrographs of myocardial cells as demonstrated by the potassium-antimonate technique. Cell Tissue Res. 162, 323–330 (1975). https://doi.org/10.1007/BF00220178
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DOI: https://doi.org/10.1007/BF00220178