Summary
Electron-microscopic immunocytochemistry for the demonstration of CCK-like material in basal endocrine cells of the midgut of Aeschna cyanea, Locusta migratoria, Carausius morosus and Periplaneta americana was performed by use of the peroxidase-antiperoxidase procedure and the colloidal gold method. Immunoreactive cells appeared scattered among digestive and regenerative cells of the epithelium. Immunoreactivity was specifically detected over round to oval electron-dense granules whose size appeared rather different from species to species. Thus, the average size of 30% (d30) of the largest granules ranged from 312 nm in Periplaneta americana to 159 nm in Carausius morosus with intermediate values in Aeschna cyanea (d30=195 nm) and Locusta migratoria (d30=225 nm).
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Andriès, J.C., Beauvillain, J.C. Ultrastructural study of cholecystokinin-like immunoreactivity in endocrine cells of the insect midgut. Cell Tissue Res. 254, 75–81 (1988). https://doi.org/10.1007/BF00220019
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DOI: https://doi.org/10.1007/BF00220019