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Receptor-mediated uptake of homologous low-density lipoproteins by isolated liver parenchymal cells of fetal rats

Cell and Tissue Research volume 254, pages 203–208 (1988)Cite this article

Summary

The binding and uptake of gold-labeled homologous, apolipoprotein E-free low-density lipoproteins (LDL) by isolated fetal rat liver parenchymal cells in suspension were studied ultrastructurally and morphometrically. Binding experiments using 125I-labeled LDL were also performed. After a 2-h preincubation in a lipoprotein-free medium and a subsequent 1-h postincubation in the presence of LDL-gold, fetal liver parenchymal cells exhibit a binding of 248±17 gold conjugates/100 μm plasma membrane and an uptake of 235±17 gold conjugates/100 μm2 cytoplasm. Compared with values obtained from freshly isolated nonpreincubated cells, these data correspond to a 15-fold and an 18-fold increase in total binding and uptake of LDL-gold, respectively. Competition experiments reveal that this increase is mainly a result of a 23-fold stimulation of specific binding and a 44-fold stimulation of receptor-mediated uptake of LDL-gold. The 125I-LDL binding experiments give a Kd value of 6.3×10-8 M and a maximum binding capacity of 17.3 fmol LDL/106 cells. Our data provide evidence, further to our in vivo studies, that fetal rat liver parenchymal cells possess high-affinity binding sites for native homologous apolipoprotein E-free LDL. These sites may correspond to B, E receptors of adult rat liver parenchymal cells.

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References

  • Berkel TJC van, Kruijt JK, Gent T van, Tol A van (1981) Saturable high affinity binding, uptake and degradation of rat plasma lipoproteins by isolated parenchymal and non-parenchymal cells from rat liver. Biochim Biophys Acta 665:22–33

    Google Scholar 

  • Brown MS, Goldstein JL (1984) How LDL receptors influence cholesterol and atherosclerosis. Sci Am 251:52–60

    Google Scholar 

  • Cooper AD (1985) Role of the liver in the degradation of lipoproteins. Gastroenterology 88:192–205

    Google Scholar 

  • Cooper AD, Nutik R, Chen J (1987) Characterization of estrogeninduced lipoprotein receptor of rat liver. J Lipid Res 28:59–68

    Google Scholar 

  • Devirgiliis LC, Dini L, Di Pierro A, Leoni S, Spagnuolo S, Stefanini S (1981) An improved non-perfusion method for the isolation and purification of rat foetal and neonatal hepatocytes. Cell Mol Biol 27:687–694

    Google Scholar 

  • Franke H, Dürer U, Schlag B, Dargel R (1987) In vivo binding and uptake of low-density lipoprotein-gold-and albumin-gold conjugates by parenchymal and sinusoidal cells of the fetal rat liver. Cell Tissue Res 249:221–226

    Google Scholar 

  • Griglio S (1986) Lipoprotein metabolism in isolated and cultured hepatocytes. In: Guillouzo A, Guguen-Guillouzo C (eds) Research in isolated and cultured hepatocytes. John Libbey Euro-text Ltd/INSERM, London Paris, pp 135–154

    Google Scholar 

  • Handley DA, Arbeeny CM, Eder H, Chien S (1981) Hepatic binding and internalization of low density lipoprotein-gold conjugates in rats treated with 17-α-ethinylestradiol. J Cell Biol 90:778–789

    Google Scholar 

  • Handley DA, Arbeeny CM, Chien S (1983) Sinusoidal endothelial endocytosis of low density lipoprotein-gold conjugates in perfused livers of ethinyl-estradiol treated rats. Eur J Cell Biol 30:266–271

    Google Scholar 

  • Harkes L, Berkel TJC van (1982) A saturable high-affinity binding site for human low-density lipoprotein on freshly isolated rat hepatocytes. Biochim Biophys Acta 712:677–683

    Google Scholar 

  • Harkes L, Berkel TJC van (1984) Quantitative role of parenchymal and non-parenchymal liver cells in the uptake of 14C-sucrose-labelled low-density lipoprotein in vivo. Biochem J 224:21–27

    Google Scholar 

  • Havel RJ (1985) Role of the liver in atherosclerosis. Arteriosclerosis 5:569–580

    Google Scholar 

  • Havel RJ (1986) Functional activities of hepatic lipoprotein receptors. Ann Rev Physiol 48:119–134

    Google Scholar 

  • Kovanen PT, Brown MS, Goldstein JL (1979) Increased binding of low density lipoprotein to liver membranes from rats treated with 17-α-ethinyl estradiol. J Biol Chem 254:11367–11373

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  • McFarlane AS (1963) In vivo behaviour of 131I-fibrinogen. J Clin Invest 42:346–356

    Google Scholar 

  • Mello RJ, Brown MS, Goldstein JL, Anderson RGW (1980) LDL receptors in coated vesicles isolated from bovine adrenal cortex: Binding sites unmasked by detergent treatment. Cell 20:829–837

    Google Scholar 

  • Mommaas-Kienhuis AM, Nagelkerke JF, Vermeer BJ, Daems WT, Berkel TJC van (1985) Visualization of the interaction of native and modified low density lipoproteins with isolated rat liver cells. Eur J Cell Biol 38:42–50

    Google Scholar 

  • Nilsson A (1977) Effects of anti-microtubular agents and cyclohexi-mide on the metabolism of chylomicron cholesteryl esters by hepatocyte suspensions. Biochem J 162:367–377

    Google Scholar 

  • Rudling MJ, Peterson CO (1985) LDL receptors in bovine tissues assayed as the heparin-sensitive binding of 125I-labeled LDL in homogenates: relation between liver LDL receptors and serum cholesterol in the fetus and post term. Biochim Biophys Acta 836:96–104

    Google Scholar 

  • Schlag B, Winkler L (1978) Concentration and composition of serum lipoproteins in the fetal rat. Acta Biol Med Germ 37:233–237

    Google Scholar 

  • Schlag B, Winkler L, Dürer U, Dargel R (1987) Preparation of apo E-free rat low density lipoprotein for catabolic studies. J Lipid Res 28:1521–1524

    Google Scholar 

  • Shepherd J, Bedford DK, Morgan HG (1976) Radioiodination of human low density lipoprotein: A comparison of four methods. Clin Chim Acta 66:97–109

    Google Scholar 

  • Stults NL, Lee YC (1986) Enhancement of galactose/N-acetyl-galactosamine receptor activity on the surface of freshly isolated rat hepatocytes. Evidence for masking of receptor sites by inhibitors derived from collagenase preparations. Proc Natl Acad Sci USA 83:7775–7779

    Google Scholar 

  • Weibel ER (1979) Stereological methods. Vol I, Practical methods for biological morphometry. Academic Press, London

    Google Scholar 

  • Zimmermann T, Franke H, Dargel R (1987) Isolation and characterization of parenchymal cells from normal and cirrhotic rat liver. Cell Biochem Funct 5:47–54

    CAS  PubMed  Google Scholar 

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Affiliations

  1. Institute of Pathological Biochemistry, Friedrich Schiller University, Jena, German Democratic Republic

    U. Dürer, M. Sommer, H. Franke, B. Schlag & R. Dargel

Authors
  1. U. Dürer
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  2. M. Sommer
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  3. H. Franke
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  4. B. Schlag
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  5. R. Dargel
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Dürer, U., Sommer, M., Franke, H. et al. Receptor-mediated uptake of homologous low-density lipoproteins by isolated liver parenchymal cells of fetal rats. Cell Tissue Res. 254, 203–208 (1988). https://doi.org/10.1007/BF00220035

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  • DOI: https://doi.org/10.1007/BF00220035

Key words

  • Fetal hepatocytes
  • Apo B, E receptors
  • Lipoproteins
  • Endocytosis
  • Rat (Wistar)