Summary
A coating of electron dense material is present on the cytoplasmic surface of outer mitochondrial membranes in medium-sized hamster oocytes. The coating is not present at earlier or later stages of oocyte development. Its possible relationship to the synthesis and transport of mitochondrial protein is discussed. Associations between endoplasmic reticulum, ribosomes, glycogen and the outer mitochondrial membrane are also described and discussed.
Similar content being viewed by others
References
Alfert, M., Das, N.K., Eastwood, J.M.: Effects of ribonuclease on living cells and on cells fixed by different methods. J. Histochem. Cytochem. 10, 681–682 (1962)
Anderson, E.: Comparative aspects of the ultrastructure of the female gamete. Int. Rev. Cytol., Suppl. 4, 1–70 (1974)
Beams, H.W., Kessel, R.G.: Problem of germ cell determinants. Int. Rev. Cytol. 39, 413–479 (1974)
Brachet, J., Decroly, M., Ficq, A., Quertier, J.: Ribonucleic acid metabolism in unfertilized and fertilized sea urchin eggs. Biochim. biophys. Acta (Amst.) 72, 660–662 (1963)
Bracker, C.E., Grove, S.N.: Continuity between cytoplasmic endomembranes and outer mitochondrial membranes in fungi. Protoplasma 73, 15–34 (1971)
Butow, R.A., Bennett, W.F., Finkelstein, D.B., Kellems, R.E.: Nuclear cytoplasmic interactions in the biogenesis of mitochondria in yeast. In: Membrane biogenesis (A. Tzagoloff, ed.), pp. 155–199. New York: Plenum Press 1975
Clerot, J.-C.: Mise en évidence par cytochimie ultrastructurale de l'émission de protéines par le noyau d'auxocytes de Batraciens. J. Microscopie 7, 973–992 (1968)
Clerot, J.-C.: Étude autoradiographique de l'incorporation de la phenylalanine tritiée dans les groupements mitochondriaux du spermatocyte de Tanche. J. Microscopie 14, 32a (1972)
Cole, M.B.: Ultrastructural cytochemistry of granules associated with the nuclear pores of frog oocytes. J. Cell Biol. 43, 24a-25a (1969)
Conway, C.M.: Evidence for RNA in the heavy bodies of sea urchin eggs. J. Cell Biol. 51, 889–893 (1971)
Davidson, E.H.: Gene activity in early development. New York: Academic Press 1968
Davidson, E.H., Allfrey, V.G., Mirsky, A.E.: On the RNA synthesized during the lampbrush phase of amphibian oogenesis. Proc. nat. Acad. Sci. (Wash.) 52, 501–508 (1964)
Davidson, E.H., Crippa, M., Kramer, F.R., Mirsky, A.E.: Genomic function during the lampbrush chromosome stage of amphibian oogenesis. Proc. nat. Acad. Sci. (Wash.) 56, 856–863 (1966)
Davidson, E.H., Hough, B.R.: High sequence diversity in the RNA synthesized at the lampbrush stage of oogenesis. Proc. nat. Acad. Sci. (Wash.) 63, 342–349 (1969)
Davidson, E.H., Hough, B.R.: Genetic information in oocyte RNA. J. molec. Biol. 56, 491–506 (1971)
Denis, H., Mairy, M.: Recherches biochimiques sur l'oogenèse. I. Distribution intracellulaire du RNA dans les petits oocytes de Xenopus laevis. Europ. J. Biochem. 25, 524–534 (1972)
Dhainaut, A.: Étude en microscopie électronique et par autoradiographie a haute résolution des extrusions nucléaires au cours de l'ovogénèse de Nereis pelagica (Annelide Polychète). J. Microscopie 9, 99–118 (1970)
Eddy, E.M.: Fine structural observations on the form and distribution of nuage in germ cells of the rat. Anat. Rec. 178, 731–758 (1974)
Eddy, E.M.: Germ plasm and the differentiation of the germ cell line. Int. Rev. Cytol. 43, 229–280 (1975)
Eddy, E.M., Ito, S.: Fine structural and radioautographic observations on dense perinuclear cytoplasmic material in tadpole oocytes. J. Cell Biol. 49, 90–108 (1971)
Ernster, L., Kuylenstierna, B.: Structure, composition and function of mitochondrial membranes. In: Mitochondria: Structure and function (L. Ernster and Z. Drahota, eds.). London-New York: Academic Press 1969
Ficq, A.: RNA synthesis in early oogenesis of Xenopus laevis. Exp. Cell Res. 63, 453–457 (1970)
Ford, P.J.: Non-coordinated accumulation and synthesis of 5S ribonucleic acid by ovaries of Xenopus laevis. Nature (Lond.) 233, (1971)
Franke, W.W., Kartenbeck, J.: Outer mitochondrial membrane continuous with endoplasmic reticulum. Protoplasma 73, 35–41 (1971)
Gall, J.G., Pardue, M.L.: Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc. nat. Acad. Sci. (Wash.) 63, 378–383 (1969)
Georgiev, G.P., Samarina, O.P.: D-RNA containing ribonucleoprotein particles. Adv. Cell Biol. 2, 47–110 (1971)
Gerin, Y.: Étude par cytochimie ultrastructurale des corpuscules périnucléaires présents dans les jeunes oocytes de Ilyanassa obsoleta Say (Mollusca gastéropode). J. Embryol. exp. Morph. 25, 423–438 (1971)
Guelin, M.: Présence d'ARN dans le material dense périnuclèaire des trophocytes d'Ephestia kéhniella (Insecte, Lepidoptere). J. Microscopie 14, 52a (1972)
Halkka, L., Halkka, O.: Accumulation of gene products in the oocytes of the dragonfly Cordulia aenea L. I. The nematosomes. J. Cell Sci. 19, 103–115 (1975)
Hay, E.D.: Polygranular arrays of glycogen that mimic polyribosomes in Xenopus eggs and embryos. J. Cell Biol. 31, 45–46A (1966)
Hereward, F.V.: Rough membranes in Schizosaccharomyces pombe protoplasts. Exp. Cell Res. 87, 213–218 (1974)
Iqbal, S.J., Weakley, B.S.: The effects of different preparative procedures on the ultrastructure of the hamster ovary. Histochemistry 38, 95–122 (1973)
Kalt, M.R.: Ultrastructural observations on the germ line of Xenopus laevis. Z. Zellforsch. 138, 41–62 (1973)
Kellems, R.E., Allison, V.F., Butow, R.A.: Cytoplasmic type 80S ribosomes associated with yeast mitochondria. II. Evidence for the association of cytoplasmic ribosomes with the outer mitochondrial membrane in situ. J. biol. Chem. 249, 3297–3303 (1974)
Kellems, R.E., Allison, V.F., Butow, R.A.: Cytoplasmic type 80S ribosomes associated with yeast mitochondria. IV. Attachment of ribosomes to the outer membrane of isolated mitochondria. J. Cell Biol. 65, 1–14 (1975)
Kellems, R.E., Butow, R.A.: Cytoplasmic type 80S ribosomes associated with yeast mitochondria. I. Evidence for ribosome binding sites on yeast mitochondria. J. biol. Chem. 247, 8043–8050 (1972)
Kellems, R.E., Butow, R.A.: Cytoplasmic type 80S ribosomes associated with yeast mitochondria. III. Changes in the amount of bound ribosomes in response to changes in metabolic state. J. clin. Invest. 53, 3304–3310 (1974)
Keyhani, E.: Ribosomal granules associated with outer mitochondrial membrane in aerobic yeast cells. J. Cell Biol. 58, 480–484 (1973)
Leduc, E., Bernhard, W.: Recent modifications of the glycol methacrylate embedding procedure. J. Ultrastruct. Res. 19, 196–199 (1967)
Mahowald, A.P.: Polar granules of Drosophila. III. The continuity of polar granules during the life cycle of Drosophila. J. exp. Zool. 176, 329–344 (1971)
Mahowald, A.P., Hennen, S.: Ultrastructure of the “germ plasm” in eggs and embryos of Rana pipiens. Develop. Biol. 24, 37–53 (1971)
Mairy, M., Denis, H.: Recherches biochimiques sur l'oogenèse. I. Synthèse et accumulation du RNA pendant l'oogenèse du crapaud sud-Africain Xenopus laevis. Develop. Biol. 24, 143–165 (1971)
Mairy, M., Denis, H.: Recherches biochimiques fur l'oogenèse. II. Assemblage des ribosomes pendant le grand accroissement des oocytes de Xenopus laevis. Europ. J. Biochem. 25, 535–543 (1972)
Mazabraud, A., Wegnez, M., Denis, H.: Biochemical research on oogenesis. RNA accumulation in the oocytes of teleosts. Develop. Biol. 44, 326–332 (1975)
Morre, D.J., Merritt, W.D., Lembi, C.A.: Connections between mitochondria and endoplasmic reticulum in rat liver and onion stem. Protoplasma 73, 43–49 (1971)
Muramatsu, M., Utakoji, T., Sugano, H.: Rapidly labeled nuclear RNA in Chinese hamster testis. Exp. Cell Res. 53, 278–283 (1968)
Norrevang, A.: Electron microscopic morphology of oogenesis. Int. Rev. Cytol. 23, 113–186 (1968)
Petit, J.: Étude morphologique et cytochimique de deux types de groupements mitochondriaux dans les jeunes ovocytes. J. Microscopie 17, 41–54 (1973)
Rogers, A.W.: Autoradiography with the electron microscope. In: Techniques of autoradiography, Chap. 17. Amsterdam-London-New York: Elsevier Publishing Co. 1967
Ruby, J.R., Dyer, R.F., Skalko, R.G.: Continuities between mitochondria and endoplasmic reticulum in the mammalian ovary. Z. Zellforsch. 97, 30–37 (1969)
Schatz, G., Mason, T.L.: The biosynthesis of mitochondrial proteins. A. Rev. Biochem. 43, 51–87 (1974)
Smith, L.D., Williams, M.S.: Germinal plasm and determination of the primordial germ cells. In: The developmental biology of reproduction (C.L. Markert and J. Papaconstantinou, ed.), pp. 3–24. London-New York: Academic Press 1975
Sottocasa, G.L., Kuylenstierna, B., Ernster, L., Bergstrand, A.: An electron transport system associated with the outer membrane of liver mitochondria. J. Cell Biol. 32, 415–438 (1967a)
Sottocasa, G.L., Kuylenstierna, B., Ernster, L., Bergstrand, A.: Separation and some enzymatic properties of the inner and outer membranes of rat liver mitochondria. Meth. Enzym. 10, 448–463 (1967b)
Spirin, A.S.: On “masked” forms of messenger RNA in early embryogenesis and in other differentiating systems. In: Current topics in developmental biology, Vol. 1, pp. 1–38. London: Academic Press 1966
Spirin, A.S.: Informosomes. Europ. J. Biochem. 10, 20–35 (1969)
Thomas, C.: Évolution des structures ribosomiales au course de l'oogénèse chez Xenopus laevis. Arch. Biol. (Liège) 78, 347–369 (1967)
Thomas, C.: Étude cytochimique au microscope électronique des structures a ARN et du glycogène dans le cytoplasme des oocytes de Xenopus laevis. J. Embryol. exp. Morph. 21, 165–176 (1969)
Thomas, C.: Correlation between ultrastructural aspect of nucleoli and inhibition of ribosomal RNA synthesis in Xenopus laevis oocytes. Exp. Cell Res. 74, 547–551 (1972)
Thomas, C.: RNA metabolism in previtellogenic oocytes of Xenopus laevis. Develop. Biol. 39, 191–197 (1974)
Van Gansen, P., Schram, A.: Ultrastructure et cytochimie ultrastructurale de la vésicule germinative et du cytoplasme perinucléare de l'ovocyte mur de Xenopus laevis. J. Embryol. exp. Morph. 20, 375–389 (1968)
Van Gansen, P., Schram, A.: Incorporation of 3H-uridine and 3H-thymidine during the phase of nucleolar multiplication in Xenopus laevis oogenesis: a high resolution autoradiographic study. J. Cell Sci. 14, 85–103 (1974)
Weakley, B.S.: Initial stages in the formation of cytoplasmic lamellae in the hamster oocyte and the identification of electron dense particles. Z. Zellforsch. 97, 438–448 (1969)
Weakley, B.S.: Basic protein and ribonucleic acid in the cytoplasm of the ovarian oocyte in the golden hamster. Z. Zellforsch. 112, 69–84 (1971)
Weakley, B.S.: Variations in mitochondrial size and ultrastructure during germ cell development. Cell Tiss. Res. 169, (1976)
Webb, A.C.: An autoradiographic study of tritiated uridine incorporation into the larval ovary of Xenopus laevis. Anat. Rec. 184, (1976)
Wegnez, M., Denis, H.: Recherches biochimiques fur l'oogénèse. 4. Absence d'amplification des genes organisateurs du RNA 5S et du tRNA dans les petits oocytes de Xenopus laevis. Biochimie 54, 1069–1072 (1972)
Wegnez, M., Denis, H.: Recherches biochimiques fur l'oogénèse. 6. Propriétés du RNA 5S present dans les différents compartiments cellulaires des oocytes de Xenopus laevis. Biochimie 55, 1129–1135 (1973)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Weakley, B.S. Specializations of the outer mitochondrial membrane during oocyte development. Cell Tissue Res. 180, 515–528 (1977). https://doi.org/10.1007/BF00220172
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00220172