Summary
Previous studies have demonstrated an apparent differential response of the fiber types in mixed skeletal muscles of rats to streptozotocin diabetes. The purpose of the present study was to examine the ability of the different fiber types to hypertrophy in muscles from diabetic rats, which should further clarify the apparent differential trophic influence of insulin on the fibers. One group of rats was injected with streptozotocin to induce diabetes. The gastrocnemius muscle was then removed from one hindlimb of rats of both the diabetic and a second, normal group, resulting in compensatory growth of ipsilateral plantaris muscle. Rats were sacrificed 60 days following the surgery. Experimental muscles in normal and diabetic rats enlarged 79% and 61% over control muscles, respectively. In normal hypertrophied muscles there was an 8% increase in relative cross-sectional area composed of slow-twitch fibers, whereas in diabetic rats the slow-twitch component increased 17%. The results indicate that slow-twitch fibers in diabetic rats were capable of responding to the chronic power overloaded condition, but that the fast-twitch fibers had a reduced capacity to undergo compensatory growth. These findings support our previous observations suggesting that insulin may exert a differential trophic effect upon the muscle fiber types.
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Armstrong, R.B., Gollnick, P.D., Ianuzzo, C.D.: Histochemical properties of skeletal muscle fibers in streptozotocin-diabetic rats. Cell Tiss. Res. 162, 387–394 (1975)
Armstrong, R.B., Ianuzzo, C.D.: Decay of succinate dehydrogenase activity in rat skeletal muscle following streptozotocin injection. Horm. Met. Res. 8, 392–394 (1976)
Armstrong, R.B., Saubert IV, C.W., Sembrowich, W.L., Shepherd, R.E., Gollnick, P.D.: Glycogen depletion in rat skeletal muscle fibers at different intensities and durations of exercise. Pflügers Arch. 352, 243–256 (1974)
Arvill, A., Ahren, K.: Effects of insulin on the intact levator ani muscle of the rat. Biochem. biophys. Acta (Amst.) 135, 176–182 (1967)
Burke, R.E.: Group Ia synaptic input to fast and slow twitch motor units of cat triceps surae. J. Physiol. (Lond.) 196, 631–634 (1968)
Burke, R.E., Levine, D.N., Zajac III, F.E., Tsairis, R., Engel, W.K.: Mammalian motor units: physiological-histochemical correlation in three types in cat gastrocnemius. Science 174, 709–712 (1971)
Cooperstein, S.J., Lazarow, A., Kurfess, N.J.: A microspectrophotometric method for the determination of succinic dehydrogenase. J. biol. Chem. 186, 129–139 (1950)
Goldberg, A.: Role of insulin in work-induced growth of skeletal muscle. Endocrinology 83, 1071–1073 (1968)
Goldberg, A.L., Etlinger, J.D., Goldspink, D.F., Jablecki, C.: Mechanism of work-induced hypertrophy of skeletal muscle. Med. Sci. Sports. 7, 248–261 (1975)
Goldberg, A.L., Jablecki, C.M., Li, J.B.: Effects of use and disuse on animo acid transport and protein turnover in skeletal muscle. Ann. N.Y. Acad. Sci. 228, 190–201 (1974)
Goldspink, G., Larson, R.E., Davies, R.E.: The intermediate energy supply and the cost of maintenance of isometric tension for different muscles in the hamster. Z. vergl. Physiol. 66, 389–397 (1970)
Goldstein, M.S., Mullick, B., Huddlestun, B., Levine, R.: Action of muscular work on transfer of sugars across cell barriers: comparison with action of insulin. Amer. J. Physiol. 173, 212–215 (1953)
Guth, L., Yellin, H.: The dynamic nature of the so-called “fiber types” of mammalian skeletal muscle. Exp. Neurol. 31, 277–300 (1971)
Henneman, E., Olson, C.B.: Relations between structure and function in the design of skeletal muscles. J. Neurophysiol. 28, 581–598 (1965)
Holloszy, J.O., Oscai, L.B., Molé, P.A., Don, I.J.: Biochemical adaptations to endurance exercise in skeletal muscle. Muscle metabolism during exercise (B. Pernow and B. Saltin, eds.), pp. 51–61. New York: Plenum 1971
Hubbard, R.W., Ianuzzo, C.D., Matthew, W.T., Linduska, J.D.: Compensatory adaptations of skeletal muscle composition to a long-term functional overload. Growth 39, 85–93 (1975)
Ianuzzo, C.D., Armstrong, R.B.: DNA proliferation in normal and diabetic muscle during short term compensatory growth. Int. J. Biochem. 6, 889–892 (1975)
Ianuzzo, C.D., Armstrong, R.B.: Phosphofructokinase and succinate dehydrogenase activities of normal and diabetic rat skeletal muscle. Horm. Met. Res. 8, 244–245 (1976)
Ianuzzo, C.D., Gollnick, P.D., Armstrong, R.B.: Histochemical adaptations of rat skeletal muscle fibers to a long-term power overload condition. Life Sci. 19, 1517–1524 (1976)
Jablecki, C.K., Heuser, J.E., Kaufman, S.: Autoradiographic localization of new RNA synthesis in hypertrophying skeletal muscle. J. Cell Biol. 57, 743–759 (1973)
Manchester, K.L.: The control by insulin of amino acid accumulation in muscle. Biochem. J. 117, 457–465 (1970)
Manchester, K.L., Wool, I.G.: Insulin and incorporation of amino acids into protein of muscle. Biochem. J. 89, 202–213 (1963)
Novikoff, A.B., Shin, W., Drucker, J.: Mitochondrial localization of oxidative enzymes: staining results with two tetrazolium salts. J. biophys. biochem. Cytol. 9, 47–61 (1961)
Padykula, H.A., Herman, E.: The specificity of the histochemical method of adenosine triphosphatase. J. Histochem. Cytochem. 3, 170–195 (1955)
Peter, J.B., Barnard, R.J., Edgerton, V.R., Gillespie, C.A., Stempel, K.E.: Metabolic profiles of three fiber types of skeletal muscle in guinea pigs and rabbits. Biochem. 11, 2627–2634 (1972)
Saubert IV, C.W., Armstrong, R.B., Shepherd, R.E., Gollnick, P.D.: Anaerobic enzyme adaptations to sprint training in rats. Pflügers Arch. 341, 305–312 (1973)
Schiaffino, S., Bormioli, S.P.: Adaptive changes in developing rat skeletal muscle in response to functional overload. Exp. Neurol. 40, 126–137 (1973)
Shonk, C.E., Boxer, G.E.: Enzyme patterns in human tissue. I. Methods for the determination of glycolytic enzymes. Cancer Res. 24, 709–724 (1964)
Sola, O.M., Christensen, D.L., Martin, A.W.: Hypertrophy and hyperplasia of adult chicken anterior latissimus dorsi muscles following stretch with and without denervation. Exp. Neurol. 41, 76–100 (1973)
Thomas, P.K., Lascelles, R.G.: Schwann-cell abnormalities in diabetic neuropathy. Lancet I, 1355–1357 (1965)
Wattenberg, L.W., Leong, J.L.: Effects of coenzyme Q10 and menadione on succinate dehydrogenase activity as measured by tetrazolium salt reduction. J. Histochem. Cytochem. 8, 296–303 (1960)
Williams, P.E., Goldspink, G.: Longitudinal growth of striated muscle fibers. J. Cell Sci. 9, 751–767 (1971)
Wool, I.B., Stirewalt, W.S., Kurihara, K., Low, R.B., Bailey, P., Oyer, D.: Hormones and metabolic function-Mode of action of insulin in the regulation of protein biosynthesis in muscle. Recent Progr. Hormone Res. 24, 139–208 (1968)
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Streptozotocin was kindly donated by Dr. W.E. Dulin of the Upjohn Company. This investigation was supported by a Boston University Research Fund Grant
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Armstrong, R.B., Ianuzzo, C.D. Compensatory hypertrophy of skeletal muscle fibers in streptozotocin-diabetic rats. Cell Tissue Res. 181, 255–266 (1977). https://doi.org/10.1007/BF00219985
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DOI: https://doi.org/10.1007/BF00219985