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Nuclear characteristics of cardiac myocytes following the proliferative response to mincing of the myocardium in the adult newt, Notophthalmus viridescens

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Summary

Amphibian cardiac myocytes are predominantly mononucleated and have been demonstrated to respond to injury with DNA synthesis and mitosis. The nature of this response with regard to nuclear number and ploidy is unclear. In this study, the apex of the newt ventricle was minced and replaced, increasing the reactive area of the wound. At 45 days after mincing following multiple injections of tritiated thymidine (2.5-μCi/animal, 20 Ci/mM) 15 to 20 days after mincing, three ventricular zones were isolated and fixed: Zone 1, the minced area; Zone 2, extending approximately 500 μm proximally from the amputation plane; and Zone 3, the portion proximal to Zone 2. Myocytes separated in 50% KOH were examined for DNA synthesis by autoradiography and for nuclear number and DNA content using a scanning microdensitometer on Feulgen-Naphthol yellow S-stained cells. No labeled myocyte nuclei were found in control hearts and 98.3% of the myocytes were 2C. At 45 days, 46.78% of myocyte nuclei within Zone 1 were labeled, while 13% were non-diploid. In Zone 2, 9.25% were labeled with 4.8% non-diploid. In Zone 3, 1.1% were labeled, with 2.8% non-diploid. The newt ventricle's response to injury apparently may involve complete mitosis and cytokinesis, resulting in mononucleated diploid cells.

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References

  • Adler CP, Friedburg H (1986) Myocardial DNA content, ploidy level and cell number in geriatric hearts: Postmortem examinations of human myocardium in old age. J Mol Cell Cardiol 18:39–53

    Google Scholar 

  • Adler CP, Ringlage WP, Bohm N (1981) DNS-Gehalt und Zellzahl in Herz und Leber von Kindern. Pathol Res Pract 172:25–41

    Google Scholar 

  • Bader D, Oberpriller JO (1978) Repair and reorganization of minced cardiac muscle in the adult newt (Notophthalmus viridescens). J Morphol 155:349–358

    Google Scholar 

  • Bader D, Oberpriller JO (1979) Autoradiographic and electron microscopic studies of minced cardiac muscle regeneration in the adult newt Notophthalmus viridescens. J Exp Zool 208:177–194

    Google Scholar 

  • Bishop SP (1973) Effect of aortic stenosis on myocardial cell growth, hyperplasia and ultrastructure in neonatal dogs. Rec Adv Stud Card Struct Metab 3:637–656

    Google Scholar 

  • Brodsky Y, Uryvaeva IV (1977) Cell polyploidy: its relation to tissue growth and function. Int Rev Cytol 50:275–332

    CAS  PubMed  Google Scholar 

  • Brodsky Y, Uryvaeva IV (1985) Genome multiplication in growth and development. Biology of polyploid and polytene cells. Cambridge University Press, Cambridge

    Google Scholar 

  • Brodsky Y, Arefyeva AM, Uryvaeva IV (1980) Mitotic polyploidization of mouse heart myocytes during the first postnatal week. Cell Tissue Res 210:133–144

    Google Scholar 

  • Brodsky Y, Tsirekidze NN, Arefyeva AM (1985) Mitotic-cyclic and cycle-independent growth of cardiomyocytes. J Mol Cell Cardiol 17:445–455

    Google Scholar 

  • Bugaisky L, Zak R (1979) Cellular growth of cardiac muscle after birth. Tex Rep Biol Med 39:123–135

    Google Scholar 

  • Cantin M, Ballak M, Beuzeron-Mangina J, Anand-Srivastava MB, Tautu C (1981) DNA synthesis in cultured adult cardiocytes. Science 214:569–570

    Google Scholar 

  • Claycomb W, Bradshaw H (1983) Acquisition of multiple nuclei and the activity of DNA polymerase-α and reinitiation of DNA replication in terminally differentiated adult cardiac muscle cells in culture. Dev Biol 99:331–337

    Google Scholar 

  • Dow JW, Harding NGL, Powell T (1981) Isolated cardiac myocytes. I. Preparation of adult myocytes and their homology with the intact tissue. Cardiovasc Res 15:483–514

    Google Scholar 

  • Dowell RT, McManus RE (1978) Pressure induced cardiac enlargement in neonatal and adult rats: Left ventricular functional characteristics and evidence of cardiac cell proliferation in the neonate. Circ Res 42:303–310

    Google Scholar 

  • Kasten F, Kudriavtsev B, Rumyantsev P (1981) Fluorescent Feulgen-DNA content of isolated cardiac myocytes (Abstract). J Histochem Cytochem 29:886

    Google Scholar 

  • Katzberg AL, Farmer BA, Harris R (1977) The predominance of binucleation in isolated rat heart myocytes. Am J Anat 149:489–500

    Google Scholar 

  • Korecky B, Sweet S, Rakusan K (1979) Number of nuclei in mammalian cardiac myocytes. Can J Physiol Pharmacol 57:1122–1129

    Google Scholar 

  • Lash JW, Holtzer H, Swift H (1957) Regeneration of mature skeletal muscle. Anat Rec 128:679–698

    Google Scholar 

  • Mauro A (1979) Muscle regeneration. Raven Press, New York

    Google Scholar 

  • McGeachie JK (1971) Ultrastructural specificity in regenerating smooth muscle. Experientia 27:436

    Google Scholar 

  • Nag A, Cheng M, Fischman D, Zak R (1983) Long-term cell culture of adult mammalian cardiac myocytes. Electron microscopic and immunofluorescent analysis of myofibrillar structure. J Mol Cell Cardiol 15:301–317

    Google Scholar 

  • Neffgen JF, Korecky B (1972) Cellular hyperplasia and hypertrophy in cardiomegalies induced by anemia in young and adult rats. Circ Res 30:104–113

    Google Scholar 

  • Oberpriller JO, Oberpriller JC (1974) Response of the adult newt ventricle to injury. J Exp Zool 187:249–259

    Google Scholar 

  • Oberpriller JO, Oberpriller JC (1985) Cell division in cardiac myocytes. In: Ferrans VJ, Rosenquist GC, Weinstein C (eds) Cardiac morphogenesis. Elsevier, New York, pp 12–22

    Google Scholar 

  • Oberpriller JO, Oberpriller JC, Bader DM, McDonnell TJ (1981) Cardiac muscle and its potential for regeneration in the adult newt heart. In: Becker RO (ed) Mechanisms of growth control. Charles C. Thomas, Springfield IL, pp 343–372

    Google Scholar 

  • Oberpriller JO, Ferrans VJ, Carroll RJ (1983) Changes in DNA content, number of nuclei and cellular dimensions of young rat atrial myocytes in response to left coronary artery ligation. J Mol Cell Cardiol 15:31–42

    Google Scholar 

  • Oberpriller JO, Ferrans VJ, Carroll RJ (1984) DNA synthesis in rat atrial myocytes as a response to left ventricular infarction. An autoradiographic study of enzymatically dissociated myocytes. J Mol Cell Cardiol 16:1119–1126

    Google Scholar 

  • Oberpriller JO, Ferrans VJ, McDonnell TJ, Oberpriller JC (1985) Activation of DNA synthesis and mitotic events in atrial myocytes following atrial and ventricular injury. In: Stone HL, Weglicki WB (eds) Pathobiology of cardiovascular injury. Martin Nijhoff Publishing, Boston, pp 410–421

    Google Scholar 

  • Oparil S (1985) Pathogenesis of ventricular hypertrophy. J Am Coll Cardiol 5:57B-65B

    Google Scholar 

  • Owens GK, Rabinovitch PS, Schwartz SM (1981) Smooth muscle cell hypertrophy versus hyperplasia in hypertension. Proc Natl Acad Sci USA 78:7759–7763

    Google Scholar 

  • Rumyantsev PP (1973) Post-injury DNA synthesis, mitosis, and ultrastructural reorganization of adult frog cardiac myocytes. An electron microscopic autoradiographic study. Z Zellforsch 139:431–450

    Google Scholar 

  • Rumyantsev PP (1977) Interrelations of the proliferation and differentiation processes during cardiac myogenesis and regeneration. Int Rev Cytol 51:187–273

    Google Scholar 

  • Rumyantsev PP (1981) New comparative aspects of myocardial regeneration with special reference to cardiomyocyte proliferative behavior. In: Becker RO (ed) Mechanisms of growth control. Charles C. Thomas, Springfield IL, pp 311–342

    Google Scholar 

  • Rumyantsev PP, Kassem AM (1976) Cumulative indices of DNA synthesizing myocytes in different compartments of the working myocardium and conductive system of the rats heart muscle following extensive left ventricular infarction. Virchows Arch (Cell Pathol) 20:329–342

    Google Scholar 

  • Rumyantsev PP, Mirakyan VO (1968) Increased activity of DNA synthesis and mitoses in rat atrial muscle cells under ventricular myocard infarction and local injuries of auricles. Tsitologiia 10:1276–1286

    Google Scholar 

  • Sandritter W, Scomazzoni G (1964) Deoxyribonucleic acid content (Feulgen photometry) and dry weight (interference microscopy) of normal and hypertrophic heart muscle fibres. Nature 202:100–101

    Google Scholar 

  • Tate JM, Oberpriller JO (1987) A light microscopic autoradiographic study of adult newt myocytes in cell culture. Anat Rec 218:135A-136A

    Google Scholar 

  • Tate JM, McDonnell TJ, Oberpriller JC, Oberpriller JO (1987) Isolation of cardiac myocytes from the adult newt, Notophthalmus viridescens. An electron microscopic and quantitative light microscopic analysis. Tissue Cell 19:577–585

    Google Scholar 

  • Zak R (1974) Development and proliferative capacity of cardiac muscle cells. Circ Res (Suppl II) 35:17–26

    Google Scholar 

Download references

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Oberpriller, J.O., Oberpriller, J.C., Arefyeva, A.M. et al. Nuclear characteristics of cardiac myocytes following the proliferative response to mincing of the myocardium in the adult newt, Notophthalmus viridescens . Cell Tissue Res. 253, 619–624 (1988). https://doi.org/10.1007/BF00219752

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