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Scanning electron microscopy of the glomerular filtration membrane in the rat kidney

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Summary

The rat kidney was perfused with saline and glutaraldehyde, treated with Murakami's tannin-osmium impregnation method, ethanol-freeze cracked and dried by the critical point method. Gold-palladium evaporated specimens were observed in a field-emission scanning electron microscope. The glomerular filtration membrane, fractured in different planes was observed with the following results:

  1. 1.

    Adjacent pedicles originate from different podocytes. No interpedicular bridges of apparent cytoplasmic nature could be found.

  2. 2.

    The basement membrane, in grazing fractures shows a horizontally layered architecture.

  3. 3.

    The attenuated endothelial sheet (lamina fenestrata) is divided into compartments, which we suggest should be called “areolae fenestratae”, by cytoplasmic crests radiating from the nucleated portion of the endothelial cell. A crest also occurs along the cell margin, which contacts a similar crest at the margin of the adjacent cell.

  4. 4.

    The pores in the areolae fenestratae are variable in size (30−150 nm diameter). A knob-like projection from the apparently naked basement membrane is found in a portion of the pores.

  5. 5.

    Numerous microvilli may occur on the endothelium. Some of them anastomose and fuse with one another to form a net whose meshes appear identical with the endothelial pores. Domes and shelves formed of a fenestrated cytoplasmic sheet also occur above the ordinary level of the endothelial lining. A hypothesis implicating microvilli in partial renewal of the endothelial sheet is proposed.

This study was assisted by Mr. K. Adachi of the SEM Laboratory at the Niigata University School of Medicine.

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References

  • Andrews, P.M., Porter, K.R.: A scanning electron microscopic study of the nephron. Amer. J. Anat. 140, 81–116 (1974)

    Google Scholar 

  • Arakawa, M.: A scanning electron microscopy of the glomerulus of normal and nephrotic rats. Lab. Invest. 23, 489–496 (1970)

    Google Scholar 

  • Arakawa, M., Tokunaga, J.: Further scanning electron microscope studies of the human glomerulus. Lab. Invest. 31, 436–440 (1974)

    Google Scholar 

  • Bloom, W., Fawcett, D.W.: A textbook of histology, 9th ed., p. 657. Philadelphia-London-Toronto: W.B. Saunders 1974

    Google Scholar 

  • Burkholder, P.M.: Atlas of human glomerular pathology. New York-Evanston-San Francisco-London: Harper & Row 1974

    Google Scholar 

  • Buss, H., Krönert, W.: Zur Struktur des Nierenglomerulum der Ratte. Rasterelektronenmikroskopische Untersuchungen. Virchows Arch. Abt. B 4, 79–92 (1969)

    Google Scholar 

  • Friederici, H.H.R.: The tridimensional ultrastructure of fenestrated capillaries. J. Ultrastruct. Res. 23, 444–456 (1968)

    Google Scholar 

  • Friederici, H.H.R.: On the diaphragm across fenestrae of capillary endothelium. J. Ultrastruct. Res. 27, 373–375 (1969)

    Google Scholar 

  • Fourman, J., Moffat, D.B.: The blood vessels of the kidney. Oxford and Edinburgh: Blackwell Sci. Publ. 1971

    Google Scholar 

  • Fujita, T., Tokunaga, J., Miyoshi, M.: Scanning electron microscopy of the podocytes of renal glomerulus. Arch. histol. jap. 32, 99–113 (1970)

    Google Scholar 

  • Groniowski, J., Biczyskowa, W., Walski, M.: Electron microscope studies on the surface coat of the nephron. J. Cell Biol. 40, 585–601 (1969)

    Google Scholar 

  • Hall, B.V.: Studies of normal glomerular structure by electron microscopy. In: Proc. 5th Ann. Conf. on the Nephrotic syndrome, p. 1–39. New York: National Nephrosis Foundation 1953

    Google Scholar 

  • Ham, A.W.: Histology, 7th ed., p. 760. Philadelphia and Toronto: J.B. Lippincott Co. 1974

    Google Scholar 

  • Hamano, M., Otaka, T., Nagatani, T., Tanaka, K.: A frozen liquid cracking method for high resolution scanning electron microscopy. (Abstract). J. Electron Micr. 22, 298 (1973)

    Google Scholar 

  • Humphreys, W.J., Spurlock, B.O., Johnson, J.S.: Critical point drying of ethanol-infiltrated, cryofractured biological specimens for scanning electron microscopy. In: Scanning electron microscopy 1974 (O. Johari, I. Corvin, eds.), p. 275–282. Chicago: IIT Res. Inst. 1974

    Google Scholar 

  • Kobayashi, S.: Occurrence of unique colloidal particles in snake blood and their transport across the capillary wall. A proposal of a new hypothesis on the permeability of the blood capillaries. Arch, histol. jap. 31, 511–528 (1970)

    Google Scholar 

  • Latta, H.: Ultrastructure of the glomerulus and juxtaglomerular apparatus. In: Handbook of physiology, Sect. 8: Renal physiology. (J. Orloff, R.W. Berliner, eds.), p. 1–29. Washington: American Physiological Society 1973

    Google Scholar 

  • Majno, G.: Ultrastructure of the vascular membrane. In: Handbook of physiology, Sect. 2: Circulation (W.F. Hamilton, ed.), vol. III, p. 2293–2375. Washington: American Physiological Society 1965

    Google Scholar 

  • Miyoshi, M., Fujita, T., Tokunaga, J.: The differentiation of renal podocytes. A combined scanning and transmission electron microscope study in rats. Arch. histol. jap. 33, 161–178 (1971)

    Google Scholar 

  • Murakami, T.: A revised tannin-osmium method for non-coated scanning electron microscope specimens. Arch. histol. jap. 36, 189–193 (1974)

    Google Scholar 

  • Pearse, D.C.: Fine structures of the kidney seen by electron microscopy. J. Histochem. Cytochem. 3, 295–308 (1955)

    Google Scholar 

  • Rhodin, J.A.G.: The diaphragm of capillary endothelial fenestration. J. Ultrastruct. Res. 6, 171–185 (1962)

    Google Scholar 

  • Simon, G.T., Chatelenat, F.: Ultrastructure of the normal and pathological glomerulus. In: The kidney (C. Rouiller, A.F. Muller, eds.), vol. 1, p. 261–349. New York-London: Academic Press 1969

    Google Scholar 

  • Suzuki, Y.: An electron microscopy of the renal differentiation. II. Glomerulus. Keio J. Med. 8, 129–144 (1959)

    Google Scholar 

  • Tokunaga, J., Edanaga, M., Fujita, T., Adachi, K.: Freeze cracking of scanning electron microscope specimens. A study of the kidney and spleen. Arch. histol. jap. 37, 165–182 (1974)

    Google Scholar 

  • Trump, B.F., Benditt, E.P.: Electron microscopic studies of human renal disease. Observations of normal visceral glomerular epithelium and its modification in disease. Lab. Invest. 11, 753–781 (1962)

    Google Scholar 

  • Wolff, J.: Elektronenmikroskopische Untersuchungen über die Vesikulation im Kapillarenendothel. Lokalisation, Variation und Fusion der Vesikel. Z. Zellforsch. 73, 143–164 (1966)

    Google Scholar 

  • Wolff, J., Merker, H.J.: Ultrastruktur und Bildung von Poren im Endothel von porösen und geschlossenen Kapillaren. Z. Zellforsch. 73, 174–191 (1966)

    Google Scholar 

  • Yamada, E.: The fine structure of the renal glomerulus of the mouse. J. biophys. biochem. Cytol. 1, 551–566 (1955)

    Google Scholar 

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Authors and Affiliations

  1. Department of Anatomy, Niigata University School of Medicine, Niigata

    T. Fujita, J. Tokunaga & M. Edanaga

  2. Department of Microbiology, Kyushu Dental College, Kitakyushu

    T. Fujita, J. Tokunaga & M. Edanaga

  3. Research Laboratories of Yoshitomi Pharmaceutical Industries, Ltd., Yoshitomi-cho, Fukuoka Prefecture, Japan

    T. Fujita, J. Tokunaga & M. Edanaga

Authors
  1. T. Fujita
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  2. J. Tokunaga
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  3. M. Edanaga
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Additional information

This paper is dedicated to Prof. Dr. med. W. Bargmann, my (T.F.) teacher in cell and tissue research and one of the pioneer microscopists in the study of renal glomerulus, on the occasion of his 70th birthday.

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Fujita, T., Tokunaga, J. & Edanaga, M. Scanning electron microscopy of the glomerular filtration membrane in the rat kidney. Cell Tissue Res. 166, 299–314 (1976). https://doi.org/10.1007/BF00220127

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  • DOI: https://doi.org/10.1007/BF00220127

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